Down-Regulation of Key Virulence Factors Makes the Salmonella enterica Serovar Typhimurium rfaH Mutant a Promising Live-Attenuated Vaccine Candidate

• Published in: Infection and Immunity Vol. 74; no. 10; pp. 5914 - 5925
• Main Authors: Nagy, Gábor, Danino, Vittoria, Dobrindt, Ulrich, Pallen, Mark, Chaudhuri, Roy, Emödy, Levente, Hinton, Jay C, Hacker, Jörg
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.2006
• Subjects: Animals; Applied microbiology; Bacterial Proteins - genetics; Bacteriology; Biological and medical sciences; Cells, Cultured; Down-Regulation; Epithelial Cells - microbiology; Fundamental and applied biological sciences. Psychology; Humans; Lipopolysaccharides - biosynthesis; Macrophages - microbiology; Mice; Microbial Immunity and Vaccines; Microbiology; Miscellaneous; Mutation; Regulon - genetics; Salmonella enterica; Salmonella typhimurium - genetics; Salmonella typhimurium - growth & development; Salmonella typhimurium - metabolism; Salmonella Vaccines - genetics; Trans-Activators - genetics; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Attenuated - genetics; Virulence - genetics; Virulence Factors - biosynthesis; Virulence Factors - genetics; Microbiology; Virulence; Bacteria; Vaccine; Mutation; Attenuated strain; Immunity; Salmonella typhimurium; Enterobacteriaceae

Mutants of Salmonella enterica serovar Typhimurium that lack the transcriptional regulator RfaH are efficient as live oral vaccines against salmonellosis in mice. We show that the attenuation of the vaccine candidate strain is associated with reduced net growth in epithelial and macrophage cells. In...