Organoid culture to study epithelial cell differentiation and barrier formation in the colon: bridging the gap between monolayer cell culture and human subject research

• Published in: In vitro cellular & developmental biology. Animal Vol. 57; no. 2; pp. 174 - 190
• Main Authors: Varani, James, McClintock, Shannon D., Aslam, Muhammad Nadeem
• Format: Journal Article
• Language: English
• Published: New York Springer Science & Business Media LLC 01.02.2021; Springer US; Society for In Vitro Biology
• Subjects: Animal Genetics and Genomics; Animals; Biomedical and Life Sciences; Cell Biology; Cell Culture; Cell Culture Techniques; Cell Differentiation; Colon; Colon - cytology; Colon - ultrastructure; Consistency; Continuous culture; Developmental Biology; Differentiation (biology); Epithelial cells; Epithelial Cells - cytology; Human subjects; Humans; Immunology; INVITED REVIEW; Life Sciences; Monolayers; neoplasms; Organ Culture Techniques - methods; Organoids; Organoids - cytology; Organs; Research Subjects; Stem Cells; Tissue culture; Tissues; Tumors; Organoid; Adenoma; Colonic mucosa; Proteomics

Organoid culture provides a powerful technology that can bridge the gap between monolayer cell culture on the one hand and whole animal or human subject research on the other. Tissues from many different organs from multiple species, including human, have already been successfully adapted to organoi...