Increased Expression of Androgen Receptor Sensitizes Prostate Cancer Cells to Low Levels of Androgens

• Published in: Cancer research (Chicago, Ill.) Vol. 69; no. 20; pp. 8141 - 8149
• Main Authors: WALTERING, Kati K, HELENIUS, Merja A, SAHU, Biswajyoti, MANNI, Visa, LINJA, Marika J, JÄNNE, Olli A, VISAKORPI, Tapio
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.10.2009
• Subjects: Androgens - pharmacology; Antineoplastic agents; Antineoplastic Agents - pharmacology; Biological and medical sciences; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Blotting, Western; Cell Line, Tumor; Cell Proliferation - drug effects; Chromatin Immunoprecipitation; Dose-Response Relationship, Drug; Fluorescent Antibody Technique; Gene Expression Profiling; Gynecology. Andrology. Obstetrics; Humans; Male; Male genital diseases; Medical sciences; Neoplasms, Hormone-Dependent - drug therapy; Neoplasms, Hormone-Dependent - genetics; Neoplasms, Hormone-Dependent - metabolism; Nephrology. Urinary tract diseases; Oligonucleotide Array Sequence Analysis; Pharmacology. Drug treatments; Prostatic Neoplasms - drug therapy; Prostatic Neoplasms - genetics; Prostatic Neoplasms - metabolism; Purines - pharmacology; Receptors, Androgen - genetics; Receptors, Androgen - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Tumors; Tumors of the urinary system; Urinary tract. Prostate gland; Urinary system disease; Prostate disease; Androgen; Androgen receptor; Malignant tumor; Sex steroid hormone; Gene expression; Male genital diseases; Prostate cancer; Tumor cell; Cancer; Hormonal receptor

Androgen receptor (AR) is known to be overexpressed in castration-resistant prostate cancer. To interrogate the functional significance of the AR level, we established two LNCaP cell sublines expressing in a stable fashion two to four times (LNCaP-ARmo) and four to six times (LNCaP-ARhi) higher leve...