A Point Mutation in Nucleoside Diphosphate Kinase Results in a Deficient Light Response for Perithecial Polarity in Neurospora crassa

• Published in: The Journal of biological chemistry Vol. 276; no. 24; pp. 21228 - 21234
• Main Authors: Ogura, Yasunobu, Yoshida, Yusuke, Yabe, Naoto, Hasunuma, Kohji
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.06.2001; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Substitution; blue light; Cell Polarity; Cell Polarity - physiology; Cell Polarity - radiation effects; chemistry; cytology; Darkness; enzyme activity; enzymology; fungal anatomy; gene expression; genetics; Glutathione Transferase; Glutathione Transferase - metabolism; Histidine; irradiation; Light; metabolism; mutation; ndk-1 gene; Neurospora crassa; Neurospora crassa - cytology; Neurospora crassa - enzymology; Neurospora crassa - radiation effects; Nucleoside-Diphosphate Kinase; Nucleoside-Diphosphate Kinase - chemistry; Nucleoside-Diphosphate Kinase - genetics; Nucleoside-Diphosphate Kinase - metabolism; phenotype; Phosphorylation; phosphotransferases (kinases); physiology; Point Mutation; Proline; radiation effects; Recombinant Fusion Proteins; Recombinant Fusion Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; RNA, Messenger - genetics; Transcription, Genetic

In Neurospora crassa, the phosphorylation of nucleoside diphosphate kinase (NDK)-1 is rapidly enhanced after blue light irradiation. We have investigated the function of NDK-1 in the blue light signal transduction pathway. A mutant called psp (phosphorylation ofsmall protein) shows undetectable phos...