Discovery and Characterization of a New Family of Diterpene Cyclases in Bacteria and Fungi

• Published in: Angewandte Chemie International Edition Vol. 56; no. 17; pp. 4749 - 4752
• Main Authors: Yang, Yan‐long, Zhang, Shasha, Ma, Ke, Xu, Yuxing, Tao, Qiaoqiao, Chen, Yihua, Chen, Juan, Guo, Shunxing, Ren, Jinwei, Wang, Wenzhao, Tao, Yong, Yin, Wen‐Bing, Liu, Hongwei
• Format: Journal Article
• Language: English
• Published: WEINHEIM Wiley 18.04.2017; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: Agaricales - chemistry; Agaricales - enzymology; Agaricales - genetics; Agaricales - metabolism; Amino acid sequence; Bacteria; Bacteria - chemistry; Bacteria - enzymology; Bacteria - genetics; Bacteria - metabolism; Basidiocarps; Basidiomycetes; Basidiomycota - chemistry; Basidiomycota - enzymology; Basidiomycota - genetics; Basidiomycota - metabolism; biosynthesis; Chemistry; Chemistry, Multidisciplinary; cyclases; Cyclization; Dimethylallyltranstransferase - genetics; Dimethylallyltranstransferase - metabolism; diterpenes; Diterpenes - chemistry; Diterpenes - metabolism; E coli; Escherichia coli; Fungi; Genomes; Multigene Family; natural products; Nucleotide sequence; Phylogeny; Physical Sciences; Science & Technology; UbiA; natural products; APOPTOSIS; METABOLITES; HERICIUM-ERINACEUM; biosynthesis; SYNTHASES; cyclases; diterpenes; IDENTIFICATION; UbiA; CHLOROFLEXUS-AURANTIACUS

Diterpene cyclases from bacteria and basidiomycete fungi are seldom studied. Here, we presented the identification and verification of EriG, a member of the UbiA superfamily, as the enzyme responsible for the cyclization of the cyathane skeleton in the mushroom Hericium erinaceum. Genome mining usin...