Post-translation modification of proteins in tears

• Published in: Electrophoresis Vol. 31; no. 11; pp. 1853 - 1861
• Main Authors: You, Jingjing, Fitzgerald, Anna, Cozzi, Paul J, Zhao, Zhenjun, Graham, Peter, Russell, Pamela J, Walsh, Bradley J, Willcox, Mark, Zhong, Ling, Wasinger, Valerie, Li, Yong
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.06.2010; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: 2-DE; Aged; Aged, 80 and over; Dermcidin; Dyes; Electrophoresis, Gel, Two-Dimensional; Emerald; Eye Proteins - analysis; Eye Proteins - metabolism; Glycoprotein; Glycoproteins - analysis; Glycoproteins - metabolism; Glycosylation; Humans; Male; Mathematical models; Middle Aged; Peptides - analysis; Peptides - metabolism; Phosphorylation; Protein Processing, Post-Translational; Proteins; Reproducibility of Results; Ruby; Searching; Tandem Mass Spectrometry - methods; Tearing; Tears; Tears - chemistry; Tears - metabolism

This is the first 2-DE study using sequential dyes to analyse phospho-, glyco- and total tear protein profiles (Pro-Q Diamond for phosphoprotein, Pro-Q Emerald for glycoprotein and Sypro Ruby for total protein). This method minimised the gel-gel variations, allowing better comparisons among the thre...