Post-translation modification of proteins in tears

This is the first 2-DE study using sequential dyes to analyse phospho-, glyco- and total tear protein profiles (Pro-Q Diamond for phosphoprotein, Pro-Q Emerald for glycoprotein and Sypro Ruby for total protein). This method minimised the gel-gel variations, allowing better comparisons among the thre...

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Published in	Electrophoresis Vol. 31; no. 11; pp. 1853 - 1861
Main Authors	You, Jingjing, Fitzgerald, Anna, Cozzi, Paul J, Zhao, Zhenjun, Graham, Peter, Russell, Pamela J, Walsh, Bradley J, Willcox, Mark, Zhong, Ling, Wasinger, Valerie, Li, Yong
Format	Journal Article
Language	English
Published	Weinheim Wiley-VCH Verlag 01.06.2010 WILEY-VCH Verlag WILEY‐VCH Verlag
Subjects	2-DE Aged Aged, 80 and over Dermcidin Dyes Electrophoresis, Gel, Two-Dimensional Emerald Eye Proteins - analysis Eye Proteins - metabolism Glycoprotein Glycoproteins - analysis Glycoproteins - metabolism Glycosylation Humans Male Mathematical models Middle Aged Peptides - analysis Peptides - metabolism Phosphorylation Protein Processing, Post-Translational Proteins Reproducibility of Results Ruby Searching Tandem Mass Spectrometry - methods Tearing Tears Tears - chemistry Tears - metabolism
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Abstract	This is the first 2-DE study using sequential dyes to analyse phospho-, glyco- and total tear protein profiles (Pro-Q Diamond for phosphoprotein, Pro-Q Emerald for glycoprotein and Sypro Ruby for total protein). This method minimised the gel-gel variations, allowing better comparisons among the three profiles and generated a whole map of PTM profiles of tear protein. A novel tear protein, dermcidin, was identified for the first time in this study. The identification of this antimicrobial protein suggests a new model of defence in tears. In addition, we are able to present the first experimental evidence of the presence of glycosylated lipocalin 1 and cystatin S. Nucleobindin 2 was only detected using phospho staining, suggesting it is only phosphorylated in tears. This study provides the groundwork for understanding the PTM of tear proteins and consequently these methods could be useful in the search for biomarkers in tears.
AbstractList	This is the first 2-DE study using sequential dyes to analyse phospho-, glyco- and total tear protein profiles (Pro-Q Diamond for phosphoprotein, Pro-Q Emerald for glycoprotein and Sypro Ruby for total protein). This method minimised the gel-gel variations, allowing better comparisons among the three profiles and generated a whole map of PTM profiles of tear protein. A novel tear protein, dermcidin, was identified for the first time in this study. The identification of this antimicrobial protein suggests a new model of defence in tears. In addition, we are able to present the first experimental evidence of the presence of glycosylated lipocalin 1 and cystatin S. Nucleobindin 2 was only detected using phospho staining, suggesting it is only phosphorylated in tears. This study provides the groundwork for understanding the PTM of tear proteins and consequently these methods could be useful in the search for biomarkers in tears.
Author	Zhao, Zhenjun Walsh, Bradley J. Wasinger, Valerie Willcox, Mark Graham, Peter Li, Yong Zhong, Ling You, Jingjing Russell, Pamela J. Fitzgerald, Anna Cozzi, Paul J.
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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/20506419$$D View this record in MEDLINE/PubMed
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Snippet	This is the first 2-DE study using sequential dyes to analyse phospho-, glyco- and total tear protein profiles (Pro-Q Diamond for phosphoprotein, Pro-Q Emerald... This is the first 2‐DE study using sequential dyes to analyse phospho‐, glyco‐ and total tear protein profiles (Pro‐Q Diamond for phosphoprotein, Pro‐Q Emerald...
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SubjectTerms	2-DE Aged Aged, 80 and over Dermcidin Dyes Electrophoresis, Gel, Two-Dimensional Emerald Eye Proteins - analysis Eye Proteins - metabolism Glycoprotein Glycoproteins - analysis Glycoproteins - metabolism Glycosylation Humans Male Mathematical models Middle Aged Peptides - analysis Peptides - metabolism Phosphorylation Protein Processing, Post-Translational Proteins Reproducibility of Results Ruby Searching Tandem Mass Spectrometry - methods Tearing Tears Tears - chemistry Tears - metabolism
Title	Post-translation modification of proteins in tears
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