Effects of Enterococcus sp. isolated from deep seawater on inhibition of allergic responses in mice

• Published in: British journal of nutrition Vol. 102; no. 1; pp. 3 - 7
• Main Authors: Kondoh, Masatoshi, Hayashi, Atsushi, Okamori, Mariko, Motonaga, Chie, Enomoto, Tadao, Cheng, Lei, Shimada, Takashi
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 14.07.2009
• Subjects: Accumulation; Allergens; Allergens - administration & dosage; Allergies; Allergy; Anaphylaxis; Anaphylaxis - immunology; animal disease models; Animals; Anti-Allergic Agents - administration & dosage; Antigens, Bacterial - administration & dosage; Bacteria; Biological and medical sciences; Carbon dioxide; Cells; Cryptomeria; Cryptomeria japonica; Developing countries; Dose-Response Relationship, Drug; Dose–response relationships; Enterococcus; Enterococcus faecium - immunology; Enterococcus sp; eosinophilia; eosinophils; Eosinophils - immunology; Experiments; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; hypersensitive response; Hypersensitivity, Immediate - immunology; Hypersensitivity, Immediate - prevention & control; Immunoglobulin E - blood; inhibitors; Injections, Intraperitoneal; isolation; Laboratory animals; LDCs; Leukocyte Count; Marine; Mice; Mice, Inbred BALB C; Microbial Viability - immunology; Models, Animal; oral administration; Pollen; Probiotics; Seawater; Seawater - microbiology; Studies; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Viability; Water Microbiology; Enterococcus sp; Allergy; Dose–response relationships; Viability; Immunopathology; Vertebrata; Mammalia; Mouse; Animal; Rodentia; Inhibition; Dose; Dose―response relationships
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1017/S0007114508158998

The objective of the present study was to assess the effects of Enterococcus sp. strain TN-3 isolated from deep seawater on inhibition of eosinophil accumulation, IgE production and active cutaneous anaphylaxis (ACA). We investigated the effects of viable and non-viable TN-3 on allergen-induced peritoneal eosinophil accumulation in mice. Viable (5·4 × 1010 colony-forming units per 60 mg) or non-viable TN-3 (60 mg) was orally administered to BALB/c mice that had been sensitised with the cedar pollen (Cryptomeria japonica) allergen. Oral administration of non-viable TN-3 was effective in suppressing eosinophil accumulation while viable TN-3 was ineffective. We also examined the dose–response relationship for non-viable TN-3 in regard to eosinophil accumulation, IgE production and ACA in allergen-primed mice. Non-viable TN-3 was orally administered at doses of 15 mg (low dose), 30 mg (medium dose) and 60 mg (high dose) to BALB/c mice that had been sensitised with cedar pollen allergen. The anti-allergic effects expressed as inhibition of eosinophil accumulation, IgE production and ACA were found at the low and high doses, but not at the medium dose. These results suggest that non-viable TN-3 exhibited anti-allergic effects at doses of 15 and 60 mg.