NanoSIMS imaging reveals metabolic stratification within current-producing biofilms

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 116; no. 41; pp. 20716 - 20724
• Main Authors: Chadwick, Grayson L., Otero, Fernanda Jiménez, Gralnick, Jeffrey A., Bond, Daniel R., Orphan, Victoria J.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 08.10.2019; Proceedings of the National Academy of Sciences
• Subjects: Anaerobic respiration; Anodes; Biochemical Phenomena; Bioelectric Energy Sources; Biofilms; Biofilms - growth & development; Biological Sciences; Cell Respiration; Diffusion rate; Electric contacts; Electrical resistivity; Electricity; Electrochemistry; electrode reduction; Electrodes; extracellular electron transfer; Geobacter - growth & development; Geobacter - metabolism; Geobacter sulfurreducens; Image Processing, Computer-Assisted - methods; Mass spectrometry; Mass spectroscopy; Microorganisms; nanoSIMS; Nanotechnology; Nutrients; Oxidation-Reduction; Oxides; Potential gradient; Redox potential; Science & Technology - Other Topics; Secondary ion mass spectrometry; Spectrometry, Mass, Secondary Ion - methods; stable isotope tracers; Stable isotopes; Geobacter sulfurreducens; extracellular electron transfer; nanoSIMS; electrode reduction; stable isotope tracers

Metal-reducing bacteria direct electrons to their outer surfaces, where insoluble metal oxides or electrodes act as terminal electron acceptors, generating electrical current from anaerobic respiration. Geobacter sulfurreducens is a commonly enriched electricity-producing organism, forming thick con...