Peroxidase evolution in white-rot fungi follows wood lignin evolution in plants

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 116; no. 36; pp. 17900 - 17905
• Main Authors: Ayuso-Fernández, Iván, Rencoret, Jorge, Gutiérrez, Ana, Ruiz-Dueñas, Francisco Javier, Martínez, Angel T.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 03.09.2019
• Subjects: Agaricomycotina; Biodegradation; Biological Evolution; Biological Sciences; Catalysis; Degradation; Electron transfer; Enzymes; Evolution; Exposure; Fungi; Fungi - enzymology; Fungi - genetics; Genomes; Hydrolysis; Kinetics; Lignin; Lignin - analysis; Lignin - metabolism; Lignin peroxidase; Molecular structure; NMR; Nuclear magnetic resonance; Oxidation; Oxidation-Reduction; Peroxidase; Peroxidase - genetics; Peroxidase - metabolism; Plants - genetics; Plants - metabolism; Plants - microbiology; Polymers; Polyporales; Science & Technology - Other Topics; Softwoods; Solvents; Tryptophan; Two dimensional analysis; White rot; Wood; Wood - analysis; Wood - metabolism; Wood - microbiology; stopped-flow spectrophotometry; fungal evolution; ancestral peroxidases; NMR spectroscopy; lignin evolution

A comparison of sequenced Agaricomycotina genomes suggests that efficient degradation of wood lignin was associated with the appearance of secreted peroxidases with a solvent-exposed catalytic tryptophan. This hypothesis is experimentally demonstrated here by resurrecting ancestral fungal peroxidase...