Chemical synthesis rewriting of a bacterial genome to achieve design flexibility and biological functionality

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 116; no. 16; pp. 8070 - 8079
• Main Authors: Venetz, Jonathan E., Del Medico, Luca, Wölfle, Alexander, Schächle, Philipp, Bucher, Yves, Appert, Donat, Tschan, Flavia, Flores-Tinoco, Carlos E., van Kooten, Mariëlle, Guennoun, Rym, Deutsch, Samuel, Christen, Matthias, Christen, Beat
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 16.04.2019
• Series: PNAS Plus
• Subjects: Annotations; Bacteria; BASIC BIOLOGICAL SCIENCES; Biological Sciences; Caulobacter crescentus; Caulobacter crescentus - genetics; Caulobacter crescentus - physiology; chemical genome synthesis; Chemical synthesis; Codon - genetics; Codons; de novo DNA synthesis; Deoxyribonucleic acid; Design; DNA; DNA, Bacterial - chemical synthesis; DNA, Bacterial - genetics; Gene sequencing; Genes; Genes, Essential - genetics; Genetic Engineering - methods; genome rewriting; Genome, Bacterial - genetics; Genome, Bacterial - physiology; Genomes; Genomics; mRNA; Nucleotide sequence; Organic chemistry; Physical Sciences; PNAS Plus; synonymous recoding; Synthetic Biology - methods; Transposon mutagenesis; chemical genome synthesis; Caulobacter crescentus; de novo DNA synthesis; genome rewriting; synonymous recoding

Understanding how to program biological functions into artificial DNA sequences remains a key challenge in synthetic genomics. Here, we report the chemical synthesis and testing of Caulobacter ethensis-2.0 (C. eth-2.0), a rewritten bacterial genome composed of the most fundamental functions of a bac...