Genes for highly abundant proteins in Escherichia coli avoid 5’ codons that promote ribosomal initiation

• Published in: PLoS computational biology Vol. 19; no. 10; p. e1011581
• Main Authors: Lewin, Loveday E, Daniels, Kate G, Hurst, Laurence D
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 25.10.2023; Public Library of Science (PLoS)
• Subjects: Analysis; Bacteria; Biology and Life Sciences; Codon; Codons; E coli; Enrichment; Escherichia coli; Gene expression; Genes; Genetic aspects; Genetic engineering; Low noise; Medicine and Health Sciences; Mutation; Optimization; Proteins; Randomization; Research and Analysis Methods; Ribonucleic acid; RNA; Transfer RNA; Transgenes; United Kingdom

In many species highly expressed genes (HEGs) over-employ the synonymous codons that match the more abundant iso-acceptor tRNAs. Bacterial transgene codon randomization experiments report, however, that enrichment with such "translationally optimal" codons has little to no effect on the re...