Immobilization of N-carbamyl-D-amino acid amidohydrolase

• Published in: Bioscience, biotechnology, and biochemistry Vol. 62; no. 10; pp. 1839 - 1844
• Main Authors: Nanba, H. (Kaneko Seed Co. Ltd., Maebashi (Japan)), Ikenaka, Y, Yamada, Y, Yajima, K, Takano, M, Ohkubo, K, Hiraishi, Y, Yamada, K, Takahashi, S
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.10.1998; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ACIDE AMINE; AGROBACTERIUM; Amidohydrolases - biosynthesis; Amidohydrolases - chemistry; AMINO ACIDS; Amino Acids - chemical synthesis; AMINOACIDOS; Biological and medical sciences; Biotechnology; ENZIMAS INMOVILIZADAS; ENZYME IMMOBILISEE; Enzyme Stability; Enzymes, Immobilized - chemistry; ESCHERICHIA COLI; Escherichia coli - enzymology; Fundamental and applied biological sciences. Psychology; GENE; GENES; Hydrogen-Ion Concentration; IMMOBILISATION; IMMOBILIZATION; Immobilization of enzymes and other molecules; Immobilization techniques; IMMOBILIZED ENZYMES; INMOVILIZACION; LIASAS; LYASE; LYASES; Methods. Procedures. Technologies; N-carbamyl-D-amino acid amidohydrolase; Pseudomonas - enzymology; RESISTANCE A LA TEMPERATURE; RESISTENCIA A LA TEMPERATURA; Rhizobium - enzymology; TEMPERATURE RESISTANCE; thermotolerant; Stability; Support; Enzyme; Immobilization; Reducing agent; N-Carbamoyl-D-amino acid hydrolase; D-aminoacid; Agrobacterium; Enzymatic activity; Production; Bacteria; Hydrolases; Rhizobiaceae; Recombinant protein; Thermotolerance; Application; Immobilized enzyme; Comparative study

N-Carbamyl-D-amino acid amidohydrolase (DCase, produced with recombinant Escherichia colt cells using a cloned gene from Agrobacterium sp. Strain KNK712, has been immobilized for use in the production of D-amino acids. The porous polymers, Duolite A-568 and Chitopearl 3003, were much better than oth...