Conversion of hepatoma cells to hepatocyte-like cells by defined hepatocyte nuclear factors

• Published in: Cell research Vol. 29; no. 2; pp. 124 - 135
• Main Authors: Cheng, Zhuo, He, Zhiying, Cai, Yongchao, Zhang, Cheng, Fu, Gongbo, Li, Hengyu, Sun, Wen, Liu, Changcheng, Cui, Xiuliang, Ning, Beifang, Xiang, Daimin, Zhou, Tengfei, Li, Xiaofeng, Xie, Weifen, Wang, Hongyang, Ding, Jin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.02.2019; Nature Publishing Group
• Subjects: 13/1; 14/28; 14/63; 38/109; 38/44; 38/61; 38/77; 631/136/2435; 631/67/1059; 631/67/1504/1610; 64/60; Animals; Biomarkers; Biomedical and Life Sciences; Cancer; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Biology; Cell Line, Tumor; Cellular Reprogramming - genetics; Conversion; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450; Cytochromes P450; Drug metabolism; Forkhead protein; Fumarylacetoacetase; Gene expression; Genetic transformation; Genetic Vectors; Glycogen; HEK293 Cells; Hepatocellular carcinoma; Hepatocyte Nuclear Factor 1-alpha - genetics; Hepatocyte Nuclear Factor 1-alpha - metabolism; Hepatocyte Nuclear Factor 3-gamma - genetics; Hepatocyte Nuclear Factor 3-gamma - metabolism; Hepatocyte nuclear factor 4; Hepatocyte Nuclear Factor 4 - genetics; Hepatocyte Nuclear Factor 4 - metabolism; Hepatocytes; Hepatocytes - metabolism; Hepatoma; Heterografts; Humans; Life Sciences; Liver; Liver cancer; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Low density lipoprotein; Metabolism; Mice; Mice, Knockout; Mice, Nude; Morphology; Phenotype; Phenotypes; Physical characteristics; Proteins; Restoration; Secretion; Serum Albumin, Human - analysis; Transfection; Transplantation; Tumor Burden - genetics; Urea; Xenografts; Xenotransplantation

Normal cells become cancer cells after a malignant transformation, but whether cancer cells can be reversed to normal status remains elusive. Here, we report that the combination of hepatocyte nuclear factor 1A (HNF1A), HNF4A and forkhead box protein A3 (FOXA3) synergistically reprograms hepatocellu...