Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets

• Published in: Nature microbiology Vol. 5; no. 10; pp. 1299 - 1305
• Main Authors: Vogels, Chantal B. F., Brito, Anderson F., Wyllie, Anne L., Fauver, Joseph R., Ott, Isabel M., Kalinich, Chaney C., Petrone, Mary E., Casanovas-Massana, Arnau, Catherine Muenker, M., Moore, Adam J., Klein, Jonathan, Lu, Peiwen, Lu-Culligan, Alice, Jiang, Xiaodong, Kim, Daniel J., Kudo, Eriko, Mao, Tianyang, Moriyama, Miyu, Oh, Ji Eun, Park, Annsea, Silva, Julio, Song, Eric, Takahashi, Takehiro, Taura, Manabu, Tokuyama, Maria, Venkataraman, Arvind, Weizman, Orr-El, Wong, Patrick, Yang, Yexin, Cheemarla, Nagarjuna R., White, Elizabeth B., Lapidus, Sarah, Earnest, Rebecca, Geng, Bertie, Vijayakumar, Pavithra, Odio, Camila, Fournier, John, Bermejo, Santos, Farhadian, Shelli, Dela Cruz, Charles S., Iwasaki, Akiko, Ko, Albert I., Landry, Marie L., Foxman, Ellen F., Grubaugh, Nathan D.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2020; Nature Publishing Group
• Subjects: 45/77; 631/326/596/4130; 631/337; Analysis; Betacoronavirus - genetics; Betacoronavirus - isolation & purification; Biomedical and Life Sciences; Clinical Laboratory Techniques - methods; Clinical Laboratory Techniques - statistics & numerical data; Comparative analysis; Coronavirus Infections - diagnosis; Coronavirus Infections - epidemiology; Coronavirus Infections - virology; Coronaviruses; COVID-19; COVID-19 Testing; Genetic Variation; Genome, Viral; Humans; Infectious Diseases; Life Sciences; Medical Microbiology; Microbiology; Molecular Probe Techniques - statistics & numerical data; Pandemics; Parasitology; Pneumonia, Viral - diagnosis; Pneumonia, Viral - epidemiology; Pneumonia, Viral - virology; Public health; Reverse Transcriptase Polymerase Chain Reaction - methods; Reverse Transcriptase Polymerase Chain Reaction - statistics & numerical data; Reverse transcription; Ribonucleic acid; RNA; RNA - genetics; RNA Probes - genetics; RNA, Viral - analysis; RNA, Viral - genetics; SARS-CoV-2; Sensitivity and Specificity; Severe acute respiratory syndrome coronavirus 2; Virology

The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical and public health interventions. Currently, several quantitative reverse transcription–PCR (RT–qPCR) assays are being used by cl...