Coordinate regulation of cytochrome and alternative pathway respiration in tobacco

• Published in: Plant physiology (Bethesda) Vol. 100; no. 4; pp. 1846 - 1851
• Main Authors: Vanlerberghe, Greg C., McIntosh, Lee
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.12.1992
• Subjects: Actinomycin; Agronomy. Soil science and plant productions; antibioticos; antibiotics; antibiotique; Biological and medical sciences; breathing; cadena respiratoria; cell culture; Cell growth; chaine respiratoire; citocromos; Coordinate systems; cultivo de celulas; culture de cellule; cytochrome; Cytochromes; expresion genica; expression des genes; Fundamental and applied biological sciences. Psychology; gene expression; Gene expression regulation; Metabolism; Metabolism and Enzymology; Mitochondria; mitochondrie; mitocondria; Nicotiana tabacum; Oxidases; Oxidation; oxidoreductases; oxidoreductions; oxidorreductasas; oxirreducion; oxydoreductase; oxydoreduction; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; Plants; protein synthesis; respiracion; Respiration; respiratory chain; sintesis de proteinas; synthese proteique; Cell culture; Electron transfer; Stimulant plant; Metabolic inhibitor; Nicotiana tabacum; Immunological method; Proteins; Mitochondria; Regulation(control); Dicotyledones; Angiospermae; Isolation; Spermatophyta; Cyanide resistant respiration; Solanaceae
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.100.4.1846

In suspension cells of NT1 tobacco (Nicotiana tabacum L. cv bright yellow), inhibition of the cytochrome pathway of respiration with antimycin A induced a large increase in the capacity of the alternative pathway over a period of approximately 12 h, as confirmed in both whole cells and isolated mitochondria. The increase in alternative pathway capacity required de novo RNA and protein synthesis and correlated closely with the increase of a 35-kD alternative oxidase protein. When the cytochrome pathway of intact cells was inhibited by antimycin A, respiration proceeded exclusively through the alternative pathway, reached rates significantly higher than before antimycin A addition, and was not stimulated by p-trifluoromethoxycarbonylcyanide (FCCP). When inhibition of the cytochrome pathway was relieved, alternative pathway capacity and the level of the 35-kD alternative oxidase protein declined. Respiration rate also declined and could once again be stimulated by FCCP. These observations show that the capacities of the mitochondrial electron transport pathways can be regulated in a coordinate fashion.