Loss of SS18-SSX1 Inhibits Viability and Induces Apoptosis in Synovial Sarcoma

• Published in: Clinical orthopaedics and related research Vol. 472; no. 3; pp. 874 - 882
• Main Authors: Carmody Soni, Emily E., Schlottman, Silke, Erkizan, Hayriye V., Uren, Aykut, Toretsky, Jeffrey A.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.03.2014; Lippincott Williams & Wilkins Ovid Technologies
• Subjects: Adult; Apoptosis; Basic Research; Caspase 3 - metabolism; Cell Line, Tumor; Cell Survival; Child; Conservative Orthopedics; Down-Regulation; Female; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Humans; Male; Medicine; Medicine & Public Health; Middle Aged; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Oncogene Proteins, Fusion - genetics; Oncogene Proteins, Fusion - metabolism; Orthopedics; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Repressor Proteins - genetics; Repressor Proteins - metabolism; RNA Interference; Sarcoma, Synovial - genetics; Sarcoma, Synovial - metabolism; Sarcoma, Synovial - pathology; Signal Transduction; Sports Medicine; Surgery; Surgical Orthopedics; Symposium: Musculoskeletal Tumor Society 2012 Symposium; Translocation, Genetic; Synovial Sarcoma Cell; Protein Partner; Splice Variant; Synovial Sarcoma; shRNA Construct

Background Most synovial sarcomas contain a chromosomal translocation t(X;18), which results in the formation of an oncoprotein SS18-SSX critical to the viability of synovial sarcoma. Questions/purposes We (1) established and characterized three novel synovial sarcoma cell lines and asked (2) whethe...