Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

• Published in: AMB Express Vol. 10; no. 1; pp. 1 - 13
• Main Authors: Kanno, Alex Issamu, Leite, Luciana Cezar de Cerqueira, Pereira, Lennon Ramos, de Jesus, Mônica Josiane Rodrigues, Andreata-Santos, Robert, Alves, Rúbens Prince dos Santos, Durigon, Edison Luiz, Ferreira, Luís Carlos de Souza, Gonçalves, Viviane Maimoni
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 31.12.2019; Springer Nature B.V; SpringerOpen
• Subjects: Antibodies; Antigens; Biomass; Biomedical and Life Sciences; Bioreactors; Biotechnology; Cross-reactivity; Denaturation; Dengue fever; Diagnostic software; Diagnostic systems; E coli; Enzyme-linked immunosorbent assay; Epitopes; Heterologous protein production; Immunoglobulins; Infections; Life Sciences; Microbial Genetics and Genomics; Microbiology; Optimization; Original; Original Article; Response Surface Methodology; Serological diagnosis; Serological tests; Soluble expression; Viability; Viral diseases; Viral infections; Viruses; Zika NS1; Zika virus; Soluble expression; Response Surface Methodology; Zika NS1; Serological diagnosis; Heterologous protein production; E. coli

Diagnosing Zika virus (ZIKV) infections has been challenging due to the cross-reactivity of induced antibodies with other flavivirus. The concomitant occurrence of ZIKV and Dengue virus (DENV) in endemic regions requires diagnostic tools with the ability to distinguish these two viral infections. Re...