Structure of a paramyxovirus polymerase complex reveals a unique methyltransferase-CTD conformation

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 117; no. 9; pp. 4931 - 4941
• Main Authors: Abdella, Ryan, Aggarwal, Megha, Okura, Takashi, Lamb, Robert A., He, Yuan
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 03.03.2020
• Subjects: Animal diseases; Animal health; Antiviral agents; BASIC BIOLOGICAL SCIENCES; Biological Sciences; Catalytic Domain; Cryo-EM; Cryoelectron Microscopy; Crystallography; Crystallography, X-Ray; DNA-directed RNA polymerase; Domains; Enzymatic activity; Genome, Viral; Genomes; Methyltransferase; Methyltransferases - chemistry; Methyltransferases - genetics; Methyltransferases - metabolism; Models, Molecular; Nucleoproteins - chemistry; Nucleotides; Oligomerization; Parainfluenza; Parainfluenza Virus 5 - chemistry; Paramyxovirinae - enzymology; Paramyxovirinae - genetics; paramyxovirus; Phosphoproteins - chemistry; polymerase; Protein Binding; Protein Conformation; Protein Domains; Protein structure; replication; RNA polymerase; RNA viruses; RNA-directed RNA polymerase; Transcription; Viral Proteins - chemistry; Viral Proteins - metabolism; Viruses; X-ray crystallography; replication; transcription; Cryo-EM; polymerase; paramyxovirus

Paramyxoviruses are enveloped, nonsegmented, negative-strand RNA viruses that cause a wide spectrum of human and animal diseases. The viral genome, packaged by the nucleoprotein (N), serves as a template for the polymerase complex, composed of the large protein (L) and the homo-tetrameric phosphopro...