Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings

• Published in: 3 Biotech Vol. 6; no. 1; pp. 113 - 8
• Main Authors: Aanangi, Raveendra, Kotapati, Kasi Viswanath, Palaka, Bhagath Kumar, Kedam, Thyagaraju, Kanika, Nirmala Devi, Ampasala, Dinakara Rao
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2016; Springer Nature B.V
• Subjects: Agriculture; arachidonic acid; Bioinformatics; Biomaterials; Biotechnology; Cancer Research; Chemistry; Chemistry and Materials Science; circular dichroism spectroscopy; Enzymes; Fatty acids; Food; isoelectric point; Legumes; linoleate 13S-lipoxygenase; linoleic acid; linolenic acid; molecular weight; mung beans; Original; Original Article; Proteins; seed germination; seedlings; Seeds; Soybeans; Stem Cells; temperature; Vigna radiata; India; Plant lipoxygenases; Protein purification; Chromatofocusing; Circular dichroism; Mung bean

This study reports purification and characterization of lipoxygenase protein from mung bean germinating seedlings. Lipoxygenases (LOXs) are key enzymes in seed germination. The purified mung bean LOX has resolved into two peaks by chromatofocusing, one has highest LOX activity with an isoelectric po...