Quantitation of Aurora Kinase A Gene Copy Number in Urine Sediments and Bladder Cancer Detection

• Published in: JNCI : Journal of the National Cancer Institute Vol. 100; no. 19; pp. 1401 - 1411
• Main Authors: Park, Hong-Seok, Park, Weon Seo, Bondaruk, Jolanta, Tanaka, Noriyoshi, Katayama, Hiroshi, Lee, Sangkyou, Spiess, Philippe E., Steinberg, Jordan R., Wang, Zhi, Katz, Ruth L., Dinney, Colin, Elias, Keren J., Lotan, Yair, Naeem, Rizwan C., Baggerly, Keith, Sen, Subrata, Grossman, H. Barton, Czerniak, Bogdan
• Format: Journal Article
• Language: English
• Published: Cary, NC Oxford University Press 01.10.2008; Oxford Publishing Limited (England)
• Subjects: Aneuploidy; Aurora Kinase A; Aurora Kinases; Biological and medical sciences; Biomarkers, Tumor - genetics; Biomarkers, Tumor - urine; Bladder cancer; Cancer; Carcinoma in Situ - diagnosis; Carcinoma in Situ - genetics; Carcinoma, Transitional Cell - diagnosis; Carcinoma, Transitional Cell - enzymology; Carcinoma, Transitional Cell - genetics; Carcinoma, Transitional Cell - surgery; Carcinoma, Transitional Cell - urine; Cell Line, Tumor; Cystectomy; Diagnosis, Differential; DNA, Neoplasm - genetics; DNA, Neoplasm - urine; Gene Amplification; Gene expression; Gene Expression Regulation, Neoplastic; Green Fluorescent Proteins; Humans; Immunohistochemistry; In Situ Hybridization, Fluorescence; Kinases; Medical diagnosis; Medical sciences; Nephrology. Urinary tract diseases; Oncology; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - urine; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Transfection; Tumors; Tumors of the urinary system; Up-Regulation; Urinalysis; Urinary Bladder Neoplasms - diagnosis; Urinary Bladder Neoplasms - enzymology; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - surgery; Urinary Bladder Neoplasms - urine; Urinary tract. Prostate gland; Urogenital system; Human; Urine; Urinary system disease; Copy number; Aurora kinase; Urinary tract disease; Malignant tumor; Bladder cancer; Serine/threonine-protein kinase 6; Urology; Cancerology; Gene; Genetics; Bladder disease; Diagnosis; Cancer

Background Chromosome missegregation and the resulting aneuploidy is a common change in neoplasia. The Aurora kinase A (AURKA) gene, which encodes a key regulator of mitosis, is frequently amplified and/or overexpressed in cancer cells, and the level of AURKA amplification is associated with the lev...