OCT4/SOX2-independent Nanog autorepression modulates heterogeneous Nanog gene expression in mouse ES cells

• Published in: The EMBO journal Vol. 31; no. 24; pp. 4547 - 4562
• Main Authors: Navarro, Pablo, Festuccia, Nicola, Colby, Douglas, Gagliardi, Alessia, Mullin, Nicholas P, Zhang, Wensheng, Karwacki-Neisius, Violetta, Osorno, Rodrigo, Kelly, David, Robertson, Morag, Chambers, Ian
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 12.12.2012; Nature Publishing Group UK; Blackwell Publishing Ltd; EMBO Press; Nature Publishing Group
• Subjects: Animals; Biochemistry, Molecular Biology; Cell Differentiation - genetics; Cell Differentiation - physiology; Cellular Biology; Chromatin Immunoprecipitation; EMBO09; EMBO11; Embryonic Stem Cells - metabolism; Feedback, Physiological; Flow Cytometry; Gene expression; Gene Expression Regulation - genetics; Gene Expression Regulation - physiology; Gene Regulatory Networks - genetics; Genomics; Green Fluorescent Proteins; Heterogeneity; Homeodomain Proteins - metabolism; In Situ Hybridization, Fluorescence; Life Sciences; Mice; Molecular biology; nanog; Nanog Homeobox Protein; network; pluripotency; Pluripotent Stem Cells - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Rodents; self-renewal; Signal transduction; Stem cells; Transcription Factors - metabolism; self‐renewal; nanog; pluripotency; heterogeneity; network

NANOG, OCT4 and SOX2 form the core network of transcription factors supporting embryonic stem (ES) cell self‐renewal. While OCT4 and SOX2 expression is relatively uniform, ES cells fluctuate between states of high NANOG expression possessing high self‐renewal efficiency, and low NANOG expression exh...