Proteolytic cleavage of glycoprotein B is dispensable for in vitro replication, but required for syncytium formation of pseudorabies virus

• Published in: Journal of General Virology Vol. 88; no. 7; pp. 1859 - 1865
• Main Author: Okazaki, K
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.07.2007; Microbiology Society; Society for General Microbiology
• Subjects: Animals; Biological and medical sciences; Cell Fusion; Cell Line; Cercopithecus aethiops; Chlorocebus aethiops; Dogs; Fundamental and applied biological sciences. Psychology; Furin; Furin - antagonists & inhibitors; Furin - metabolism; Giant Cells; Giant Cells - drug effects; Giant Cells - virology; glycoprotein B; glycoproteins; Herpesvirus 1, Suid; Herpesvirus 1, Suid - drug effects; Herpesvirus 1, Suid - pathogenicity; Herpesvirus 1, Suid - physiology; Humans; in vitro studies; microbial genetics; Microbiology; Miscellaneous; pathogenicity; protein subunits; proteinases; proteolysis; Pseudorabies virus; Rabbits; Serine Proteinase Inhibitors; Serine Proteinase Inhibitors - pharmacology; Suid alphaherpesvirus 1; Suid herpesvirus 1; Swine; Vero Cells; vertebrate viruses; Viral Envelope Proteins; Viral Envelope Proteins - metabolism; viral proteins; Virology; Virus Internalization; Virus Replication; Virus Replication - drug effects; Virus Replication - physiology; Virus; Aujeszky virus; Microbiology; Herpesviridae; In vitro replication; Alphaherpesvirinae; Glycoprotein; Virology

Glycoprotein B (gB) is the most conserved glycoprotein among herpesviruses and it plays important roles in virus infectivity. In most herpesviruses, including pseudorabies virus (PRV), gB is cleaved by a cellular protease into two disulfide-linked subunits. In the present study, I found that the PRV...