RNA-guided genome engineering: paradigm shift towards transposons

• Published in: Trends in biotechnology (Regular ed.) Vol. 42; no. 8; pp. 970 - 985
• Main Authors: Chang, Chin-Wei, Truong, Vy Anh, Pham, Nam Ngoc, Hu, Yu-Chen
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2024; Elsevier Limited
• Subjects: Animals; Bacteria; biotechnology; CASTs; CRISPR; CRISPR-Cas Systems; Design; DNA repair; DNA Transposable Elements - genetics; double-strand break; E coli; Editing; Efficiency; Engineering; family; Gene Editing - methods; Genetic engineering; Genetic Engineering - methods; genome; Genome editing; Genomes; Humans; Internal Medicine; OMEGA; Plasmids; Prokaryotes; Protein engineering; Proteins; Ribonucleic acid; RNA; RNA editing; RNA, Guide, CRISPR-Cas Systems - genetics; RNA-guided genome engineering; transposon; Transposons; transposon; CRISPR; double-strand break; CASTs; OMEGA; RNA-guided genome engineering

CRISPR-associated transposon (CAST) and obligate mobile element guided activity (OMEGA) systems derived from transposons are burgeoning RNA-guided genome engineering tools.CASTs are promising RNA-guided, double-strand break-free tools to engineer prokaryotes that are difficult to edit using conventi...