A Phytase Characterized by Relatively High pH Tolerance and Thermostability from the Shiitake Mushroom Lentinus edodes

• Published in: BioMed research international Vol. 2013; no. 2013; pp. 1 - 7
• Main Authors: Chen, Qing-Jun, Ng, Tzi Bun, Wu, Ying-Ying, Zhang, Guo-Qing, Wang, He-Xiang
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2013; John Wiley & Sons, Inc
• Subjects: 6-Phytase - chemistry; 6-Phytase - isolation & purification; Amino Acid Sequence; Chromatography; Enzyme Stability; Hydrogen-Ion Concentration; Ion exchange chromatography; Molecular Weight; Phosphatases; Physiological aspects; Properties; Shiitake; Shiitake Mushrooms - enzymology; Substrate Specificity; Temperature; China; Hong Kong, China

A monomeric phytase with a molecular mass of 14 kDa was acquired from fresh fruiting bodies of the shiitake mushroom Lentinus edodes. The isolation procedure involved chromatography on DEAE-cellulose, CM-cellulose, Q-Sepharose, Affi-gel blue gel, and a final fast protein liquid chromatography-gel fi...