Leucine supplementation accelerates connective tissue repair of injured tibialis anterior muscle
This study investigated the effect of leucine supplementation on the skeletal muscle regenerative process, focusing on the remodeling of connective tissue of the fast twitch muscle tibialis anterior (TA). Young male Wistar rats were supplemented with leucine (1.35 g/kg per day); then, TA muscles fro...
Saved in:
Published in | Nutrients Vol. 6; no. 10; pp. 3981 - 4001 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
29.09.2014
MDPI |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | This study investigated the effect of leucine supplementation on the skeletal muscle regenerative process, focusing on the remodeling of connective tissue of the fast twitch muscle tibialis anterior (TA). Young male Wistar rats were supplemented with leucine (1.35 g/kg per day); then, TA muscles from the left hind limb were cryolesioned and examined after 10 days. Although leucine supplementation induced increased protein synthesis, it was not sufficient to promote an increase in the cross-sectional area (CSA) of regenerating myofibers (p > 0.05) from TA muscles. However, leucine supplementation reduced the amount of collagen and the activation of phosphorylated transforming growth factor-β receptor type I (TβR-I) and Smad2/3 in regenerating muscles (p < 0.05). Leucine also reduced neonatal myosin heavy chain (MyHC-n) (p < 0.05), increased adult MyHC-II expression (p < 0.05) and prevented the decrease in maximum tetanic strength in regenerating TA muscles (p < 0.05). Our results suggest that leucine supplementation accelerates connective tissue repair and consequent function of regenerating TA through the attenuation of TβR-I and Smad2/3 activation. Therefore, future studies are warranted to investigate leucine supplementation as a nutritional strategy to prevent or attenuate muscle fibrosis in patients with several muscle diseases. |
---|---|
AbstractList | This study investigated the effect of leucine supplementation on the skeletal muscle regenerative process, focusing on the remodeling of connective tissue of the fast twitch muscle tibialis anterior (TA). Young male Wistar rats were supplemented with leucine (1.35 g/kg per day); then, TA muscles from the left hind limb were cryolesioned and examined after 10 days. Although leucine supplementation induced increased protein synthesis, it was not sufficient to promote an increase in the cross-sectional area (CSA) of regenerating myofibers (p > 0.05) from TA muscles. However, leucine supplementation reduced the amount of collagen and the activation of phosphorylated transforming growth factor-β receptor type I (TβR-I) and Smad2/3 in regenerating muscles (p < 0.05). Leucine also reduced neonatal myosin heavy chain (MyHC-n) (p < 0.05), increased adult MyHC-II expression (p < 0.05) and prevented the decrease in maximum tetanic strength in regenerating TA muscles (p < 0.05). Our results suggest that leucine supplementation accelerates connective tissue repair and consequent function of regenerating TA through the attenuation of TβR-I and Smad2/3 activation. Therefore, future studies are warranted to investigate leucine supplementation as a nutritional strategy to prevent or attenuate muscle fibrosis in patients with several muscle diseases. This study investigated the effect of leucine supplementation on the skeletal muscle regenerative process, focusing on the remodeling of connective tissue of the fast twitch muscle tibialis anterior (TA). Young male Wistar rats were supplemented with leucine (1.35 g/kg per day); then, TA muscles from the left hind limb were cryolesioned and examined after 10 days. Although leucine supplementation induced increased protein synthesis, it was not sufficient to promote an increase in the cross-sectional area (CSA) of regenerating myofibers ( p > 0.05) from TA muscles. However, leucine supplementation reduced the amount of collagen and the activation of phosphorylated transforming growth factor-β receptor type I (TβR-I) and Smad2/3 in regenerating muscles ( p < 0.05). Leucine also reduced neonatal myosin heavy chain (MyHC- n ) ( p < 0.05), increased adult MyHC-II expression ( p < 0.05) and prevented the decrease in maximum tetanic strength in regenerating TA muscles ( p < 0.05). Our results suggest that leucine supplementation accelerates connective tissue repair and consequent function of regenerating TA through the attenuation of TβR-I and Smad2/3 activation. Therefore, future studies are warranted to investigate leucine supplementation as a nutritional strategy to prevent or attenuate muscle fibrosis in patients with several muscle diseases. |
Author | Miyabara, Elen H Moriscot, Anselmo S Silva, Meiricris T Abrahamsohn, Paulo A Pereira, Marcelo G Carlassara, Eduardo O C Aoki, Marcelo S Gonçalves, Dawit A Kettelhut, Isis C |
AuthorAffiliation | 4 School of Arts, Sciences and Humanities, University of Sao Paulo, Arlindo Bettio Av. 1000, Sao Paulo, SP 03828-000, Brazil; E-Mail: aoki.ms@usp.br 3 Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 1524, Sao Paulo, SP 05508-000, Brazil; E-Mail: pauloabrahamsohn@gmail.com 1 Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 2415, Sao Paulo, SP 05508-000, Brazil; E-Mails: pereiramg@gmail.com (M.G.P.); me_tomaz@hotmail.com (M.T.S.); eduardo.carlassara@usp.br (E.O.C.C.); moriscot@usp.br (A.S.M.) 2 Department of Physiology and Biochemistry/Immunology, Ribeirao Preto Medical School, University of Sao Paulo, Bandeirantes Av. 3900, Ribeirao Preto, SP 14049-900, Brazil; E-Mails: dawitapg@yahoo.com.br (D.A.G.); idckette@fmrp.usp.br (I.C.K.) |
AuthorAffiliation_xml | – name: 2 Department of Physiology and Biochemistry/Immunology, Ribeirao Preto Medical School, University of Sao Paulo, Bandeirantes Av. 3900, Ribeirao Preto, SP 14049-900, Brazil; E-Mails: dawitapg@yahoo.com.br (D.A.G.); idckette@fmrp.usp.br (I.C.K.) – name: 3 Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 1524, Sao Paulo, SP 05508-000, Brazil; E-Mail: pauloabrahamsohn@gmail.com – name: 1 Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 2415, Sao Paulo, SP 05508-000, Brazil; E-Mails: pereiramg@gmail.com (M.G.P.); me_tomaz@hotmail.com (M.T.S.); eduardo.carlassara@usp.br (E.O.C.C.); moriscot@usp.br (A.S.M.) – name: 4 School of Arts, Sciences and Humanities, University of Sao Paulo, Arlindo Bettio Av. 1000, Sao Paulo, SP 03828-000, Brazil; E-Mail: aoki.ms@usp.br |
Author_xml | – sequence: 1 givenname: Marcelo G surname: Pereira fullname: Pereira, Marcelo G email: pereiramg@gmail.com organization: Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 2415, Sao Paulo, SP 05508-000, Brazil. pereiramg@gmail.com – sequence: 2 givenname: Meiricris T surname: Silva fullname: Silva, Meiricris T email: me_tomaz@hotmail.com organization: Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 2415, Sao Paulo, SP 05508-000, Brazil. me_tomaz@hotmail.com – sequence: 3 givenname: Eduardo O C surname: Carlassara fullname: Carlassara, Eduardo O C email: eduardo.carlassara@usp.br organization: Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 2415, Sao Paulo, SP 05508-000, Brazil. eduardo.carlassara@usp.br – sequence: 4 givenname: Dawit A surname: Gonçalves fullname: Gonçalves, Dawit A email: dawitapg@yahoo.com.br organization: Department of Physiology and Biochemistry/Immunology, School of Medicine, University of Sao Paulo, Bandeirantes Av. 3900, Ribeirao Preto, SP 14049-900, Brazil. dawitapg@yahoo.com.br – sequence: 5 givenname: Paulo A surname: Abrahamsohn fullname: Abrahamsohn, Paulo A email: pauloabrahamsohn@gmail.com organization: Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 1524, Sao Paulo, SP 05508-000, Brazil. pauloabrahamsohn@gmail.com – sequence: 6 givenname: Isis C surname: Kettelhut fullname: Kettelhut, Isis C email: idckette@fmrp.usp.br organization: Department of Physiology and Biochemistry/Immunology, School of Medicine, University of Sao Paulo, Bandeirantes Av. 3900, Ribeirao Preto, SP 14049-900, Brazil. idckette@fmrp.usp.br – sequence: 7 givenname: Anselmo S surname: Moriscot fullname: Moriscot, Anselmo S email: moriscot@usp.br organization: Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 2415, Sao Paulo, SP 05508-000, Brazil. moriscot@usp.br – sequence: 8 givenname: Marcelo S surname: Aoki fullname: Aoki, Marcelo S email: aoki.ms@usp.br organization: School of Arts, Sciences and Humanities, University of Sao Paulo, Arlindo Bettio Av. 1000, Sao Paulo, SP 03828-000, Brazil. aoki.ms@usp.br – sequence: 9 givenname: Elen H surname: Miyabara fullname: Miyabara, Elen H email: elenm@usp.br organization: Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Prof. Lineu Prestes Av. 2415, Sao Paulo, SP 05508-000, Brazil. elenm@usp.br |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25268835$$D View this record in MEDLINE/PubMed |
BookMark | eNpdkk1rFTEUhoNUbK1d-AdkwI0uruZjkkk2Qil-FC640XU8kzmpucwk12RS8N-beuulNZuEcx4eXk7Oc3ISU0RCXjL6TghD38eqGBVGsyfkjNOBb5TqxcmD9ym5KGVH785AByWekVMuudJayDPyY4vVhYhdqfv9jAvGFdaQYgfO4YwZViydSzGiW8MtdmsopWKXcQ8hd8l3Ie5qxqk1xgBzKB3EFXNIuVtqcTO-IE89zAUv7u9z8v3Tx29XXzbbr5-vry63GyeFXDeKgvd81MaMfhRcjd4LNeDQQmoKDHF0EukApu8np9iIVEvXS0bdYDx4Js7J9cE7JdjZfQ4L5N82QbB_CynfWMhraIksd4iTB1RU9z33BgYJbNR6GjTnHHxzfTi49nVccHJtKBnmR9LHnRh-2pt0a3vOqKGiCd7cC3L6VbGsdgmlzXOGiKkWy6QaqBSc0oa-_g_dpZpjG5VlivfaKKZMo94eKJdTKRn9MQyj9m4N7HENGvvqYfoj-e_TxR9JfLFn |
CitedBy_id | crossref_primary_10_1007_s00418_023_02194_5 crossref_primary_10_1089_dna_2020_5423 crossref_primary_10_3389_fmed_2021_755803 crossref_primary_10_1007_s00726_016_2240_1 crossref_primary_10_3390_cancers14010028 crossref_primary_10_3390_cells9122582 crossref_primary_10_3390_nu8070399 crossref_primary_10_1016_j_exger_2015_10_006 crossref_primary_10_1123_ijsnem_2017_0267 crossref_primary_10_1016_j_matpr_2020_11_466 crossref_primary_10_1038_srep18525 crossref_primary_10_1590_1806_9282_63_02_148 crossref_primary_10_1016_j_arr_2015_12_004 crossref_primary_10_1038_nature21034 crossref_primary_10_1038_s41536_021_00127_1 crossref_primary_10_1007_s10974_022_09615_3 crossref_primary_10_1292_jvms_18_0328 crossref_primary_10_1016_j_cmet_2024_02_014 crossref_primary_10_12965_jer_1632670_335 crossref_primary_10_1007_s10974_018_9495_y crossref_primary_10_1139_apnm_2016_0569 crossref_primary_10_1002_rco2_12 crossref_primary_10_3390_nu7053751 crossref_primary_10_1096_fj_202001798RR crossref_primary_10_1590_1414_431x2021e11597 crossref_primary_10_1113_EP085899 crossref_primary_10_3904_kjim_2020_197 crossref_primary_10_1177_0363546517702864 |
Cites_doi | 10.1016/S0021-9258(19)42013-9 10.1152/ajpcell.00399.2005 10.1371/journal.pone.0085283 10.3109/08977194.2011.595714 10.1152/ajpendo.00177.2003 10.1152/ajpcell.00248.2009 10.1016/j.clnu.2012.01.005 10.1055/s-0030-1270513 10.1111/j.1365-2818.1981.tb01200.x 10.1007/s00441-002-0519-x 10.1161/01.HYP.26.1.101 10.1186/1743-7075-9-67 10.1002/mus.21578 10.1093/jn/135.3.376 10.1679/aohc1950.41.267 10.1042/bj0990173 10.2174/138161210790883453 10.1093/jn/137.9.2074 10.1113/jphysiol.2006.110742 10.1055/s-2007-1019316 10.1002/mus.21754 10.1186/1471-2474-12-58 10.1074/jbc.M111.312488 10.1113/jphysiol.2003.056770 10.1152/ajpendo.00188.2011 10.1093/gerona/glr195 10.1007/BF00266843 10.1371/journal.pone.0012345 10.1079/PAR2004358 10.1016/0003-2697(76)90527-3 10.1016/S0934-8832(89)80021-6 10.1113/jphysiol.1995.sp020681 10.1152/physrev.00019.2003 10.1002/mus.22094 10.1038/hr.2013.96 10.1016/S0021-9258(19)68081-6 10.1152/ajpregu.00840.2010 10.1152/jappl.2001.91.2.534 10.1002/mnfr.201100695 10.1016/j.jbmt.2012.06.001 10.1152/ajpendo.00488.2005 10.3109/08977194.2011.595411 10.1242/dev.064162 |
ContentType | Journal Article |
Copyright | Copyright MDPI AG 2014 2014 by the authors; licensee MDPI, Basel, Switzerland. 2014 |
Copyright_xml | – notice: Copyright MDPI AG 2014 – notice: 2014 by the authors; licensee MDPI, Basel, Switzerland. 2014 |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 3V. 7TS 7X7 7XB 88E 8FI 8FJ 8FK ABUWG AFKRA AZQEC BENPR CCPQU DWQXO FYUFA GHDGH K9. M0S M1P PIMPY PQEST PQQKQ PQUKI PRINS 7X8 5PM DOA |
DOI | 10.3390/nu6103981 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef ProQuest Central (Corporate) Physical Education Index Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central ProQuest Central Essentials ProQuest Central ProQuest One Community College ProQuest Central Korea Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Health & Medical Complete (Alumni) Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Publicly Available Content Database ProQuest Central Essentials ProQuest One Academic Eastern Edition ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Central China ProQuest Hospital Collection (Alumni) Physical Education Index ProQuest Central ProQuest Health & Medical Complete Health Research Premium Collection ProQuest Medical Library ProQuest One Academic UKI Edition Health and Medicine Complete (Alumni Edition) ProQuest Central Korea ProQuest One Academic ProQuest Medical Library (Alumni) ProQuest Central (Alumni) MEDLINE - Academic |
DatabaseTitleList | MEDLINE Publicly Available Content Database |
Database_xml | – sequence: 1 dbid: DOA name: DOAJ Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: 7X7 name: Health & Medical Collection url: https://search.proquest.com/healthcomplete sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology |
EISSN | 2072-6643 |
EndPage | 4001 |
ExternalDocumentID | oai_doaj_org_article_2ceedfae608442f9a75a1b88d78222af 3494992921 10_3390_nu6103981 25268835 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GeographicLocations | Brazil |
GeographicLocations_xml | – name: Brazil |
GroupedDBID | --- 3V. 53G 5VS 7X7 88E 8FE 8FH 8FI 8FJ A8Z AADQD AAFWJ AAHBH AAWTL ABUWG ACIWK ACPRK ADRAZ AENEX AFKRA AFRAH AFZYC ALIPV ALMA_UNASSIGNED_HOLDINGS APEBS ATCPS BBNVY BENPR BHPHI BPHCQ BVXVI CCPQU CGR CUY CVF DIK E3Z EBD ECGQY ECM EIF EIHBH ESTFP EYRJQ F5P FYUFA GROUPED_DOAJ GX1 HCIFZ HMCUK HYE IPNFZ KQ8 LK8 M0K M1P M48 M7P MODMG M~E NPM OK1 P2P P6G PGMZT PIMPY PQQKQ PROAC PSQYO RIG RNS RPM TR2 UKHRP AAYXX CITATION 7TS 7XB 8FK AZQEC DWQXO K9. PQEST PQUKI PRINS 7X8 5PM AFPKN |
ID | FETCH-LOGICAL-c535t-60aff2b899bfb326bff367e783580a1eebc5e07a944dc61be085c4510c79faf13 |
IEDL.DBID | RPM |
ISSN | 2072-6643 |
IngestDate | Tue Dec 17 15:37:51 EST 2024 Tue Sep 17 21:24:32 EDT 2024 Fri Oct 25 07:08:18 EDT 2024 Thu Oct 10 16:27:42 EDT 2024 Fri Dec 06 02:44:50 EST 2024 Wed Oct 16 00:46:03 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 10 |
Language | English |
License | https://creativecommons.org/licenses/by/4.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c535t-60aff2b899bfb326bff367e783580a1eebc5e07a944dc61be085c4510c79faf13 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4210903/ |
PMID | 25268835 |
PQID | 1624896169 |
PQPubID | 2032353 |
PageCount | 21 |
ParticipantIDs | doaj_primary_oai_doaj_org_article_2ceedfae608442f9a75a1b88d78222af pubmedcentral_primary_oai_pubmedcentral_nih_gov_4210903 proquest_miscellaneous_1567053200 proquest_journals_1624896169 crossref_primary_10_3390_nu6103981 pubmed_primary_25268835 |
PublicationCentury | 2000 |
PublicationDate | 2014-09-29 |
PublicationDateYYYYMMDD | 2014-09-29 |
PublicationDate_xml | – month: 09 year: 2014 text: 2014-09-29 day: 29 |
PublicationDecade | 2010 |
PublicationPlace | Switzerland |
PublicationPlace_xml | – name: Switzerland – name: Basel |
PublicationTitle | Nutrients |
PublicationTitleAlternate | Nutrients |
PublicationYear | 2014 |
Publisher | MDPI AG MDPI |
Publisher_xml | – sequence: 0 name: MDPI AG – name: MDPI AG – name: MDPI |
References | Ciciliot (ref1) 2010; 16 Piérad (ref24) 1989; 9 Tischler (ref31) 1982; 257 ref34 Taillandier (ref7) 2004; 63 ref10 Cabello-Verrugio (ref18) 2012; 287 Mathieu (ref25) 1981; 121 Irintchev (ref36) 2002; 308 Junqueira (ref23) 1978; 41 Miyabara (ref5) 2010; 42 Katsanos (ref16) 2006; 291 Rogulska (ref19) 1975; 15 Nicoletti (ref47) 1995; 26 Baptista (ref14) 2010; 41 Rieu (ref17) 2006; 575 Vary (ref22) 2007; 137 Waalkes (ref29) 1957; 50 Bradford (ref32) 1976; 72 Hawinkels (ref48) 2011; 29 Turrina (ref44) 2013; 17 Lang (ref21) 2003; 285 ref46 ref26 Kaasik (ref45) 2011; 32 Wernig (ref35) 1995; 484 Miyabara (ref40) 2006; 290 ref42 Casperson (ref43) 2012; 31 Ten Broek (ref3) 2010; 224 Spector (ref15) 1977; 36 Gillies (ref12) 2011; 44 Haegens (ref41) 2012; 56 Buse (ref37) 1981; 13 Murphy (ref11) 2011; 138 Crozier (ref20) 2005; 135 ref8 Biernacka (ref13) 2011; 29 Fulks (ref30) 1975; 250 Scharff (ref28) 1966; 99 Hawke (ref39) 2001; 91 Conte (ref4) 2012; 67 Ge (ref6) 2009; 297 Charge (ref2) 2004; 84 Hao (ref38) 2011; 301 Gonçalves (ref27) 2012; 302 Ryall (ref33) 2004; 555 Duguez (ref9) 2003; 285 |
References_xml | – volume: 250 start-page: 290 year: 1975 ident: ref30 article-title: Effects of insulin, glucose, and amino acids on protein turnover in rat diaphragm publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)42013-9 contributor: fullname: Fulks – volume: 290 start-page: C1128 year: 2006 ident: ref40 article-title: Overexpression of inducible 70-kDa heat shock protein in mouse attenuates skeletal muscle damage induced by cryolesioning publication-title: Am. J. Physiol. Cell Physiol. doi: 10.1152/ajpcell.00399.2005 contributor: fullname: Miyabara – ident: ref10 doi: 10.1371/journal.pone.0085283 – volume: 29 start-page: 196 year: 2011 ident: ref13 article-title: TGF-beta signaling in fibrosis publication-title: Growth Factors doi: 10.3109/08977194.2011.595714 contributor: fullname: Biernacka – volume: 285 start-page: E1205 year: 2003 ident: ref21 article-title: Alcohol impairs leucine-mediated phosphorylation of 4E-BP1, S6K1, eIF4G, and mTOR in skeletal muscle publication-title: Am. J. Physiol. Endocrinol. Metab. doi: 10.1152/ajpendo.00177.2003 contributor: fullname: Lang – volume: 297 start-page: C1434 year: 2009 ident: ref6 article-title: mTOR regulates skeletal muscle regeneration in vivo through kinase-dependent and kinase-independent mechanisms publication-title: Am. J. Physiol. Cell Physiol. doi: 10.1152/ajpcell.00248.2009 contributor: fullname: Ge – volume: 31 start-page: 512 year: 2012 ident: ref43 article-title: Leucine supplementation chronically improves muscle protein synthesis in older adults consuming the RDA for protein publication-title: Clin. Nutr. doi: 10.1016/j.clnu.2012.01.005 contributor: fullname: Casperson – volume: 32 start-page: 247 year: 2011 ident: ref45 article-title: Extracellular matrix and myofibrils during unloading and reloading of skeletal muscle publication-title: Int. J. Sports Med. doi: 10.1055/s-0030-1270513 contributor: fullname: Kaasik – volume: 285 start-page: E206 year: 2003 ident: ref9 article-title: Myogenic and nonmyogenic cells differentially express proteinases, Hsc/Hsp70, and BAG-1 during skeletal muscle regeneration publication-title: Am. J. Physiol. contributor: fullname: Duguez – volume: 121 start-page: 75 year: 1981 ident: ref25 article-title: Measuring error and sampling variation in stereology: Comparison of the efficiency of various methods for planar image analysis publication-title: J. Microsc. doi: 10.1111/j.1365-2818.1981.tb01200.x contributor: fullname: Mathieu – volume: 308 start-page: 143 year: 2002 ident: ref36 article-title: Contractile properties, structure and fiber phenotype of intact and regenerating slow twitch muscles of mice treated with cyclosporin A publication-title: Cell Tissue Res. doi: 10.1007/s00441-002-0519-x contributor: fullname: Irintchev – volume: 26 start-page: 101 year: 1995 ident: ref47 article-title: Left ventricular fibrosis in renovascular hypertensive rats. Effect of losartan and spironolactone publication-title: Hypertension doi: 10.1161/01.HYP.26.1.101 contributor: fullname: Nicoletti – ident: ref42 doi: 10.1186/1743-7075-9-67 – volume: 41 start-page: 800 year: 2010 ident: ref14 article-title: Leucine attenuates skeletal muscle wasting via inhibition of ubiquitin ligases publication-title: Muscle Nerve doi: 10.1002/mus.21578 contributor: fullname: Baptista – volume: 135 start-page: 376 year: 2005 ident: ref20 article-title: Oral leucine administration stimulates protein synthesis in rat skeletal muscle publication-title: J. Nutr. doi: 10.1093/jn/135.3.376 contributor: fullname: Crozier – volume: 41 start-page: 267 year: 1978 ident: ref23 article-title: Differential staining of collagens type I, II and III by Sirius Red and polarization microscopy publication-title: Arch. Histol. Jpn. doi: 10.1679/aohc1950.41.267 contributor: fullname: Junqueira – volume: 99 start-page: 173 year: 1966 ident: ref28 article-title: Effect of diabetes on the concentration of amino acids in plasma and heart muscle of rats publication-title: Biochem. J. doi: 10.1042/bj0990173 contributor: fullname: Scharff – volume: 16 start-page: 906 year: 2010 ident: ref1 article-title: Regeneration of mammalian skeletal muscle. Basic mechanisms and clinical implications publication-title: Curr. Pharm. Des. doi: 10.2174/138161210790883453 contributor: fullname: Ciciliot – volume: 36 start-page: 1 year: 1977 ident: ref15 article-title: Leucine and alanine aminopeptidase activities in experimentally induced intradermal granulomas and late stages of wound healing in the rat publication-title: Lab. Investig. contributor: fullname: Spector – volume: 137 start-page: 2074 year: 2007 ident: ref22 article-title: Acute oral leucine administration stimulates protein synthesis during chronic sepsis through enhanced association of eukaryotic initiation factor 4G with eukaryotic initiation factor 4E in rats publication-title: J. Nutr. doi: 10.1093/jn/137.9.2074 contributor: fullname: Vary – volume: 575 start-page: 305 year: 2006 ident: ref17 article-title: Leucine supplementation improves muscle protein synthesis in elderly men independently of hyperaminoacidaemia publication-title: J. Physiol. doi: 10.1113/jphysiol.2006.110742 contributor: fullname: Rieu – volume: 13 start-page: 502 year: 1981 ident: ref37 article-title: In vivo effects of branched chain amino acids on muscle protein synthesis in fasted rats publication-title: Horm. Metab. Res. doi: 10.1055/s-2007-1019316 contributor: fullname: Buse – volume: 42 start-page: 778 year: 2010 ident: ref5 article-title: Mammalian target of rapamycin complex 1 is involved in differentiation of regenerating myofibers in vivo publication-title: Muscle Nerve doi: 10.1002/mus.21754 contributor: fullname: Miyabara – ident: ref8 doi: 10.1186/1471-2474-12-58 – volume: 287 start-page: 6773 year: 2012 ident: ref18 article-title: The internal region leucine-rich repeat 6 of decorin interacts with low density lipoprotein receptor-related protein-1, modulates transforming growth factor (TGF)-beta-dependent signaling, and inhibits TGF-beta-dependent fibrotic response in skeletal muscles publication-title: J. Biol. Chem. doi: 10.1074/jbc.M111.312488 contributor: fullname: Cabello-Verrugio – volume: 50 start-page: 733 year: 1957 ident: ref29 article-title: A fluorimetric method for estimation of tyrosine in plasma and tissue publication-title: J. Lab. Clin. Med. contributor: fullname: Waalkes – volume: 555 start-page: 175 year: 2004 ident: ref33 article-title: Beta 2 agonist administration reverses muscle wasting and improves muscle function in aged rats publication-title: J. Physiol. doi: 10.1113/jphysiol.2003.056770 contributor: fullname: Ryall – volume: 302 start-page: E123 year: 2012 ident: ref27 article-title: Clenbuterol suppresses proteasomal and lysosomal proteolysis and atrophy-related genes in denervated rat soleus muscles independently of Akt publication-title: Am. J. Physiol. Endocrinol. Metab. doi: 10.1152/ajpendo.00188.2011 contributor: fullname: Gonçalves – volume: 67 start-page: 443 year: 2012 ident: ref4 article-title: The beta2-adrenoceptor agonist formoterol improves structural and functional regenerative capacity of skeletal muscles from aged rat at the early stages of postinjury publication-title: J. Gerontol. A Biol. Sci. Med. Sci. doi: 10.1093/gerona/glr195 contributor: fullname: Conte – ident: ref46 doi: 10.1007/BF00266843 – ident: ref34 doi: 10.1371/journal.pone.0012345 – volume: 224 start-page: 7 year: 2010 ident: ref3 article-title: Regulatory factors and cell populations involved in skeletal muscle regeneration publication-title: J. Cell. Physiol. contributor: fullname: Ten Broek – volume: 15 start-page: 245 year: 1975 ident: ref19 article-title: Regeneration of crushed skeletal muscles in experimental animals and the effect of leucine on the course of this process in white rat publication-title: Pol. Med. Sci. Hist. Bull. contributor: fullname: Rogulska – volume: 63 start-page: 357 year: 2004 ident: ref7 article-title: The role of ubiquitin-proteasome-dependent proteolysis in the remodelling of skeletal muscle publication-title: Proc. Nutr. Soc. doi: 10.1079/PAR2004358 contributor: fullname: Taillandier – volume: 72 start-page: 248 year: 1976 ident: ref32 article-title: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding publication-title: Anal. Biochem. doi: 10.1016/0003-2697(76)90527-3 contributor: fullname: Bradford – volume: 9 start-page: 68 year: 1989 ident: ref24 article-title: Sirius red polarization method is used to visualize the organization of connective tissues but not the molecular composition of their fibrous polymers publication-title: Matrix doi: 10.1016/S0934-8832(89)80021-6 contributor: fullname: Piérad – volume: 484 start-page: 493 year: 1995 ident: ref35 article-title: Functional effects of myoblast implantation into histoincompatible mice with or without immunosuppression publication-title: J. Physiol. doi: 10.1113/jphysiol.1995.sp020681 contributor: fullname: Wernig – volume: 84 start-page: 209 year: 2004 ident: ref2 article-title: Cellular and molecular regulation of muscle regeneration publication-title: Physiol. Rev. doi: 10.1152/physrev.00019.2003 contributor: fullname: Charge – volume: 44 start-page: 318 year: 2011 ident: ref12 article-title: Structure and function of the skeletal muscle extracellular matrix publication-title: Muscle Nerve doi: 10.1002/mus.22094 contributor: fullname: Gillies – ident: ref26 doi: 10.1038/hr.2013.96 – volume: 257 start-page: 1613 year: 1982 ident: ref31 article-title: Does leucine, leucyltRNA or some metabolic of leucine regulate protein synthesis and degradation in skeletal and cardiac muscle? publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)68081-6 contributor: fullname: Tischler – volume: 301 start-page: R701 year: 2011 ident: ref38 article-title: β-Hydroxy-β-methylbutyrate reduces myonuclear apoptosis during recovery from hind limb suspension-induced muscle fiber atrophy in aged rats publication-title: Am. J. Physiol. Regul. Integr. Comp. Physiol. doi: 10.1152/ajpregu.00840.2010 contributor: fullname: Hao – volume: 91 start-page: 534 year: 2001 ident: ref39 article-title: Myogenic satellite cells: Physiology to molecular biology publication-title: J. Appl. Physiol. doi: 10.1152/jappl.2001.91.2.534 contributor: fullname: Hawke – volume: 56 start-page: 741 year: 2012 ident: ref41 article-title: Leucine induces myofibrillar protein accretion in cultured skeletal muscle through mTOR dependent and independent control of myosin heavy chain mRNA levels publication-title: Mol. Nutr. Food Res. doi: 10.1002/mnfr.201100695 contributor: fullname: Haegens – volume: 17 start-page: 95 year: 2013 ident: ref44 article-title: The muscular force transmission system: Role of the intramuscular connective tissue publication-title: J. Body. Mov. Ther. doi: 10.1016/j.jbmt.2012.06.001 contributor: fullname: Turrina – volume: 291 start-page: E381 year: 2006 ident: ref16 article-title: A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly publication-title: Am. J. Physiol. Endocrinol. Metab. doi: 10.1152/ajpendo.00488.2005 contributor: fullname: Katsanos – volume: 29 start-page: 140 year: 2011 ident: ref48 article-title: Exploring anti-TGF-beta therapies in cancer and fibrosis publication-title: Growth Factors doi: 10.3109/08977194.2011.595411 contributor: fullname: Hawinkels – volume: 138 start-page: 3625 year: 2011 ident: ref11 article-title: Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration publication-title: Development doi: 10.1242/dev.064162 contributor: fullname: Murphy |
SSID | ssj0000070763 |
Score | 2.240616 |
Snippet | This study investigated the effect of leucine supplementation on the skeletal muscle regenerative process, focusing on the remodeling of connective tissue of... |
SourceID | doaj pubmedcentral proquest crossref pubmed |
SourceType | Open Website Open Access Repository Aggregation Database Index Database |
StartPage | 3981 |
SubjectTerms | Angiogenesis Animals Collagen - drug effects Connective tissue Connective Tissue - drug effects Connective Tissue - metabolism Connective Tissue - pathology Dietary Supplements Electronic mail systems Growth factors Kinases Leucine - administration & dosage Leucine - pharmacology leucine supplementation Male Morphology Muscle, Skeletal - injuries Muscle, Skeletal - metabolism Musculoskeletal system Myofibrils - drug effects Myosin Heavy Chains - drug effects Protein synthesis Protein-Serine-Threonine Kinases - metabolism Proteins Rats Rats, Wistar Receptor, Transforming Growth Factor-beta Type I Receptors, Transforming Growth Factor beta - metabolism Regeneration - drug effects repair skeletal muscle injury Smad2 Protein - metabolism Smad2/3 Smad3 Protein - metabolism Spasm - diet therapy Tibia transforming growth factor-β receptor type I |
SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3PT9swFLamnrigDcbIKMhD025RY8fxj2NBVN0EO4HELbMdWysSKWqbA_897zmlahHSLrvGlvLyvdjve_bzZ0K-myhswxzPbSVkLhykOw7iaG60Yca6aLTDA843v-X0Tvy6r-63rvrCmrBeHrgHbsRxFo82yEILwaOxqrLMad2k0GZjmn0LvpVM9cRXQYJe9lJCJeT1o7aTuOup2U4ASjr975HLtzWSW0Fn8pHsr9kiHfdWfiIfQntADsctZMqPz_QHTfWbaWH8kPy5Dh3uktN0UWdfFI6o07H3EFtQEmJJU11LmuLobYKcAgO3swWdR_qzfegWoYEGPEUyW9Ixgj6bL-hNt4TXfyZ3k6vby2m-vj8h91VZrXJZ2Bi5g4zKRQc0zcVYShVwrUcXloXgfBUKZY0QjZfMBaBfXsAg9cpEG1l5RAbtvA3HhEpXGamUB36WBGec901pBPhBN6FwRUbOX0Gtn3qZjBrSC0S-3iCfkQuEe9MBla3TA_B3vfZ3_S9_Z2T46qx6PdyWNZNcaCOZNBn5tmmGgYK7H7YN8w76VFKlazDA2C-9bzeWcBS9AVgyona8vmPqbks7-5vEuAXH0tby6__4thOyB3xMYDkKN0MyWC26cAqcZ-XO0u_9AlT3A04 priority: 102 providerName: Directory of Open Access Journals – databaseName: Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Nb9QwELVKe-GCgBYILZVBqLeosePY8QktqFVBlFMr7S3Yjg1bqUmbbA7998w42cAixDWOZGvG9rz58BtC3usgTM0sT00hZCosuDsW7GiqS820sUGXFh84X36TF9fiy7JYTgG3fiqr3NyJ8aKuW4cx8lMmuSi1ZFJ_uLtPsWsUZlenFhqPyB7jYMphP6ulmmMskctG5iOhUA7e_WkzSMx9lmzLDEW2_n9BzL8rJf8wPedPyZMJM9LFqORnZMc3z8n-ogF_-faBntBYxRnD4_vk-1c_YK6cxnadY2k4yp4unAMLg8QQPY3VLfGio1dR8BRwuFl1tA30c3MzdL6GAXxLsurpAkW_ajt6OfQw_QG5Pj-7-nSRTl0UUlfkxTqVmQmBW_CrbLAA1mwIuVQeIz5lZpj31hU-U0YLUTvJrAcQ5gQcVad0MIHlL8hu0zb-FaHSFloq5QClRdoZ61yda2HBi659ZrOEvNsItbobyTIqcDJQ8tUs-YR8RHHPPyC_dfzQdj-q6bhUHG13MF5mpRA8aKMKw2CeOgIaExJytFFWNR26vvq9RRLydh6G44I5ENP4doB_CqliMwxY7MtRt_NKOFLfgFgSora0vrXU7ZFm9TNScguOBa756_8v65A8BrwlsNyE6yOyu-4G_wYwzdoex437C01c-fE priority: 102 providerName: ProQuest – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Nb9QwEB1V5cIFUcpHaEEGIW6BxHHs-IDQgqgKYjl1pd6C7diwCBLIbiT675lxsiuCeuQaO7Iz48m8Z49nAJ7pIEyTW56aUshUWKQ7Fv1oqiuda2ODrixdcF5-kucr8eGyvDyAXY3NSYCba6kd1ZNa9d9f_P519RoN_hUxTqTsL9tB0oEmXcC-wdEhUmTXckL5IwpWyNaLMa_Q_I2ZN4pJ-69Dmv8GTP7lgc5uw60JOrLFqOsjOPDtHThetEibf1yx5ywGc8Zd8mP4_NEPdGTOYtXOMUKcVMAWzqGjofwQGxaDXOL_jl1E-TOE42bdsy6w9-23ofcNNtCVkvWGLUgD665ny2GDw9-F1dm7i7fn6VRMIXVlUW5TmZkQuEV6ZYNFzGZDKKTytPFTZSb33rrSZ8poIRonc-sRizmBFuuUDibkxT04bLvWPwAmbamlUg7BWsw-Y51rCi0skunGZzZL4OlOqPXPMWdGjVyDJF_vJZ_AGxL3vgOluY4Puv5LPVlNzcmFB-NlVgnBgzaqNDmO00RcY0ICpztl1bulU-eSi0rLXOoEnuyb0WroKMS0vhuwTylVrImBk70_6nY_E04ZcFAsCaiZ1mdTnbe0668xM7fgFOdaPPwf33YCNxGcCYpN4foUDrf94B8hANrax3F5_wGQwwkR priority: 102 providerName: Scholars Portal |
Title | Leucine supplementation accelerates connective tissue repair of injured tibialis anterior muscle |
URI | https://www.ncbi.nlm.nih.gov/pubmed/25268835 https://www.proquest.com/docview/1624896169 https://search.proquest.com/docview/1567053200 https://pubmed.ncbi.nlm.nih.gov/PMC4210903 https://doaj.org/article/2ceedfae608442f9a75a1b88d78222af |
Volume | 6 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB615cIFUcoj0K4MQtzSzcOx4-O26gPEVhVqpb0F27FLEJtU2c2Bf8_YSZYu6olLDrEjWzPjzDf25xmAj8JSWcYqCWVGWUgVhjsK_WgochELqazIlbvgPL9il7f0yyJb7EA23oXxpH2tquP61_K4rn54buX9Uk9Hntj0en5KE0cnTKe7sIvu90GI3mNejrF52mcRSjGkn9YdcweeuasKk7j0Jrkv7vbXDfls_Y9BzH-Zkg9cz_lzeDZgRjLr57YPO6Z-AQezGuPl5W_yiXgWp98eP4DvX03nzsqJL9fZU8Od7MlMa_QwLjHEinh2i__RkRsveII4XFYtaSz5XP_sWlNig7tLUq3IzIm-aloy71Y4_Eu4PT-7Ob0MhyoKoc7SbB2ySFqbKIyrlFUI1pS1KePG7fjkkYyNUTozEZeC0lKzWBkEYZriUtVcWGnj9BXs1U1t3gBhKhOMc40ozaedUVqXqaAKo-jSRCoK4MMo1OK-T5ZRYJDhlFBslBDAiRP3poPLb-1fNO1dMWi5SJzvttKwKKc0sULyTMY4TukBjbQBHI7KKoZFtypiltBcsJiJAN5vmnG5uDMQWZumwz4Z474YBk72da_bzUxG2wiAb2l9a6rbLWihPiX3YJFv__vLd_AUoRh1TJREHMLeuu3MEcKdtZqgkS_4BJ6cnF1df5v4TQN8XixifM5pPvHm_wevnwgY |
link.rule.ids | 230,314,727,780,784,864,885,2102,2221,12056,21388,24318,27924,27925,31719,31720,33744,33745,43310,43805,53791,53793,73745,74302 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB7B9gCXCiiPQAGDELeoeThOfEJb1GoLuyuEtlJvwXZsWKQmJdkc-PfMONmFRYhrHMnWjO355uFvAN5Ix1UV6yRUGRch1-juaLSjoSxkLJV2stD0wHmxFLNL_uEquxoDbt1YVrm9E_1FXTWGYuQnsUh4IUUs5LubHyF1jaLs6thC4zYcEHN6NoGD07Plp8-7KItnsxHpQCmUon9_UveCsp9FvGeIPF__v0Dm37WSfxif83twOKJGNh3UfB9u2foBHE1r9Jivf7K3zNdx-gD5EXyZ256y5cw37ByKw0n6bGoM2hiihuiYr2_xVx1bedEzROJq3bLGsYv6e9_aCgfoNcm6Y1MS_rpp2aLvcPqHcHl-tno_C8c-CqHJ0mwTikg5l2j0rLTTCNe0c6nILcV8ikjF1mqT2ShXkvPKiFhbhGGG42E1uXTKxekjmNRNbZ8AEzqTIs8N4jRPPKONqVLJNfrRlY10FMDrrVDLm4Euo0Q3gyRf7iQfwCmJe_cDMVz7D037tRwPTJmQ9XbKiqjgPHFS5ZmKcZ7KQxrlAjjeKqscj11X_t4kAbzaDeOBoSyIqm3T4z-ZyH07DFzs40G3u5UkRH6DYgkg39P63lL3R-r1N0_KzRMqcU2f_n9ZL-HObLWYl_OL5cdncBfRF6fik0Qew2TT9vY5IpyNfjFu419uvf5H |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Nb9QwEB3BVkJcEFA-AgUMQtyizYfjxCe0ha5aaFcVaqXegu3YsEgkJdkc-PfMONnAIsQ1jmRrxva8sZ_fALyWjqsq1kmoMi5CrjHd0RhHQ1nIWCrtZKHpgfPZShxf8g9X2dXIf-pGWuV2T_QbddUYOiOfxyLhhRSxkHM30iLO3y_fXv8IqYIU3bSO5TRuwh5GxSiZwd7h0er803Ti4pVtRDrIC6WY68_rXtBNaBHvBCWv3f8vwPk3b_KPQLS8C3dGBMkWg8vvwQ1b34f9RY3Z8_ef7A3znE5_WL4Pn09tTzfnzBfvHIji5Am2MAbjDclEdMxzXfy2xy68GxiicrVuWePYSf2tb22FDfSyZN2xBTli3bTsrO-w-wdwuTy6eHccjjUVQpOl2SYUkXIu0ZhlaacRumnnUpFbOv8pIhVbq01mo1xJzisjYm0RkhmOC9fk0ikXpw9hVje1fQxM6EyKPDeI2bwIjTamSiXXmFNXNtJRAK-2Ri2vB-mMElMOsnw5WT6AQzL39AOpXfsPTfulHBdPmVAkd8qKqOA8cVLlmYqxn8rDG-UCONg6qxyXYFf-njABvJyacfHQjYiqbdPjP5nIfWkMHOyjwbfTSBISwkGzBJDveH1nqLst9fqrF-jmCdFd0yf_H9YLuIUzuDw9WX18CrcRiHHioSTyAGabtrfPEOxs9PNxFv8CCFsCgw |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Leucine+Supplementation+Accelerates+Connective+Tissue+Repair+of+Injured+Tibialis+Anterior+Muscle&rft.jtitle=Nutrients&rft.au=Marcelo+G.+Pereira&rft.au=Meiricris+T.+Silva&rft.au=Eduardo+O.+C.+Carlassara&rft.au=Dawit+A.+Gon%C3%A7alves&rft.date=2014-09-29&rft.pub=MDPI+AG&rft.eissn=2072-6643&rft.volume=6&rft.issue=10&rft.spage=3981&rft.epage=4001&rft_id=info:doi/10.3390%2Fnu6103981&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_2ceedfae608442f9a75a1b88d78222af |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2072-6643&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2072-6643&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2072-6643&client=summon |