Sucrose utilization during somatic embryo development in black spruce: involvement of apoplastic invertase in the tissue and of extracellular invertase in the medium

• Published in: Journal of plant physiology Vol. 162; no. 1; pp. 115 - 124
• Main Authors: Iraqi, Driss, Quy Le, Van, Lamhamedi, Mohammed S., Tremblay, Francine M.
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.01.2005; Elsevier; Elsevier Science Ltd
• Subjects: beta-fructofuranosidase; beta-Fructofuranosidase - metabolism; bioaccumulation; Biological and medical sciences; Black spruce; Carbohydrates; culture media; drug effects; Embryo development; embryology; Embryos; enzyme activity; enzymology; forest trees; Fructose; Fructose - metabolism; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - metabolism; Glucosyltransferases; Glucosyltransferases - metabolism; growth & development; hexoses; hydrolysis; Invertase; metabolism; pharmacology; Picea; Picea - drug effects; Picea - embryology; Picea - enzymology; Picea mariana; Plant physiology and development; Plant Proteins; plant tissues; Radioactivity; Seeds; Seeds - drug effects; Seeds - growth & development; Seeds - metabolism; somatic embryogenesis; somatic embryos; starch; Starch - metabolism; sucrose; Sucrose - metabolism; Sucrose - pharmacology; sucrose synthase; Sucrose utilization; Tissue cultures, protoplasts; Tissues; Sucrose utilization; Embryo development; Black spruce; Invertase; Enzyme; Starch; Transferases; β-Fructofuranosidase; Glycosyltransferases; Cell wall; Somatic embryo; Glycosidases; Gymnospermae; Development; Hydrolases; Coniferales; Hexosyltransferases; Spermatophyta; Carbohydrate; Sucrose synthase; O-Glycosidases; Picea mariana

The involvement of apoplastic invertase (Ap Inv) and sucrose synthase (SuSy) in the somatic embryo development of black spruce ( Picea mariana) was investigated under different maturation conditions. Replacing 6% sucrose with 3% or 1% sucrose in the maturation medium drastically decreased Ap Inv act...