Development and evaluation of a sandwich ELISA for quantification of the 20S proteasome in human plasma

• Published in: Journal of immunological methods Vol. 260; no. 1; pp. 183 - 193
• Main Authors: Dutaud, Dominique, Aubry, Laurent, Henry, Laurent, Levieux, Didier, Hendil, Klavs B., Kuehn, Lothar, Bureau, Jean Paul, Ouali, Ahmed
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.2002; Elsevier
• Subjects: Animals; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Biomarkers; Cancer; Cysteine Endopeptidases - blood; Cysteine Endopeptidases - immunology; ELISA (enzyme-linked immunosorbent assay); Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Hodgkin Disease - blood; Human; Human viral diseases; Humans; Infectious diseases; Leukemia, Lymphocytic, Chronic, B-Cell - blood; Medical sciences; Microbiology; Multienzyme Complexes - blood; Multienzyme Complexes - immunology; Pathological conditions; Plasma; Proteasome; proteasome 20S; Proteasome Endopeptidase Complex; Rabbits; Sensitivity and Specificity; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Human; Plasma; ELISA (enzyme-linked immunosorbent assay); Pathological conditions; Proteasome; Cancer; Multicatalytic endopeptidase complex; Rabbit; Monoclonal antibody; Subunit; Beta; Myeloproliferative syndrome; Restoration; Dicotyledones; Lymphoproliferative syndrome; Detection limit; Angiospermae; Chronic myelocytic leukemia; Development; Multienzyme complex; Enzyme; Acute; Use; Malignant hemopathy; Lagomorpha; Lymphoma; Chenopodiaceae; Peptidases; Vertebrata; Chronic; Mammalia; Hydrolases; Spermatophyta; Detection; ELISA assay; Acute myelocytic leukemia

Because quantification of the 20S proteasome by functional activity measurements is difficult and inaccurate, we have developed an indirect sandwich enzyme-linked immunosorbent assays (ELISA) for quantification of the 20S proteasome in human plasma. This sandwich ELISA uses a combination of a monocl...