Characterization of the Urease Operon of Brucella abortus and Assessment of Its Role in Virulence of the Bacterium
Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly understood. We isolated several Tn5 insertion mutants deficient in urease activity from Brucella abortus strain 2308. The mutations of most of these m...
Saved in:
Published in | Infection and Immunity Vol. 75; no. 2; pp. 774 - 780 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Society for Microbiology
01.02.2007
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly understood. We isolated several Tn5 insertion mutants deficient in urease activity from Brucella abortus strain 2308. The mutations of most of these mutants mapped to a 5.7-kbp DNA region essential for urease activity. Sequencing of this region, designated ure1, revealed the presence of seven open reading frames corresponding to the urease structural proteins (UreA, UreB, and UreC) and the accessory proteins (UreD, UreE, UreF, and UreG). In addition to the urease genes, another gene (cobT) was identified, and inactivation of this gene affected urease activity in BRUCELLA: Subsequent analysis of the previously described sequences of the genomes of Brucella spp. revealed the presence of a second urease cluster, ure2, in all them. The ure2 locus was apparently inactive in B. abortus 2308. Urease-deficient mutants were used to evaluate the role of urease in Brucella pathogenesis. The urease-producing strains were found to be resistant in vitro to strong acid conditions in the presence of urea, while urease-negative mutants were susceptible to acid treatment. Similarly, the urease-negative mutants were killed more efficiently than the urease-producing strains during transit through the stomach. These results suggested that urease protects brucellae during their passage through the stomach when the bacteria are acquired by the oral route, which is the major route of infection in human brucellosis. |
---|---|
AbstractList | Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly understood. We isolated several Tn5 insertion mutants deficient in urease activity from Brucella abortus strain 2308. The mutations of most of these mutants mapped to a 5.7-kbp DNA region essential for urease activity. Sequencing of this region, designated ure1, revealed the presence of seven open reading frames corresponding to the urease structural proteins (UreA, UreB, and UreC) and the accessory proteins (UreD, UreE, UreF, and UreG). In addition to the urease genes, another gene (cobT) was identified, and inactivation of this gene affected urease activity in BRUCELLA: Subsequent analysis of the previously described sequences of the genomes of Brucella spp. revealed the presence of a second urease cluster, ure2, in all them. The ure2 locus was apparently inactive in B. abortus 2308. Urease-deficient mutants were used to evaluate the role of urease in Brucella pathogenesis. The urease-producing strains were found to be resistant in vitro to strong acid conditions in the presence of urea, while urease-negative mutants were susceptible to acid treatment. Similarly, the urease-negative mutants were killed more efficiently than the urease-producing strains during transit through the stomach. These results suggested that urease protects brucellae during their passage through the stomach when the bacteria are acquired by the oral route, which is the major route of infection in human brucellosis. Classifications Services IAI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue IAI About IAI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy Connect to IAI IAI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0019-9567 Online ISSN: 1098-5522 Copyright © 2014 by the American Society for Microbiology. For an alternate route to IAI .asm.org, visit: IAI Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly understood. We isolated several Tn 5 insertion mutants deficient in urease activity from Brucella abortus strain 2308. The mutations of most of these mutants mapped to a 5.7-kbp DNA region essential for urease activity. Sequencing of this region, designated ure1 , revealed the presence of seven open reading frames corresponding to the urease structural proteins (UreA, UreB, and UreC) and the accessory proteins (UreD, UreE, UreF, and UreG). In addition to the urease genes, another gene ( cobT ) was identified, and inactivation of this gene affected urease activity in Brucella . Subsequent analysis of the previously described sequences of the genomes of Brucella spp. revealed the presence of a second urease cluster, ure2 , in all them. The ure2 locus was apparently inactive in B. abortus 2308. Urease-deficient mutants were used to evaluate the role of urease in Brucella pathogenesis. The urease-producing strains were found to be resistant in vitro to strong acid conditions in the presence of urea, while urease-negative mutants were susceptible to acid treatment. Similarly, the urease-negative mutants were killed more efficiently than the urease-producing strains during transit through the stomach. These results suggested that urease protects brucellae during their passage through the stomach when the bacteria are acquired by the oral route, which is the major route of infection in human brucellosis. ABSTRACT Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly understood. We isolated several Tn 5 insertion mutants deficient in urease activity from Brucella abortus strain 2308. The mutations of most of these mutants mapped to a 5.7-kbp DNA region essential for urease activity. Sequencing of this region, designated ure1 , revealed the presence of seven open reading frames corresponding to the urease structural proteins (UreA, UreB, and UreC) and the accessory proteins (UreD, UreE, UreF, and UreG). In addition to the urease genes, another gene ( cobT ) was identified, and inactivation of this gene affected urease activity in Brucella . Subsequent analysis of the previously described sequences of the genomes of Brucella spp. revealed the presence of a second urease cluster, ure2 , in all them. The ure2 locus was apparently inactive in B. abortus 2308. Urease-deficient mutants were used to evaluate the role of urease in Brucella pathogenesis. The urease-producing strains were found to be resistant in vitro to strong acid conditions in the presence of urea, while urease-negative mutants were susceptible to acid treatment. Similarly, the urease-negative mutants were killed more efficiently than the urease-producing strains during transit through the stomach. These results suggested that urease protects brucellae during their passage through the stomach when the bacteria are acquired by the oral route, which is the major route of infection in human brucellosis. |
Author | Rodríguez, María Cruz Agüero, Jesús García Lobo, Juan M Seoane, Asunción Sangari, Félix J |
AuthorAffiliation | Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, c/Cardenal Herrera Oria s/n, 39011 Santander, Spain, 1 Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Santander, Spain 2 |
AuthorAffiliation_xml | – name: Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, c/Cardenal Herrera Oria s/n, 39011 Santander, Spain, 1 Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Santander, Spain 2 |
Author_xml | – sequence: 1 fullname: Sangari, Félix J – sequence: 2 fullname: Seoane, Asunción – sequence: 3 fullname: Rodríguez, María Cruz – sequence: 4 fullname: Agüero, Jesús – sequence: 5 fullname: García Lobo, Juan M |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18473270$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/17101645$$D View this record in MEDLINE/PubMed |
BookMark | eNpVkc1v1DAQxS1URLeFG2fwBU6k-DNOLkjbFR8rVaoELFdr4ti7Rkm82AkI_nqcZqFwsjzz05s38y7Q2RAGi9BTSq4oZdXr7Xp7RSgToiDlA7SipK4KKRk7QytCaF3UslTn6CKlr_krhKgeoXOqKKGlkCsUNweIYEYb_S8YfRhwcHg8WLyLFpLFt0cbl-J1nIztOsDQhDhOCcPQ4nVKNqXeDuOMbMeEP4bOYj_gLz5OnR2M_SN4vUyZ-sfooYMu2Sen9xLt3r39vPlQ3Ny-327WN4WRXI6FqGvFm7rholFQipYb55QUpnYlraRjrDG5qDg3tHYOSqdaBpS3jeK2rSXhl-jNonucmt62JpuM0Olj9D3EnzqA1_93Bn_Q-_Bd04pVouJZ4OVJIIZvk02j7n26u8Fgw5Q0y6dWZTmDrxbQxJBStO7vEEr0HJLOIem7kDQpM_7sX2P38CmVDLw4AZAMdC7CYHy65yqhOFPzhnjhDn5_-OGj1ZB67fNiSmqmlRIZeb4gDoKGfcwyu0-MUE6IErM1_hvwm7FT |
CODEN | INFIBR |
CitedBy_id | crossref_primary_10_1016_j_micpath_2017_10_029 crossref_primary_10_2217_fmb_11_136 crossref_primary_10_1111_j_1462_2920_2008_01681_x crossref_primary_10_1073_pnas_1413209111 crossref_primary_10_1016_S0255_0857_21_02105_8 crossref_primary_10_1128_JB_00509_19 crossref_primary_10_3390_microorganisms10040813 crossref_primary_10_1371_journal_pone_0210874 crossref_primary_10_1016_j_jare_2018_05_010 crossref_primary_10_1038_icb_2016_5 crossref_primary_10_1111_tbed_13377 crossref_primary_10_3390_vaccines10030388 crossref_primary_10_1093_infdis_jis522 crossref_primary_10_3389_fmicb_2019_02751 crossref_primary_10_3389_fmicb_2021_794535 crossref_primary_10_1128_JB_00922_10 crossref_primary_10_1016_j_micpath_2011_07_006 crossref_primary_10_1007_s12088_015_0545_5 crossref_primary_10_1021_cr900010n crossref_primary_10_1089_fpd_2022_0063 crossref_primary_10_1093_nar_gkm377 crossref_primary_10_1186_1471_2180_7_57 crossref_primary_10_1128_IAI_00417_09 crossref_primary_10_3389_fmicb_2017_02236 crossref_primary_10_1111_1574_6976_12076 crossref_primary_10_1016_j_toxicon_2015_11_020 crossref_primary_10_1016_j_vetmic_2019_02_005 crossref_primary_10_3390_pathogens12111274 crossref_primary_10_1007_s00203_021_02664_1 crossref_primary_10_1099_mgen_0_000786 crossref_primary_10_1186_2045_3701_1_17 crossref_primary_10_1016_j_meegid_2020_104635 crossref_primary_10_1155_2011_518323 crossref_primary_10_3389_fimmu_2019_01436 crossref_primary_10_3390_genes3020191 crossref_primary_10_3389_fimmu_2019_01759 crossref_primary_10_1186_1471_2164_14_459 crossref_primary_10_3389_fimmu_2021_679560 crossref_primary_10_1016_j_vaccine_2016_08_076 crossref_primary_10_1074_jbc_M113_459305 crossref_primary_10_1128_JB_00105_18 crossref_primary_10_1016_j_tim_2012_03_001 crossref_primary_10_21307_pjm_2018_029 crossref_primary_10_1016_j_cimid_2023_102099 crossref_primary_10_1017_S1466252312000047 crossref_primary_10_4236_aim_2014_47046 crossref_primary_10_3934_microbiol_2021008 crossref_primary_10_1128_IAI_00224_13 crossref_primary_10_1007_s42770_018_0006_5 crossref_primary_10_1186_1471_2180_11_176 crossref_primary_10_1371_journal_pntd_0009171 crossref_primary_10_3109_1040841X_2014_962480 crossref_primary_10_1111_lam_12237 crossref_primary_10_3390_genes11091016 crossref_primary_10_1038_srep39197 crossref_primary_10_3390_inorganics7070080 crossref_primary_10_3389_fmicb_2015_01437 crossref_primary_10_1007_s00430_009_0123_8 crossref_primary_10_3389_fmicb_2020_598797 crossref_primary_10_1128_IAI_00210_12 crossref_primary_10_1007_s00430_011_0190_5 crossref_primary_10_1016_j_ejbt_2020_10_002 crossref_primary_10_1016_j_ram_2023_11_005 crossref_primary_10_1186_1471_2180_11_183 crossref_primary_10_1016_j_ejmech_2014_01_001 crossref_primary_10_3390_pathogens9050328 crossref_primary_10_1016_j_ccr_2021_214190 crossref_primary_10_1016_j_micpath_2019_01_010 crossref_primary_10_1371_journal_pone_0005519 crossref_primary_10_1016_j_vetmic_2007_11_023 crossref_primary_10_1080_21505594_2015_1038015 crossref_primary_10_1089_fpd_2022_0001 crossref_primary_10_1111_j_1745_4565_2011_00325_x crossref_primary_10_1099_mic_0_000702 crossref_primary_10_1093_femsre_fuac037 crossref_primary_10_3389_fcimb_2017_00186 crossref_primary_10_1186_1471_2180_8_121 crossref_primary_10_1080_21505594_2018_1511677 crossref_primary_10_1016_j_bioorg_2018_06_007 crossref_primary_10_1016_j_vaccine_2009_08_058 crossref_primary_10_1128_AEM_02928_14 crossref_primary_10_1128_MMBR_00021_19 crossref_primary_10_1186_1471_2180_14_42 crossref_primary_10_1146_annurev_micro_090110_102905 crossref_primary_10_4155_fmc_2023_0168 crossref_primary_10_1039_C4MT00245H |
Cites_doi | 10.1073/pnas.221575398 10.1152/ajpcell.1982.242.5.C333 10.1016/0003-2697(76)90527-3 10.1016/j.resmic.2005.06.006 10.1111/j.1365-2958.1993.tb01220.x 10.1016/S0034-5288(18)31838-1 10.1073/pnas.192319099 10.1128/JB.183.4.1242-1247.2001 10.1128/jb.176.12.3568-3575.1994 10.1093/nar/27.22.4305 10.1016/0378-1119(93)90611-6 10.1016/0016-5085(90)90957-3 10.1128/IAI.67.8.4041-4047.1999 10.1111/j.1365-2958.1994.tb01306.x 10.1128/iai.59.6.2036-2042.1991 10.1111/j.1365-2958.1996.tb02533.x 10.1038/nbt1183-784 10.1016/S0378-1135(02)00231-6 10.1128/iai.61.1.364-367.1993 10.1128/JB.187.20.7150-7154.2005 10.1093/infdis/170.5.1216 10.1128/IAI.74.2.1148-1155.2006 10.3109/10408419009105726 10.1128/jb.178.22.6487-6495.1996 10.1146/annurev.micro.57.030502.090820 10.1128/mr.59.3.451-480.1995 10.1128/iai.63.10.3790-3795.1995 10.1111/j.1939-1676.2005.tb02786.x 10.1111/j.1472-765X.1989.tb00262.x 10.1099/00222615-13-4-507 10.1128/jb.175.11.3253-3258.1993 10.1128/iai.61.6.2498-2504.1993 10.1128/JB.187.8.2715-2726.2005 10.1128/JB.183.2.426-434.2001 10.1128/IAI.66.11.5060-5066.1998 10.1128/iai.61.7.2748-2754.1993 10.1016/0882-4010(91)90040-H 10.1128/IAI.73.12.8353-8361.2005 |
ContentType | Journal Article |
Copyright | 2007 INIST-CNRS Copyright © 2007, American Society for Microbiology 2007 |
Copyright_xml | – notice: 2007 INIST-CNRS – notice: Copyright © 2007, American Society for Microbiology 2007 |
DBID | FBQ IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QL 7T5 8FD C1K FR3 H94 P64 RC3 5PM |
DOI | 10.1128/IAI.01244-06 |
DatabaseName | AGRIS Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Bacteriology Abstracts (Microbiology B) Immunology Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Biotechnology and BioEngineering Abstracts Genetics Abstracts PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Genetics Abstracts Technology Research Database Bacteriology Abstracts (Microbiology B) AIDS and Cancer Research Abstracts Immunology Abstracts Engineering Research Database Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management |
DatabaseTitleList | Genetics Abstracts MEDLINE CrossRef |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Biology |
EISSN | 1098-5522 |
EndPage | 780 |
ExternalDocumentID | 10_1128_IAI_01244_06 17101645 18473270 iai_75_2_774 US201300741128 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- -DZ -~X .55 .GJ 0R~ 18M 29I 2WC 39C 3O- 4.4 41~ 53G 5GY 5RE 5VS 85S ABOCM ACGFO ADBBV AENEX AFMIJ AGCDD AI. ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BTFSW C1A CS3 D0S DIK DU5 E3Z EBS EJD F5P FBQ FRP GX1 HYE HZ~ H~9 IH2 J5H KQ8 L7B MVM NEJ O9- OHT OK1 P2P RHF RHI RNS RPM RSF SJN TR2 TWZ UCJ UPT VH1 VQA W2D W8F WH7 WHG WOQ X7M XFK Y6R ZA5 ZGI ZXP ~KM 08R AAPBV AAUGY H13 IQODW CGR CUY CVF ECM EIF NPM AAYXX AGVNZ CITATION 7QL 7T5 8FD C1K FR3 H94 P64 RC3 5PM |
ID | FETCH-LOGICAL-c535t-49973b9b34b7a64d3cff754c9f6185f22bc4d3733c19ffa6f7d2a13db73ed9503 |
IEDL.DBID | RPM |
ISSN | 0019-9567 |
IngestDate | Tue Sep 17 21:19:36 EDT 2024 Sat Aug 17 01:00:21 EDT 2024 Thu Sep 12 18:18:55 EDT 2024 Thu May 23 23:11:33 EDT 2024 Sun Oct 22 16:07:04 EDT 2023 Wed May 18 15:26:59 EDT 2016 Wed Dec 27 19:14:59 EST 2023 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Keywords | Brucellaceae Microbiology Enzyme Operon Virulence Bacteria Hydrolases Urease Brucella abortus Immunity Brucella melitensis |
Language | English |
License | CC BY 4.0 |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c535t-49973b9b34b7a64d3cff754c9f6185f22bc4d3733c19ffa6f7d2a13db73ed9503 |
Notes | http://iai.asm.org/ ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Editor: A. Camilli Corresponding author. Mailing address: Departamento de Biología Molecular, Universidad de Cantabria, C/Cardenal Herrera Oria s/n, 39011 Santander, Spain. Phone: 34-942201948. Fax: 34-942201945. E-mail: jmglobo@unican.es. |
OpenAccessLink | https://doi.org/10.1128/iai.01244-06 |
PMID | 17101645 |
PQID | 20987663 |
PQPubID | 23462 |
PageCount | 7 |
ParticipantIDs | pubmed_primary_17101645 pubmedcentral_primary_oai_pubmedcentral_nih_gov_1828483 crossref_primary_10_1128_IAI_01244_06 fao_agris_US201300741128 proquest_miscellaneous_20987663 pascalfrancis_primary_18473270 highwire_asm_iai_75_2_774 |
PublicationCentury | 2000 |
PublicationDate | 2007-02-01 |
PublicationDateYYYYMMDD | 2007-02-01 |
PublicationDate_xml | – month: 02 year: 2007 text: 2007-02-01 day: 01 |
PublicationDecade | 2000 |
PublicationPlace | Washington, DC |
PublicationPlace_xml | – name: Washington, DC – name: United States |
PublicationTitle | Infection and Immunity |
PublicationTitleAlternate | Infect Immun |
PublicationYear | 2007 |
Publisher | American Society for Microbiology |
Publisher_xml | – name: American Society for Microbiology |
References | 8514376 - Infect Immun. 1993 Jul;61(7):2748-54 16428763 - Infect Immun. 2006 Feb;74(2):1148-55 16199586 - J Bacteriol. 2005 Oct;187(20):7150-4 10536136 - Nucleic Acids Res. 1999 Nov 15;27(22):4305-13 11756688 - Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):443-8 7558281 - Infect Immun. 1995 Oct;63(10):3790-5 9784504 - Infect Immun. 1998 Nov;66(11):5060-6 8501029 - J Bacteriol. 1993 Jun;175(11):3253-8 2379775 - Gastroenterology. 1990 Sep;99(3):697-702 8068328 - Biotechniques. 1994 May;16(5):800-2 10417172 - Infect Immun. 1999 Aug;67(8):4041-7 942051 - Anal Biochem. 1976 May 7;72:248-54 4001565 - Res Vet Sci. 1985 Mar;38(2):252-3 12730324 - Annu Rev Microbiol. 2003;57:155-76 7565414 - Microbiol Rev. 1995 Sep;59(3):451-80 16299333 - Infect Immun. 2005 Dec;73(12):8353-61 8809745 - Mol Microbiol. 1996 Jun;20(5):919-25 8418063 - Infect Immun. 1993 Jan;61(1):364-7 10935879 - Can J Vet Res. 2000 Jul;64(3):145-50 15805518 - J Bacteriol. 2005 Apr;187(8):2715-26 8206834 - J Bacteriol. 1994 Jun;176(12):3568-75 7001026 - J Med Microbiol. 1980 Nov;13(4):507-12 7891556 - Mol Microbiol. 1994 Nov;14(4):681-9 7065255 - Am J Physiol. 1982 Apr;242(4):G333-6 16355689 - J Vet Intern Med. 2005 Nov-Dec;19(6):905-13 11157936 - J Bacteriol. 2001 Feb;183(4):1242-7 12271122 - Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13148-53 2154232 - Crit Rev Microbiol. 1990;17(3):209-30 2037363 - Infect Immun. 1991 Jun;59(6):2036-42 8932305 - J Bacteriol. 1996 Nov;178(22):6487-95 11133934 - J Bacteriol. 2001 Jan;183(2):426-34 8500886 - Infect Immun. 1993 Jun;61(6):2498-504 16139482 - Res Microbiol. 2006 Mar;157(2):184-93 7934918 - Mol Microbiol. 1993 Sep;9(5):907-13 7963716 - J Infect Dis. 1994 Nov;170(5):1216-23 12414167 - Vet Microbiol. 2002 Dec 20;90(1-4):497-519 1665539 - Microb Pathog. 1991 Dec;11(6):443-6 8486283 - Gene. 1993 May 15;127(1):15-21 e_1_3_2_26_2 e_1_3_2_27_2 e_1_3_2_28_2 e_1_3_2_29_2 (e_1_3_2_43_2) 2000 e_1_3_2_41_2 e_1_3_2_40_2 e_1_3_2_21_2 e_1_3_2_42_2 e_1_3_2_22_2 e_1_3_2_23_2 e_1_3_2_44_2 e_1_3_2_24_2 e_1_3_2_25_2 (e_1_3_2_6_2) 2000; 64 (e_1_3_2_20_2) 1994; 16 e_1_3_2_9_2 e_1_3_2_15_2 e_1_3_2_38_2 e_1_3_2_8_2 e_1_3_2_16_2 e_1_3_2_37_2 e_1_3_2_7_2 e_1_3_2_17_2 e_1_3_2_18_2 e_1_3_2_39_2 e_1_3_2_19_2 e_1_3_2_30_2 e_1_3_2_32_2 e_1_3_2_10_2 e_1_3_2_31_2 e_1_3_2_5_2 e_1_3_2_11_2 e_1_3_2_34_2 e_1_3_2_4_2 e_1_3_2_12_2 e_1_3_2_33_2 e_1_3_2_3_2 e_1_3_2_13_2 e_1_3_2_36_2 e_1_3_2_2_2 e_1_3_2_14_2 e_1_3_2_35_2 |
References_xml | – ident: e_1_3_2_13_2 doi: 10.1073/pnas.221575398 – ident: e_1_3_2_27_2 doi: 10.1152/ajpcell.1982.242.5.C333 – volume: 16 start-page: 800 year: 1994 ident: e_1_3_2_20_2 publication-title: BioTechniques – ident: e_1_3_2_7_2 doi: 10.1016/0003-2697(76)90527-3 – ident: e_1_3_2_23_2 doi: 10.1016/j.resmic.2005.06.006 – ident: e_1_3_2_10_2 doi: 10.1111/j.1365-2958.1993.tb01220.x – ident: e_1_3_2_11_2 doi: 10.1016/S0034-5288(18)31838-1 – ident: e_1_3_2_28_2 doi: 10.1073/pnas.192319099 – ident: e_1_3_2_5_2 doi: 10.1128/JB.183.4.1242-1247.2001 – ident: e_1_3_2_41_2 doi: 10.1128/jb.176.12.3568-3575.1994 – ident: e_1_3_2_3_2 doi: 10.1093/nar/27.22.4305 – ident: e_1_3_2_32_2 doi: 10.1016/0378-1119(93)90611-6 – ident: e_1_3_2_24_2 doi: 10.1016/0016-5085(90)90957-3 – ident: e_1_3_2_31_2 doi: 10.1128/IAI.67.8.4041-4047.1999 – ident: e_1_3_2_15_2 doi: 10.1111/j.1365-2958.1994.tb01306.x – ident: e_1_3_2_25_2 doi: 10.1128/iai.59.6.2036-2042.1991 – ident: e_1_3_2_34_2 – ident: e_1_3_2_29_2 doi: 10.1111/j.1365-2958.1996.tb02533.x – ident: e_1_3_2_37_2 doi: 10.1038/nbt1183-784 – ident: e_1_3_2_2_2 doi: 10.1016/S0378-1135(02)00231-6 – volume: 64 start-page: 145 year: 2000 ident: e_1_3_2_6_2 publication-title: Can. J. Vet. Res. – ident: e_1_3_2_14_2 doi: 10.1128/iai.61.1.364-367.1993 – ident: e_1_3_2_19_2 doi: 10.1128/JB.187.20.7150-7154.2005 – ident: e_1_3_2_9_2 doi: 10.1093/infdis/170.5.1216 – ident: e_1_3_2_4_2 doi: 10.1128/IAI.74.2.1148-1155.2006 – ident: e_1_3_2_22_2 – ident: e_1_3_2_40_2 doi: 10.3109/10408419009105726 – ident: e_1_3_2_44_2 doi: 10.1128/jb.178.22.6487-6495.1996 – ident: e_1_3_2_33_2 doi: 10.1146/annurev.micro.57.030502.090820 – ident: e_1_3_2_26_2 doi: 10.1128/mr.59.3.451-480.1995 – start-page: 2053 year: 2000 ident: e_1_3_2_43_2 publication-title: Mandell, Douglas, and Bennett's principles and practice of infectious diseases – ident: e_1_3_2_12_2 doi: 10.1128/iai.63.10.3790-3795.1995 – ident: e_1_3_2_42_2 doi: 10.1111/j.1939-1676.2005.tb02786.x – ident: e_1_3_2_30_2 doi: 10.1111/j.1472-765X.1989.tb00262.x – ident: e_1_3_2_36_2 doi: 10.1099/00222615-13-4-507 – ident: e_1_3_2_39_2 doi: 10.1128/jb.175.11.3253-3258.1993 – ident: e_1_3_2_38_2 doi: 10.1128/iai.61.6.2498-2504.1993 – ident: e_1_3_2_16_2 doi: 10.1128/JB.187.8.2715-2726.2005 – ident: e_1_3_2_18_2 doi: 10.1128/JB.183.2.426-434.2001 – ident: e_1_3_2_21_2 doi: 10.1128/IAI.66.11.5060-5066.1998 – ident: e_1_3_2_17_2 doi: 10.1128/iai.61.7.2748-2754.1993 – ident: e_1_3_2_35_2 doi: 10.1016/0882-4010(91)90040-H – ident: e_1_3_2_8_2 doi: 10.1128/IAI.73.12.8353-8361.2005 |
SSID | ssj0014448 |
Score | 2.2499251 |
Snippet | Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly... Classifications Services IAI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit... ABSTRACT Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly... Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly... |
SourceID | pubmedcentral proquest crossref pubmed pascalfrancis highwire fao |
SourceType | Open Access Repository Aggregation Database Index Database Publisher |
StartPage | 774 |
SubjectTerms | Acids - pharmacology Animals Anti-Bacterial Agents - pharmacology Bacteria Bacteriology Biological and medical sciences Brucella abortus Brucella abortus - drug effects Brucella abortus - enzymology Brucella abortus - genetics Brucella abortus - pathogenicity Brucellosis - microbiology Colony Count, Microbial Computational Biology Disease Models, Animal DNA Transposable Elements DNA, Bacterial - chemistry DNA, Bacterial - genetics Female Fundamental and applied biological sciences. Psychology Gene Deletion Mice Mice, Inbred BALB C Microbial Viability Microbiology Miscellaneous Molecular Pathogenesis Molecular Sequence Data Mutagenesis, Insertional Open Reading Frames Operon Sequence Analysis, DNA Sequence Homology, Amino Acid Stomach - microbiology Urea - metabolism Urease - genetics Urease - metabolism Virulence Factors - genetics Virulence Factors - metabolism |
Title | Characterization of the Urease Operon of Brucella abortus and Assessment of Its Role in Virulence of the Bacterium |
URI | http://iai.asm.org/content/75/2/774.abstract https://www.ncbi.nlm.nih.gov/pubmed/17101645 https://search.proquest.com/docview/20987663 https://pubmed.ncbi.nlm.nih.gov/PMC1828483 |
Volume | 75 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwEB5tVwJxQbA8NjwWH-CYNrVjOzmWitUWqYCAor1ZjhNDpG2yapID_56xk3RbxIlrbMeKv7FnJv5mBuCtSFgWCZ2iW0KLMM6zNNRG5GFGmUZ5iuzcl_NZfxJXm_jjNb8-AT7GwnjSvsnKaXWznVblL8-tvN2a2cgTm31ZL9EmTuKEzSYwkYyNLvpwdRDH8XD8piEa_3Jku9NktlqsppFTaGHkSxdJ57q6QKYDlTSxuj5IFuy4krrB5bJ9nYt_GaJ_8ykPFNTlI3g4WJZk0X_BYzgpqjO419ea_H0G99fDLfoT2C33WZr7IExSW4KGINk4hnpBPrvk4f6hRx4FhThRabuG6Coni30yT9dl1Tbka31TkLIiP8pd54OYxhe-72fptk9hc_nh-_IqHGovhIYz3oboCEmWpRmLM6lFnDNjreSxSa1ADW8pzQw-xNU389RaLazMqZ67ZM2syFMesWdwWtVVcQ4EG2gkXIhsgahZkSWGcznX6HxzKucmgHfj8qvbPsWG8q4JTRQipjxiKhIBnCM2Sv_E009tvlF35-oMIuwXQDACpnSzVaUuleSKKjRuA7g4QvBuDlTMjMoogDcjpAr3ll_Wqqi7RtEoRWUhWADPe4Dvxg6iE4A8gn7fwWXtPm5BYfbZuwfhffHfI1_Cg_4Hs-PUvILTdtcVr9EyarMLvxP-AAvBClk |
link.rule.ids | 230,315,733,786,790,891,27957,27958,53827,53829 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lc9MwEN5py0C58CiPmkerAxydOJJt2ceQoZNAUxhoOr1pJNkCTxunk9gH-PWsZDtNOlzgKsn2WP6k3bW-_RbgXZwwFcQyxbCE5n6YqdSXOs58RZlEPAVm4Mr5TM_i8Sz8dBld7kDU5cI40r5WRa-8nvfK4qfjVt7Mdb_jifW_TkfoEydhwvq7cA_XK-VdkN4eHoRh2G7AqY_uP-_47jTpT4aTXmBNmh-44kXcBq82lWnDKO0audiQC7ZsSbnCCTNNpYu_uaJ3GZUbJurkMVx0L9cwU656daV6-vcd3cd_fvsn8Kh1Wsmw6X4KO3l5APebMpa_DuDBtD2gfwbL0VoAusnvJAtD0MckM0t-z8kXq0vuGh2oEIPEorCqV0SWGRmudULtkEm1It8W1zkpSnJRLGuXH9Xd8EPzlHr-HGYnH89HY78t6-DriEWVjzEWZypVLFRcxmHGtDE8CnVqYnQeDKVKYyNnTA9SY2RseEblwOpAszxLo4C9gL1yUeaHQLCDBrHNvs1xSkysEh1FfCAxro8oH2gP3nffVdw06h3CRT00EQgF4aAggtiDQ_zoQv7AjVXMvlN7nGt9LRzngdchQcjVXBSyEDwSVKDf7MHRFjRun4E2n1EeeHDcYUXgsnXTWuaLeiVokKIdipkHLxvk3F7bYtIDvoWp9QArCL7dg0hxwuAtMl7995XHsD8-n56K08nZ59fwsPmPbak7b2CvWtb5W3TAKnXkltsfOcEsUw |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nc9MwEN2hZehw4aNQaj5aHeDoxJFsyz6GQKYBUjpAmA4XjSRb4KFxMol9gF_PSrbTpMOpV0uyxtKTdtd6egvwOk6YCmKZYlhCcz_MVOpLHWe-okwingIzcOl8pufx2Sz8cBldbqX6cqR9rYpeeTXvlcUvx61cznW_44n1L6Yj9ImTMGH9ZWb6e3AX1yxNu0C9PUAIw7DdhFMfQwDecd5p0p8MJ73AmjU_cAmMuA1g7XWmLcO0Z-RiSzLYMiblGgfNNNku_ueO3mRVbpmp8UP40X1gw0753asr1dN_b2g_3moEHsGD1nklw6bKY7iTl4dwr0ln-ecQDqbtQf0TWI02QtDNPU-yMAR9TTKzJPicfLb65O6hAxdikVg0VvWayDIjw41eqK0yqdbky-IqJ0VJvher2t2T6l74tumlnj-F2fj9t9GZ36Z38HXEosrHWIszlSoWKi7jMGPaGB6FOjUxOhGGUqXxIWdMD1JjZGx4RuXA6kGzPEujgB3Bfrko82MgWECD2N7CzXFYTKwSHUV8IDG-jygfaA_edHMrlo2Kh3DRD00EwkE4OIgg9uAYJ17In7jBitlXao91rc-F9TzwOjQIuZ6LQhaCR4IK9J89ONmBx3UfaPsZ5YEHpx1eBC5fN6xlvqjXggYp2qOYefCsQc912xaXHvAdXG0qWGHw3RJEixMIb9Hx_NYtT-Hg4t1YfJqcf3wB95vf2ZbB8xL2q1Wdv0I_rFInbsX9A6zOLtM |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Characterization+of+the+Urease+Operon+of+Brucella+abortus+and+Assessment+of+Its+Role+in+Virulence+of+the+Bacterium&rft.jtitle=Infection+and+immunity&rft.au=Sangari%2C+Fe%CC%81lix+J.&rft.au=Seoane%2C+Asuncio%CC%81n&rft.au=Rodri%CC%81guez%2C+Mari%CC%81a+Cruz&rft.au=Agu%CC%88ero%2C+Jesu%CC%81s&rft.date=2007-02-01&rft.issn=0019-9567&rft.eissn=1098-5522&rft.volume=75&rft.issue=2&rft.spage=774&rft.epage=780&rft_id=info:doi/10.1128%2FIAI.01244-06&rft.externalDBID=n%2Fa&rft.externalDocID=10_1128_IAI_01244_06 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0019-9567&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0019-9567&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0019-9567&client=summon |