Propagation of Rhinovirus C in Differentiated Immortalized Human Airway HBEC3-KT Epithelial Cells

• Published in: Viruses Vol. 11; no. 3; p. 216
• Main Authors: Nakauchi, Mina, Nagata, Noriyo, Takayama, Ikuyo, Saito, Shinji, Kubo, Hideyuki, Kaida, Atsushi, Oba, Kunihiro, Odagiri, Takato, Kageyama, Tsutomu
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.03.2019; MDPI
• Subjects: air–liquid–interface culture; Cell Count; Cell culture; Cell Differentiation; Cell Line, Transformed; Cilia; Clinical isolates; DNA polymerase; Enterovirus - genetics; Enterovirus - growth & development; Enterovirus - physiology; Epithelial cells; Epithelial Cells - virology; Genomes; Goblet cells; Humans; immortalized cells; Propagation; Respiratory System - cytology; Respiratory tract; Rhinovirus; rhinovirus C; Virus Cultivation; Viruses; VP1 protein; United States > US; immortalized cells; air–liquid–interface culture; rhinovirus C; clinical isolates

Rhinoviruses (RVs) are classified into three species: RV-A, B, and C. Unlike RV-A and -B, RV-C cannot be propagated using standard cell culture systems. In order to isolate RV-Cs from clinical specimens and gain a better understanding of their biological properties and pathogenesis, we established a...