Specific Detection of Bifidobacterium Strains in a Pharmaceutical Probiotic Product and in Human Feces by Polymerase Chain Reaction

• Published in: Systematic and applied microbiology Vol. 23; no. 3; pp. 391 - 399
• Main Authors: Brigidi, Patrizia, Vitali, Beatrice, Swennen, Erwin, Altomare, Luigi, Rossi, Maddalena, Matteuzzi, Diego
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.10.2000; Elsevier
• Subjects: 16S-23S rDNA; Bacterial Typing Techniques; Bacteriology; Bifidobacterium; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Bifidobacterium infantis; Bifidobacterium longum; Biological and medical sciences; Colitis, Ulcerative - microbiology; DNA Primers; DNA, Ribosomal; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Genetics; Human feces; Humans; Identification; Industrial Microbiology; Microbiology; Molecular Sequence Data; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; PCR primers; Polymerase Chain Reaction - methods; Pouchitis - microbiology; Probiotics; RNA, Ribosomal, 16S; RNA, Ribosomal, 23S; Sequence Analysis, DNA; Species Specificity; PCR primers; Probiotics; Human feces; Identification; 16S-23S rDNA; Bifidobacterium; Human; Probiotic; Actinomycetaceae; New product; Ribosomal DNA; Primer; Bifidobacterium longum; Strain specificity; Strain; Actinomycetales; Polymerase chain reaction; 16S-RNA; Bacteria; Actinomycetes; Feces; Detection; By product
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/S0723-2020(00)80070-3

For PCR specific detection of the strains Bifidobacterium longum Y 10, B. infantis Y 1 and B. breve Y 8 used in a new probiotic product (VSL-3), strains-specific rDNA primers have been developed. Spacer regions between the 16S and 23S rRNA genes (ITS) of the three strains were amplified by PCR with...