Rabies virus glycoprotein serology ELISA for measurement of neutralizing antibodies in sera of vaccinated human subjects

• Published in: Vaccine Vol. 37; no. 41; pp. 6060 - 6067
• Main Authors: Zhao, Rongqing, Yu, Pengcheng, Shan, Yi, Thirumeni, Nagarajan, Li, Maohua, Lv, Yanli, Li, Jianli, Ren, Wenlin, Huang, Lisong, Wei, Jingshuang, Sun, Yufei, Zhu, Wuyang, Sun, Le
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 24.09.2019; Elsevier Limited
• Subjects: Allergy and Immunology; Antibiotics; Antibodies; Antigens; Assaying; blood serum; coatings; detection limit; diagnostic specificity; Diagnostic systems; Dilution; ELISA; Enzyme-linked immunosorbent assay; Glycoprotein (GP); Glycoproteins; Human subjects; humans; Immune response; Immune system; Immunity; Immunoglobulins; Lyssavirus; Mammalian cells; Medical research; monitoring; neutralizing antibodies; Protective coatings; Rabies; Rabies lyssavirus; Rabies Virus (RABV); Reproducibility; Sensitivity analysis; Serology; Standardization; Technicians; Vaccination; Vaccines; Virus neutralizing antibody (VNA); Viruses; United States > US; China; Virus neutralizing antibody (VNA); Rabies Virus (RABV); Glycoprotein (GP); ELISA

•First serology ELISA based on recombinant RABV GP for measurement of virus neutralizing antibodies.•Manufacturing and assay protocol are well-standardized with reliable production and test data.•The specificity and sensitivity of ELISA are 100% and 91.1% when benchmarked with the RFFIT method.•The...