Existing drugs as broad-spectrum and potent inhibitors for Zika virus by targeting NS2B-NS3 interaction

• Published in: Cell research Vol. 27; no. 8; pp. 1046 - 1064
• Main Authors: Li, Zhong, Brecher, Matthew, Deng, Yong-Qiang, Zhang, Jing, Sakamuru, Srilatha, Liu, Binbin, Huang, Ruili, Koetzner, Cheri A, Allen, Christina A, Jones, Susan A, Chen, Haiying, Zhang, Na-Na, Tian, Min, Gao, Fengshan, Lin, Qishan, Banavali, Nilesh, Zhou, Jia, Boles, Nathan, Xia, Menghang, Kramer, Laura D, Qin, Cheng-Feng, Li, Hongmin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.08.2017; Nature Publishing Group
• Subjects: 631/45/607/468; 631/80/86; 692/699/255/2514; 692/700/565/1436/2185; Animal models; Antiviral Agents - chemistry; Antiviral Agents - pharmacology; Biomedical and Life Sciences; Cell Biology; Cell Line; Cells (biology); Docking; Drug development; Drugs; Female; High-throughput screening; Humans; Inhibitors; Life Sciences; Molecular Docking Simulation; Neural stem cells; Neural Stem Cells - metabolism; Neural Stem Cells - virology; Niclosamide; Original; original-article; Outbreaks; Placenta; Placenta - metabolism; Placenta - virology; Pregnancy; RNA Helicases - chemistry; RNA Helicases - genetics; RNA Helicases - metabolism; Screening; Serine Endopeptidases - chemistry; Serine Endopeptidases - genetics; Serine Endopeptidases - metabolism; Vector-borne diseases; Viral Nonstructural Proteins - chemistry; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - metabolism; Viremia; Virus Replication - drug effects; Virus Replication - physiology; Viruses; Zika virus; Zika Virus - chemistry; Zika Virus - physiology; Zika Virus Infection - drug therapy; Zika Virus Infection - genetics; Zika Virus Infection - metabolism; protease; Zika virus; inhibitor; NS2B-NS3

Recent outbreaks of Zika virus （ZIKV） highlight an urgent need for therapeutics. The protease complex NS2B- NS3 plays essential roles during flaviviral polyprotein processing, and thus represents an attractive drug target. Here, we developed a split luciferase complementation-based high-throughput s...