Optogenetic modulation of guanine nucleotide exchange factors of Ras superfamily proteins directly controls cell shape and movement

• Published in: Frontiers in cell and developmental biology Vol. 11; p. 1195806
• Main Authors: Pal, Dhiman Sankar, Lin, Yiyan, Zhan, Huiwang, Banerjee, Tatsat, Kuhn, Jonathan, Providence, Stephenie, Devreotes, Peter N.
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 10.07.2023
• Subjects: actin cytoskeleton; cancer metastasis; Cell and Developmental Biology; development; growth factor signaling; immunity; optogenetics; actin cytoskeleton; development; neural networks; optogenetics; immunity; growth factor signaling; diabetes; cancer metastasis

In this article, we provide detailed protocols on using optogenetic dimerizers to acutely perturb activities of guanine nucleotide exchange factors (GEFs) specific to Ras, Rac or Rho small GTPases of the migratory networks in various mammalian and amoeba cell lines. These GEFs are crucial components...