A Practical and Efficient Cellular Substrate for the Generation of Induced Pluripotent Stem Cells from Adults: Blood‐Derived Endothelial Progenitor Cells

• Published in: Stem cells translational medicine Vol. 1; no. 12; pp. 855 - 865
• Main Authors: Geti, Imbisaat, Ormiston, Mark L., Rouhani, Foad, Toshner, Mark, Movassagh, Mehregan, Nichols, Jennifer, Mansfield, William, Southwood, Mark, Bradley, Allan, Rana, Amer Ahmed, Vallier, Ludovic, Morrell, Nicholas W.
• Format: Journal Article
• Language: English
• Published: England AlphaMed Press 01.12.2012; Oxford University Press
• Subjects: Adult; Adult Stem Cells - cytology; Adult Stem Cells - physiology; Animals; Bone marrow; Cell Culture Techniques - methods; Cell Differentiation - physiology; Cell Lineage - physiology; Cellular Reprogramming - physiology; Cloning; Data analysis; Data collection; Direct cell conversion; Efficiency; Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells; Endothelial cell; Endothelium, Vascular - cytology; Experiments; Fibroblasts; Genetic engineering; Genomes; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - physiology; Hemopoiesis; Humans; Induced pluripotent stem cells; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - physiology; Karyotypes; Karyotyping; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - physiology; Mice; Mice, SCID; Neoplasm Transplantation; Patients; Peripheral blood; Pluripotency; Progenitor cells; Pulmonary arteries; Regenerative Medicine - methods; Reprogramming; Stem Cell Transplantation - methods; Stem cells; Teratoma - pathology; Toxicology; Writing; United Kingdom > UK

This report describes the use of late‐outgrowth endothelial progenitor cells (L‐EPCs), as a cellular substrate for the generation of induced pluripotent stem cells (iPSCs). A protocol was developed that allows the reliable isolation of L‐EPCs from peripheral blood mononuclear cell preparations, incl...