Cloning, sequencing and expression of ribonucleotide reductase R2 from Trypanosoma brucei

• Published in: FEBS letters Vol. 414; no. 2; pp. 449 - 453
• Main Authors: Dormeyer, Matthias, Schöneck, Ralf, Dittmar, Gunnar A.G., Krauth-Siegel, R. Luise
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 08.09.1997
• Subjects: Amino Acid Sequence; Animals; Cloning, Molecular; DNA, Complementary; DNA, Protozoan - chemistry; Drug target; Escherichia coli; Escherichia coli - enzymology; Genes, Protozoan; Humans; Mice; Molecular Sequence Data; Plasmodium falciparum - enzymology; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Ribonucleotide reductase; Ribonucleotide Reductases - biosynthesis; Ribonucleotide Reductases - chemistry; Ribonucleotide Reductases - genetics; Sequence Alignment; Sequence Homology, Amino Acid; Trypanosoma brucei; Trypanosoma brucei brucei - enzymology; Trypanosoma brucei brucei - genetics; Trypanothione; RR; R2; nrd B; Ribonucleotide reductase; Drug target; IPTG; T.b; Trypanosoma brucei; Trypanothione; Ni-NTA; R1

Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR ( nrd B) has been cloned. It encodes a protein of 337 residues which shows about 60% identity with other eukaryotic R2 proteins. All residues which bind the iron center, the tyros...