Cloning, sequencing and expression of ribonucleotide reductase R2 from Trypanosoma brucei

Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR ( nrd B) has been cloned. It encodes a protein of 337 residues which shows about 60% identity with other eukaryotic R2 proteins. All residues which bind the iron center, the tyros...

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Published inFEBS letters Vol. 414; no. 2; pp. 449 - 453
Main Authors Dormeyer, Matthias, Schöneck, Ralf, Dittmar, Gunnar A.G., Krauth-Siegel, R. Luise
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 08.09.1997
Subjects
Amino Acid Sequence
Animals
Cloning, Molecular
DNA, Complementary
DNA, Protozoan - chemistry
Drug target
Escherichia coli
Escherichia coli - enzymology
Genes, Protozoan
Humans
Mice
Molecular Sequence Data
Plasmodium falciparum - enzymology
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Ribonucleotide reductase
Ribonucleotide Reductases - biosynthesis
Ribonucleotide Reductases - chemistry
Ribonucleotide Reductases - genetics
Sequence Alignment
Sequence Homology, Amino Acid
Trypanosoma brucei
Trypanosoma brucei brucei - enzymology
Trypanosoma brucei brucei - genetics
Trypanothione
RR
R2
nrd B
Ribonucleotide reductase
Drug target
IPTG
T.b
Trypanosoma brucei
Trypanothione
Ni-NTA
R1
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Abstract Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR ( nrd B) has been cloned. It encodes a protein of 337 residues which shows about 60% identity with other eukaryotic R2 proteins. All residues which bind the iron center, the tyrosyl radical or are supposed to participate in the radical transfer are conserved in the trypanosomal protein sequence. Overexpression of the gene in E. coli resulted in 2–5 mg pure R2 protein from 100 ml bacterial cell culture. Northern blot analysis revealed a transcript of 1.85 kb in bloodstream and procyclic forms of the parasite.
AbstractList Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR (nrd B) has been cloned. It encodes a protein of 337 residues which shows about 60% identity with other eukaryotic R2 proteins. All residues which bind the iron center, the tyrosyl radical or are supposed to participate in the radical transfer are conserved in the trypanosomal protein sequence. Overexpression of the gene in E. coli resulted in 2-5 mg pure R2 protein from 100 ml bacterial cell culture. Northern blot analysis revealed a transcript of 1.85 kb in bloodstream and procyclic forms of the parasite.
Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR ( nrd B) has been cloned. It encodes a protein of 337 residues which shows about 60% identity with other eukaryotic R2 proteins. All residues which bind the iron center, the tyrosyl radical or are supposed to participate in the radical transfer are conserved in the trypanosomal protein sequence. Overexpression of the gene in E. coli resulted in 2–5 mg pure R2 protein from 100 ml bacterial cell culture. Northern blot analysis revealed a transcript of 1.85 kb in bloodstream and procyclic forms of the parasite.
Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR ( nrd B ) has been cloned. It encodes a protein of 337 residues which shows about 60% identity with other eukaryotic R2 proteins. All residues which bind the iron center, the tyrosyl radical or are supposed to participate in the radical transfer are conserved in the trypanosomal protein sequence. Overexpression of the gene in E. coli resulted in 2–5 mg pure R2 protein from 100 ml bacterial cell culture. Northern blot analysis revealed a transcript of 1.85 kb in bloodstream and procyclic forms of the parasite.
Author Krauth-Siegel, R. Luise
Dittmar, Gunnar A.G.
Dormeyer, Matthias
Schöneck, Ralf
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Copyright 1997 Federation of European Biochemical Societies. All rights reserved.
FEBS Letters 414 (1997) 1873-3468 © 2015 Federation of European Biochemical Societies
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Keywords RR
R2
nrd B
Ribonucleotide reductase
Drug target
IPTG
T.b
Trypanosoma brucei
Trypanothione
Ni-NTA
R1
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Snippet Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR ( nrd B) has been cloned. It encodes a...
Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR (nrd B) has been cloned. It encodes a...
Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma brucei RR ( nrd B ) has been cloned. It encodes...
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StartPage 449
SubjectTerms Amino Acid Sequence
Animals
Cloning, Molecular
DNA, Complementary
DNA, Protozoan - chemistry
Drug target
Escherichia coli
Escherichia coli - enzymology
Genes, Protozoan
Humans
Mice
Molecular Sequence Data
Plasmodium falciparum - enzymology
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Ribonucleotide reductase
Ribonucleotide Reductases - biosynthesis
Ribonucleotide Reductases - chemistry
Ribonucleotide Reductases - genetics
Sequence Alignment
Sequence Homology, Amino Acid
Trypanosoma brucei
Trypanosoma brucei brucei - enzymology
Trypanosoma brucei brucei - genetics
Trypanothione
Title Cloning, sequencing and expression of ribonucleotide reductase R2 from Trypanosoma brucei
URI https://dx.doi.org/10.1016/S0014-5793(97)01036-3
https://onlinelibrary.wiley.com/doi/abs/10.1016%2FS0014-5793%2897%2901036-3
https://www.ncbi.nlm.nih.gov/pubmed/9315738
https://search.proquest.com/docview/16231796
https://search.proquest.com/docview/79320543
https://search.proquest.com/docview/876236690
Volume 414
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