Engineered aggregation inhibitor fusion for production of highly amyloidogenic human islet amyloid polypeptide - Mirecka, Ewa Agnieszka; Gremer, Lothar; Schiefer, Stephanie; Oesterhelt, Filipp; Stoldt, Matthias; Willbold, Dieter; Hoyer, Wolfgang (Journal of biotechnology, 2014)
Keywords: Agglomeration; Amino Acid Sequence - genetics; amyloid; atomic force microscopy; bacteriophages; Construction; Diabetes Mellitus, Type 2 - genetics; Escherichia coli; Escherichia coli - genetics; Gene Expression Regulation; Homogeneity; Human; Humans; hydrophobicity; Inhibitors; Islet amyloid polypeptide; Islet Amyloid Polypeptide - biosynthesis; Islet Amyloid Polypeptide - genetics; isotope labeling; Magnetic Resonance Spectroscopy; Microscopy, Atomic Force; noninsulin-dependent diabetes mellitus; nuclear magnetic resonance spectroscopy; Polypeptides; Protein aggregation; Protein Aggregation, Pathological - genetics; Protein engineering; Protein Folding; Protein Structure, Secondary; Proteins; Recombinant; Recombinant expression; β-Wrapin; β-Wrapin; Recombinant expression; Protein engineering; Protein aggregation; Islet amyloid polypeptide