Rapid detection assay of toxigenic Clostridioides difficile through PathOC RightGene, a novel high-speed polymerase chain reaction device

Nucleic acid amplification tests for diagnosing Clostridioides difficile infections (CDI) are improving to become faster and more accurate. This study aimed to evaluate the accuracy of rapid detection of toxigenic C. difficile using the novel high-speed polymerase chain reaction (PCR) device, PathOC...

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Published inDiagnostic Microbiology and Infectious Disease Vol. 99; no. 2; p. 115247
Main Authors Okanda, Takashi, Mitsutake, Hiroshi, Aso, Ryoko, Sekizawa, Ryuichi, Takemura, Hiromu, Matsumoto, Tetsuya, Nakamura, Shigeki
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.02.2021
Elsevier BV
Subjects
Bacterial Toxins
Binary toxin
Clostridioides difficile
Clostridioides difficile infection
Clostridium
Clostridium Infections
Feces
High-speed polymerase chain reaction
Humans
Immunoenzyme Techniques
Molecular Diagnostic Techniques
Nucleic acid amplification tests
Point-of-Care Testing
Polymerase Chain Reaction
Sensitivity and Specificity
Toxin B
Point-of-care testing
Clostridioides difficile infection
Binary toxin
Toxin B
High-speed polymerase chain reaction
Nucleic acid amplification tests
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Abstract Nucleic acid amplification tests for diagnosing Clostridioides difficile infections (CDI) are improving to become faster and more accurate. This study aimed to evaluate the accuracy of rapid detection of toxigenic C. difficile using the novel high-speed polymerase chain reaction (PCR) device, PathOC RightGene. These results were compared and evaluated with real-time PCR (qPCR) and enzyme immunoassays (EIA) kit. For this study, 102 C. difficile and 3 Clostridium species isolated from CDI patients were used. These C. difficile isolates were 85 toxigenic and 17 non-toxigenic strains. The results of qPCR served as a standard, and sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the PathOC Right Gene were 99.2%, 99.4%, 100%, 98.8%, and 99.3%, respectively. Turnaround time of qPCR and EIA was 85 and 30 minutes, whereas PathOC RightGene was only 25 minutes including DNA extraction. This novel high-speed PCR device detected toxigenic C. difficile rapidly and accurately.
AbstractList Nucleic acid amplification tests for diagnosing Clostridioides difficile infections (CDI) are improving to become faster and more accurate. This study aimed to evaluate the accuracy of rapid detection of toxigenic C. difficile using the novel high-speed polymerase chain reaction (PCR) device, PathOC RightGene. These results were compared and evaluated with real-time PCR (qPCR) and enzyme immunoassays (EIA) kit. For this study, 102 C. difficile and 3 Clostridium species isolated from CDI patients were used. These C. difficile isolates were 85 toxigenic and 17 non-toxigenic strains. The results of qPCR served as a standard, and sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the PathOC Right Gene were 99.2%, 99.4%, 100%, 98.8%, and 99.3%, respectively. Turnaround time of qPCR and EIA was 85 and 30 minutes, whereas PathOC RightGene was only 25 minutes including DNA extraction. This novel high-speed PCR device detected toxigenic C. difficile rapidly and accurately.
Nucleic acid amplification tests for diagnosing Clostridioides difficile infections (CDI) are improving to become faster and more accurate. This study aimed to evaluate the accuracy of rapid detection of toxigenic C. difficile using the novel high-speed polymerase chain reaction (PCR) device, PathOC RightGene. These results were compared and evaluated with real-time PCR (qPCR) and enzyme immunoassays (EIA) kit. For this study, 102 C. difficile and 3 Clostridium species isolated from CDI patients were used. These C. difficile isolates were 85 toxigenic and 17 non-toxigenic strains. The results of qPCR served as a standard, and sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the PathOC Right Gene were 99.2%, 99.4%, 100%, 98.8%, and 99.3%, respectively. Turnaround time of qPCR and EIA was 85 and 30 minutes, whereas PathOC RightGene was only 25 minutes including DNA extraction. This novel high-speed PCR device detected toxigenic C. difficile rapidly and accurately.Nucleic acid amplification tests for diagnosing Clostridioides difficile infections (CDI) are improving to become faster and more accurate. This study aimed to evaluate the accuracy of rapid detection of toxigenic C. difficile using the novel high-speed polymerase chain reaction (PCR) device, PathOC RightGene. These results were compared and evaluated with real-time PCR (qPCR) and enzyme immunoassays (EIA) kit. For this study, 102 C. difficile and 3 Clostridium species isolated from CDI patients were used. These C. difficile isolates were 85 toxigenic and 17 non-toxigenic strains. The results of qPCR served as a standard, and sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the PathOC Right Gene were 99.2%, 99.4%, 100%, 98.8%, and 99.3%, respectively. Turnaround time of qPCR and EIA was 85 and 30 minutes, whereas PathOC RightGene was only 25 minutes including DNA extraction. This novel high-speed PCR device detected toxigenic C. difficile rapidly and accurately.
ArticleNumber 115247
Author Sekizawa, Ryuichi
Mitsutake, Hiroshi
Aso, Ryoko
Nakamura, Shigeki
Takemura, Hiromu
Okanda, Takashi
Matsumoto, Tetsuya
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  fullname: Nakamura, Shigeki
  organization: Department of Microbiology, Tokyo Medical University, Tokyo, Japan
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Keywords Point-of-care testing
Clostridioides difficile infection
Binary toxin
Toxin B
High-speed polymerase chain reaction
Nucleic acid amplification tests
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  article-title: Toxin B is essential for virulence of Clostridium difficile
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Snippet Nucleic acid amplification tests for diagnosing Clostridioides difficile infections (CDI) are improving to become faster and more accurate. This study aimed to...
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SubjectTerms Bacterial Toxins
Binary toxin
Clostridioides difficile
Clostridioides difficile infection
Clostridium
Clostridium Infections
Feces
High-speed polymerase chain reaction
Humans
Immunoenzyme Techniques
Molecular Diagnostic Techniques
Nucleic acid amplification tests
Point-of-Care Testing
Polymerase Chain Reaction
Sensitivity and Specificity
Toxin B
Title Rapid detection assay of toxigenic Clostridioides difficile through PathOC RightGene, a novel high-speed polymerase chain reaction device
URI https://www.clinicalkey.com/#!/content/1-s2.0-S0732889320306246
https://dx.doi.org/10.1016/j.diagmicrobio.2020.115247
https://cir.nii.ac.jp/crid/1871709542524434304
https://www.ncbi.nlm.nih.gov/pubmed/33188946
https://www.proquest.com/docview/2460761240
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