Roles of DNA helicases and Exo1 in the avoidance of mutations induced by Top1-mediated cleavage at ribonucleotides in DNA

• Published in: Cell cycle (Georgetown, Tex.) Vol. 15; no. 3; pp. 331 - 336
• Main Authors: Niu, Hengyao, Potenski, Catherine J., Epshtein, Anastasiya, Sung, Patrick, Klein, Hannah L.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.02.2016
• Subjects: Adenylyl Cyclases - genetics; Adenylyl Cyclases - metabolism; DNA - metabolism; DNA helicase; DNA Helicases - genetics; DNA Helicases - metabolism; DNA Repair; DNA Repair Enzymes - genetics; DNA Repair Enzymes - metabolism; DNA Topoisomerases, Type I - genetics; DNA Topoisomerases, Type I - metabolism; Exodeoxyribonucleases - genetics; Exodeoxyribonucleases - metabolism; Extra View; Humans; Mutagenesis; ribonucleotides; Ribonucleotides - metabolism; RNaseH2; rNMPs; Sgs1; DNA helicase; RNaseH2; Sgs1; ribonucleotides; rNMPs

The replicative DNA polymerases insert ribonucleotides into DNA at a frequency of approximately 1/6500 nucleotides replicated. The rNMP residues make the DNA backbone more susceptible to hydrolysis and can also distort the helix, impeding the transcription and replication machineries. rNMPs in DNA a...