Effect of sinomenine on cytokine expression of macrophages and synoviocytes in adjuvant arthritis rats

• Published in: Journal of ethnopharmacology Vol. 98; no. 1; pp. 37 - 43
• Main Authors: Wang, Yong, Fang, Yongfei, Huang, Wenhua, Zhou, Xin, Wang, Minghai, Zhong, Bing, Peng, Daizhi
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 08.04.2005; Elsevier
• Subjects: alkaloids; Animals; anti-inflammatory activity; Arthritis; Arthritis, Experimental - drug therapy; Arthritis, Experimental - metabolism; Arthritis, Experimental - physiopathology; Biological and medical sciences; cells; cytokines; Disease Models, Animal; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical - methods; General pharmacology; I-kappa B Proteins - drug effects; I-kappa B Proteins - genetics; I-kappa B Proteins - metabolism; Interleukin-1 - genetics; Interleukin-1 - metabolism; Macrophages; Macrophages, Peritoneal - cytology; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - metabolism; Male; Medical sciences; medicinal plants; Morphinans - chemistry; Morphinans - pharmacology; Morphinans - therapeutic use; NF-kappa B - antagonists & inhibitors; NF-kappa B - drug effects; NF-kappa B - genetics; Nuclear factor-κB; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plants, Medicinal - chemistry; Rats; Rats, Wistar; rheumatoid arthritis; RNA, Messenger; Sinomenium acutum; Synovial Membrane - cytology; Synovial Membrane - drug effects; Synovial Membrane - metabolism; Synoviocytes; Tumor Necrosis Factor-alpha - drug effects; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; Up-Regulation - drug effects; Up-Regulation - genetics; AA; IκB-α; FCA; ICAM-1; Arthritis; Tumor necrosis factor-α; IL-1β; Macrophages; Nuclear factor-κB; PVDF; Synoviocytes; LPS; ECL; RA; DMEM; NF-κB; TNF-α; EMSA; NSAIDs; RT-PCR; FCS; PMs; SN; Rat; Pharmacognosy; Rodentia; Diseases of the osteoarticular system; Cytokine; Medicinal plant; Vertebrata; Mammalia; Animal; Plant origin; Nuclear factor-KB; Tumor necrosis factor-a; Macrophage

For exploring the mechanism of the anti-inflammatory and anti-rheumatic effect of sinomenine (SN), the actions of different dosage of SN were observed in vitro on the expression of cytokines, tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β), as well as the activity of nuclear factor-κB (NF-κ...