Purification and characterization of a feruloyl esterase from the intestinal bacterium Lactobacillus acidophilus

• Published in: Applied and Environmental Microbiology Vol. 70; no. 4; pp. 2367 - 2372
• Main Authors: Wang, X, Geng, X, Egashira, Y, Sanada, H
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.04.2004
• Subjects: Amino Acid Sequence; amino acid sequences; Amino acids; Antioxidants; Bacteria; Biological and medical sciences; Carbohydrate Metabolism; carboxylic ester hydrolases; Carboxylic Ester Hydrolases - genetics; Carboxylic Ester Hydrolases - isolation & purification; Carboxylic Ester Hydrolases - metabolism; Chromatography; Corn bran; Coumaric Acids; Coumaric Acids - metabolism; Diet; Edible Grain; endo-1,4-beta-xylanase; enzyme activity; enzyme substrates; Enzymes; enzymology; Enzymology and Protein Engineering; esters; ferulic acid; feruloylated sugar esters; Fundamental and applied biological sciences. Psychology; genetics; Humans; intestinal microorganisms; Intestines; Intestines - microbiology; isolation & purification; Kinetics; Lactobacillus acidophilus; Lactobacillus acidophilus - enzymology; Lactobacillus acidophilus - genetics; metabolism; Metal concentrations; Microbiology; Molecular Sequence Data; Molecular Weight; purification; Quantitative genetics; Sequence Homology, Amino Acid; Substrate Specificity; Characterization; Lactic acid bacteria; Purification; Enzyme; Gut; Bacteria; Hydrolases; Lactobacillaceae; Esterases; Lactobacillus acidophilus

Dietary ferulic acid (FA), a significant antioxidant substance, is currently the subject of extensive research. FA in cereals exists mainly as feruloylated sugar ester. To release FA from food matrices, it is necessary to cleave ester cross-linking by feruloyl esterase (FAE) (hydroxycinnamoyl estera...