Transduction of Schistosoma japonicum schistosomules with vesicular stomatitis virus glycoprotein pseudotyped murine leukemia retrovirus and expression of reporter human telomerase reverse transcriptase in the transgenic schistosomes

• Published in: Molecular and biochemical parasitology Vol. 174; no. 2; pp. 109 - 116
• Main Authors: Yang, Shenghui, Brindley, Paul J., Zeng, Qingren, Li, Yuesheng, Zhou, Jun, Liu, Yan, Liu, Biyuan, Cai, Liting, Zeng, Tiebing, Wei, Qi, Lan, Lingmei, McManus, Donald P.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2010; Amsterdam: Elsevier
• Subjects: Animals; Animals, Genetically Modified - genetics; Animals, Genetically Modified - metabolism; Cell Line; Genes, Reporter; Glycoprotein of vesicular stomatitis virus (VSVG); Humans; Immunoblotting; Immunohistochemistry; Leukemia Virus, Murine - genetics; Leukemia Virus, Murine - metabolism; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Mice; Murine leukemia virus; NIH 3T3 Cells; Pantropic retrovirus; Polymerase Chain Reaction; Rabbits; Reverse Transcriptase Polymerase Chain Reaction; Schistosoma japonicum; Schistosoma japonicum - genetics; Schistosoma japonicum - growth & development; Schistosoma japonicum - metabolism; Schistosoma japonicum - virology; Schistosoma mansoni; Telomerase; Telomerase - genetics; Telomerase - metabolism; Transduction, Genetic; Transgene; Transgenes; Vesicular stomatitis virus; Viral Envelope Proteins - genetics; Viral Envelope Proteins - metabolism; Murine leukemia virus; Glycoprotein of vesicular stomatitis virus (VSVG); Pantropic retrovirus; Schistosoma japonicum; Transgene; Telomerase

The analyses of PCR, RT-PCR, immunohistochemistry and immunoblot indicated that Schistosoma japonicum could be effectively transduced by VSVG-pseudotyped retrovirus carrying human telomerase reverse transcriptase gene (hTERT). [Display omitted] ▶Schistosoma japonicum could be transduced by VSVG-pseu...