Evolution of mouse hepatitis virus: detection and characterization of spike deletion variants during persistent infection

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Published in	Journal of Virology Vol. 71; no. 4; pp. 2959 - 2969
Main Authors	Rowe, C L, Baker, S C, Nathan, M J, Fleming, J O
Format	Journal Article
Language	English
Published	United States American Society for Microbiology 01.04.1997
Subjects	Animals Brain - virology Cell Line Coronavirus Infections - pathology Coronavirus Infections - virology Evolution, Molecular Gene Deletion Genetic Variation Male Membrane Glycoproteins - genetics Mice Mice, Inbred C57BL Murine hepatitis virus - genetics Murine hepatitis virus - isolation & purification Murine hepatitis virus - physiology Nervous System Diseases - virology Spike Glycoprotein, Coronavirus Spinal Cord - virology Time Factors Viral Envelope Proteins - genetics Virus Latency
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Abstract	Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JVI .asm.org, visit: JVI
AbstractList	Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JVI .asm.org, visit: JVI High-frequency RNA recombination has been proposed as an important mechanism for generating viral deletion variants of murine coronavirus. Indeed, a number of variants with deletions in the spike glycoprotein have been isolated from persistently infected animals. However, the significance of generating and potentially accumulating deletion variants in the persisting viral RNA population is unclear. To study this issue, we evaluated the evolution of spike variants by examining the population of spike RNA sequences detected in the brains and spinal cords of mice inoculated with coronavirus and sacrificed at 4, 42, or 100 days postinoculation. We focused on the S1 hypervariable region since previous investigators had shown that this region is subject to recombination and deletion. RNA isolated from the brains or spinal cords of infected mice was rescued by reverse transcription-PCR, and the amplified products were cloned and used in differential colony hybridizations to identify individual isolates with deletions. We found that 11 of 20 persistently infected mice harbored spike deletion variants (SDVs), indicating that deletions are common but not required for persistent infection. To determine if a specific type of SDV accumulated during persistence, we sequenced 106 of the deletion isolates. We identified 23 distinct patterns of SDVs, including 5 double-deletion variants. Furthermore, we found that each mouse harbored distinct variants in its central nervous system (CNS), suggesting that SDVs are generated during viral replication in the CNS. Interestingly, mice with the most severe and persisting neurological disease harbored the most prevalent and diverse quasispecies of SDVs. Overall, these findings illustrate the complexity of the population of persisting viral RNAs which may contribute to chronic disease. High-frequency RNA recombination has been proposed as an important mechanism for generating viral deletion variants of murine coronavirus. Indeed, a number of variants with deletions in the spike glycoprotein have been isolated from persistently infected animals. However, the significance of generating and potentially accumulating deletion variants in the persisting viral RNA population is unclear. To study this issue, we evaluated the evolution of spike variants by examining the population of spike RNA sequences detected in the brains and spinal cords of mice inoculated with coronavirus and sacrificed at 4, 42, or 100 days postinoculation. We focused on the S1 hypervariable region since previous investigators had shown that this region is subject to recombination and deletion. RNA isolated from the brains or spinal cords of infected mice was rescued by reverse transcription-PCR, and the amplified products were cloned and used in differential colony hybridizations to identify individual isolates with deletions. We found that 11 of 20 persistently infected mice harbored spike deletion variants (SDVs), indicating that deletions are common but not required for persistent infection. To determine if a specific type of SDV accumulated during persistence, we sequenced 106 of the deletion isolates. We identified 23 distinct patterns of SDVs, including 5 double-deletion variants. Furthermore, we found that each mouse harbored distinct variants in its central nervous system (CNS), suggesting that SDVs are generated during viral replication in the CNS. Interestingly, mice with the most severe and persisting neurological disease harbored the most prevalent and diverse quasispecies of SDVs. Overall, these findings illustrate the complexity of the population of persisting viral RNAs which may contribute to chronic disease.High-frequency RNA recombination has been proposed as an important mechanism for generating viral deletion variants of murine coronavirus. Indeed, a number of variants with deletions in the spike glycoprotein have been isolated from persistently infected animals. However, the significance of generating and potentially accumulating deletion variants in the persisting viral RNA population is unclear. To study this issue, we evaluated the evolution of spike variants by examining the population of spike RNA sequences detected in the brains and spinal cords of mice inoculated with coronavirus and sacrificed at 4, 42, or 100 days postinoculation. We focused on the S1 hypervariable region since previous investigators had shown that this region is subject to recombination and deletion. RNA isolated from the brains or spinal cords of infected mice was rescued by reverse transcription-PCR, and the amplified products were cloned and used in differential colony hybridizations to identify individual isolates with deletions. We found that 11 of 20 persistently infected mice harbored spike deletion variants (SDVs), indicating that deletions are common but not required for persistent infection. To determine if a specific type of SDV accumulated during persistence, we sequenced 106 of the deletion isolates. We identified 23 distinct patterns of SDVs, including 5 double-deletion variants. Furthermore, we found that each mouse harbored distinct variants in its central nervous system (CNS), suggesting that SDVs are generated during viral replication in the CNS. Interestingly, mice with the most severe and persisting neurological disease harbored the most prevalent and diverse quasispecies of SDVs. Overall, these findings illustrate the complexity of the population of persisting viral RNAs which may contribute to chronic disease.
Author	J O Fleming C L Rowe S C Baker M J Nathan
AuthorAffiliation	Department of Microbiology and Immunology, Loyola University of Chicago, Stritch School of Medicine, Maywood, Illinois 60153, USA
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References	6091331 - Virology. 1984 Sep;137(2):297-304 2848172 - Microb Pathog. 1987 Jul;3(1):9-20 7685402 - J Virol. 1993 Jul;67(7):3854-60 1656597 - Virology. 1991 Nov;185(1):441-5 8430521 - Adv Virus Res. 1993;42:249-324 3009966 - Lab Anim Sci. 1986 Apr;36(2):130-5 1316938 - J Gen Virol. 1992 May;73 ( Pt 5):1065-72 2836513 - J Immunol Methods. 1988 May 25;110(1):11-8 2447229 - J Gen Virol. 1988 Jan;69 ( Pt 1):87-98 2833625 - J Virol. 1988 May;62(5):1810-3 271968 - Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 1379178 - EMBO J. 1992 Aug;11(8):3135-45 6281979 - Virology. 1982 Jun;119(2):358-71 6256683 - Neurology. 1981 Jan;31(1):38-44 2158184 - Virology. 1990 Apr;175(2):548-55 3701929 - J Virol. 1986 Jun;58(3):869-75 2448875 - Science. 1988 Jan 29;239(4839):487-91 1850936 - Virology. 1991 Jun;182(2):883-8 8264403 - Methods Enzymol. 1993;224:428-38 8627236 - J Gen Virol. 1996 Feb;77 ( Pt 2 ):315-25 1310555 - Virology. 1992 Mar;187(1):178-88 1688627 - J Virol. 1990 Feb;64(2):731-41 7827789 - Infect Agents Dis. 1994 Aug;3(4):201-14 7533851 - J Virol. 1995 Apr;69(4):2187-93 6312277 - Microbiol Immunol. 1983;27(5):445-54 7515165 - Nature. 1994 Jun 2;369(6479):403-7 8276276 - Gene. 1993 Dec 15;135(1-2):37-47 3005623 - J Virol. 1986 Mar;57(3):729-37 8411355 - J Virol. 1993 Nov;67(11):6522-34 11831732 - Virology. 1995 Apr 1;208(1):67-74 1310195 - Virology. 1992 Feb;186(2):742-9 8830547 - Adv Exp Med Biol. 1995;380:591-5 1579113 - Microbiol Rev. 1992 Mar;56(1):61-79 2999443 - J Virol. 1985 Dec;56(3):904-11 1318185 - Curr Top Microbiol Immunol. 1992;176:33-47 3021340 - Cell. 1986 Nov 7;47(3):433-43 8808680 - Immunity. 1996 Sep;5(3):253-62 6301992 - Infect Immun. 1983 Mar;39(3):1316-24 7520468 - J Exp Med. 1994 Sep 1;180(3):1129-34 3016306 - J Virol. 1986 Aug;59(2):463-71 7791879 - Nature. 1995 Jun 15;375(6532):606-11 3027248 - J Gen Virol. 1987 Jan;68 ( Pt 1):47-56 1313927 - J Virol. 1992 May;66(5):3225-9 7494335 - J Virol. 1995 Dec;69(12):8127-31 8102242 - Reg Immunol. 1993 Jan-Feb;5(1):37-43 2825419 - Virology. 1987 Dec;161(2):479-87 2556846 - Virology. 1989 Dec;173(2):664-73 8196768 - Nature. 1994 Jun 2;369(6479):407-10 7778268 - Virology. 1995 Jun 1;209(2):337-46 2538027 - Virology. 1989 Mar;169(1):127-36
References_xml	– reference: 6091331 - Virology. 1984 Sep;137(2):297-304 – reference: 2538027 - Virology. 1989 Mar;169(1):127-36 – reference: 1318185 - Curr Top Microbiol Immunol. 1992;176:33-47 – reference: 1850936 - Virology. 1991 Jun;182(2):883-8 – reference: 7685402 - J Virol. 1993 Jul;67(7):3854-60 – reference: 2158184 - Virology. 1990 Apr;175(2):548-55 – reference: 3701929 - J Virol. 1986 Jun;58(3):869-75 – reference: 2825419 - Virology. 1987 Dec;161(2):479-87 – reference: 2447229 - J Gen Virol. 1988 Jan;69 ( Pt 1):87-98 – reference: 8627236 - J Gen Virol. 1996 Feb;77 ( Pt 2 ):315-25 – reference: 8808680 - Immunity. 1996 Sep;5(3):253-62 – reference: 7827789 - Infect Agents Dis. 1994 Aug;3(4):201-14 – reference: 2848172 - Microb Pathog. 1987 Jul;3(1):9-20 – reference: 1313927 - J Virol. 1992 May;66(5):3225-9 – reference: 1688627 - J Virol. 1990 Feb;64(2):731-41 – reference: 7533851 - J Virol. 1995 Apr;69(4):2187-93 – reference: 3027248 - J Gen Virol. 1987 Jan;68 ( Pt 1):47-56 – reference: 2833625 - J Virol. 1988 May;62(5):1810-3 – reference: 7791879 - Nature. 1995 Jun 15;375(6532):606-11 – reference: 2448875 - Science. 1988 Jan 29;239(4839):487-91 – reference: 7515165 - Nature. 1994 Jun 2;369(6479):403-7 – reference: 11831732 - Virology. 1995 Apr 1;208(1):67-74 – reference: 1579113 - Microbiol Rev. 1992 Mar;56(1):61-79 – reference: 2836513 - J Immunol Methods. 1988 May 25;110(1):11-8 – reference: 6312277 - Microbiol Immunol. 1983;27(5):445-54 – reference: 1379178 - EMBO J. 1992 Aug;11(8):3135-45 – reference: 6301992 - Infect Immun. 1983 Mar;39(3):1316-24 – reference: 8102242 - Reg Immunol. 1993 Jan-Feb;5(1):37-43 – reference: 8411355 - J Virol. 1993 Nov;67(11):6522-34 – reference: 8264403 - Methods Enzymol. 1993;224:428-38 – reference: 3009966 - Lab Anim Sci. 1986 Apr;36(2):130-5 – reference: 1656597 - Virology. 1991 Nov;185(1):441-5 – reference: 8830547 - Adv Exp Med Biol. 1995;380:591-5 – reference: 3005623 - J Virol. 1986 Mar;57(3):729-37 – reference: 8276276 - Gene. 1993 Dec 15;135(1-2):37-47 – reference: 8196768 - Nature. 1994 Jun 2;369(6479):407-10 – reference: 3016306 - J Virol. 1986 Aug;59(2):463-71 – reference: 8430521 - Adv Virus Res. 1993;42:249-324 – reference: 6281979 - Virology. 1982 Jun;119(2):358-71 – reference: 2999443 - J Virol. 1985 Dec;56(3):904-11 – reference: 2556846 - Virology. 1989 Dec;173(2):664-73 – reference: 7778268 - Virology. 1995 Jun 1;209(2):337-46 – reference: 1310555 - Virology. 1992 Mar;187(1):178-88 – reference: 271968 - Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 – reference: 6256683 - Neurology. 1981 Jan;31(1):38-44 – reference: 7494335 - J Virol. 1995 Dec;69(12):8127-31 – reference: 3021340 - Cell. 1986 Nov 7;47(3):433-43 – reference: 1310195 - Virology. 1992 Feb;186(2):742-9 – reference: 7520468 - J Exp Med. 1994 Sep 1;180(3):1129-34 – reference: 1316938 - J Gen Virol. 1992 May;73 ( Pt 5):1065-72
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Snippet	Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley... High-frequency RNA recombination has been proposed as an important mechanism for generating viral deletion variants of murine coronavirus. Indeed, a number of...
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SubjectTerms	Animals Brain - virology Cell Line Coronavirus Infections - pathology Coronavirus Infections - virology Evolution, Molecular Gene Deletion Genetic Variation Male Membrane Glycoproteins - genetics Mice Mice, Inbred C57BL Murine hepatitis virus - genetics Murine hepatitis virus - isolation & purification Murine hepatitis virus - physiology Nervous System Diseases - virology Spike Glycoprotein, Coronavirus Spinal Cord - virology Time Factors Viral Envelope Proteins - genetics Virus Latency
Title	Evolution of mouse hepatitis virus: detection and characterization of spike deletion variants during persistent infection
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