emPAI‐assisted strategy enhances screening and assessment of Mycobacterium tuberculosis infection serological markers
Summary Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance ind...
Saved in:
| Published in | Microbial biotechnology Vol. 14; no. 4; pp. 1827 - 1838 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
John Wiley & Sons, Inc
01.07.2021
John Wiley and Sons Inc Wiley |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Summary
Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value‐assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC‐MS/MS to analyse MTB culture filtrate proteins (MTB‐CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB‐CFPs – including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value > 1.0 and recombinantly expressed these proteins in Escherichia coli. At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R‐squared value ranged from 0.64 to 0.79. The only exception was ESAT‐6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI‐assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future.
The areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934, and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value, and antibody affinity in mice immunized with recombinant MTB‐protein showed that emPAI values were positively correlated with them, and R squared value ranged from 0.64 to 0.79. This study provides a rationale for the serological marker screening of emPAI‐assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. |
|---|---|
| AbstractList | Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value-assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC-MS/MS to analyse MTB culture filtrate proteins (MTB-CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB-CFPs - including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value > 1.0 and recombinantly expressed these proteins in Escherichia coli. At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R-squared value ranged from 0.64 to 0.79. The only exception was ESAT-6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI-assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future.Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value-assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC-MS/MS to analyse MTB culture filtrate proteins (MTB-CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB-CFPs - including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value > 1.0 and recombinantly expressed these proteins in Escherichia coli. At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R-squared value ranged from 0.64 to 0.79. The only exception was ESAT-6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI-assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value‐assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC‐MS/MS to analyse MTB culture filtrate proteins (MTB‐CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB‐CFPs – including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value > 1.0 and recombinantly expressed these proteins in Escherichia coli . At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R‐squared value ranged from 0.64 to 0.79. The only exception was ESAT‐6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI‐assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value-assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC-MS/MS to analyse MTB culture filtrate proteins (MTB-CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB-CFPs - including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value > 1.0 and recombinantly expressed these proteins in Escherichia coli. At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R-squared value ranged from 0.64 to 0.79. The only exception was ESAT-6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI-assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value‐assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC‐MS/MS to analyse MTB culture filtrate proteins (MTB‐CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB‐CFPs – including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value > 1.0 and recombinantly expressed these proteins in Escherichia coli. At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R‐squared value ranged from 0.64 to 0.79. The only exception was ESAT‐6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI‐assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value‐assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC‐MS/MS to analyse MTB culture filtrate proteins (MTB‐CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB‐CFPs – including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value > 1.0 and recombinantly expressed these proteins in Escherichia coli . At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R‐squared value ranged from 0.64 to 0.79. The only exception was ESAT‐6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI‐assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. The areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934, and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value, and antibody affinity in mice immunized with recombinant MTB‐protein showed that emPAI values were positively correlated with them, and R squared value ranged from 0.64 to 0.79. This study provides a rationale for the serological marker screening of emPAI‐assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. Summary Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value‐assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC‐MS/MS to analyse MTB culture filtrate proteins (MTB‐CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB‐CFPs – including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value > 1.0 and recombinantly expressed these proteins in Escherichia coli. At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R‐squared value ranged from 0.64 to 0.79. The only exception was ESAT‐6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI‐assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. Summary Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great significance for the prevention and control of tuberculosis. In this study, we established an exponentially modified protein abundance index (emPAI) value‐assisted strategy to investigate and improve the screening efficiency of serological biomarkers of tuberculosis. First, we used LC‐MS/MS to analyse MTB culture filtrate proteins (MTB‐CFPs), and 632 MTB proteins were identified. Then, the characteristic values of MTB‐CFPs – including emPAI value, molecular weight (Mw), isoelectric point (pI), grand average of hydropathy (GRAVY), transmembrane domain (TMD) and functional groups were calculated. Next, we successfully prepared 10 MTB proteins with emPAI value > 1.0 and recombinantly expressed these proteins in Escherichia coli. At the same time, 3 MTB proteins with emPAI between 0.1 and 0.5 were randomly selected as the control groups, and the immunogenicity of the recombinant MTB proteins was detected using ELISA. The sensitivity and receiver operating characteristic (ROC) curves were calculated for each recombinant MTB protein. The results showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant MTB protein showed that emPAI values were positively correlated with them, and R‐squared value ranged from 0.64 to 0.79. The only exception was ESAT‐6 (encoded by the Rv3875 gene), which AUC value was relatively low owing to its strong immunosuppressive properties. This study provides a rationale for the serological marker screening of emPAI‐assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. The areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934, and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value, and antibody affinity in mice immunized with recombinant MTB‐protein showed that emPAI values were positively correlated with them, and R squared value ranged from 0.64 to 0.79. This study provides a rationale for the serological marker screening of emPAI‐assisted tuberculosis clinical test. The results also provide new technical support for the screening of candidate serological markers of infectious diseases in the future. |
| Author | Wang, Wei Ma, Guorong Zhou, Lin Ouyang, Junlin Li, Rongxiu Zhang, Shulin Wang, Pei Yang, Yanhui Ma, Jinhua |
| AuthorAffiliation | 4 State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China 2 Ningxia Key Laboratory of Prevention and Control of Common Infectious Diseases Ningxia Medical University Yinchuan 750004 China 6 Tuberculosis Research Center School of Medicine Shanghai Public Health Clinical Center Shanghai 201508 China 3 College of Biological Science and Engineering Northern University for Nationalities Yinchuan 750021 China 1 School of Basic Medical Sciences Ningxia Medical University Yinchuan 750004 China 5 Department of Immunology and Microbiology School of Medicine Shanghai Jiao Tong University Shanghai 200025 China |
| AuthorAffiliation_xml | – name: 3 College of Biological Science and Engineering Northern University for Nationalities Yinchuan 750021 China – name: 4 State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China – name: 2 Ningxia Key Laboratory of Prevention and Control of Common Infectious Diseases Ningxia Medical University Yinchuan 750004 China – name: 1 School of Basic Medical Sciences Ningxia Medical University Yinchuan 750004 China – name: 6 Tuberculosis Research Center School of Medicine Shanghai Public Health Clinical Center Shanghai 201508 China – name: 5 Department of Immunology and Microbiology School of Medicine Shanghai Jiao Tong University Shanghai 200025 China |
| Author_xml | – sequence: 1 givenname: Guorong orcidid: 0000-0003-3308-8021 surname: Ma fullname: Ma, Guorong organization: Ningxia Medical University – sequence: 2 givenname: Pei surname: Wang fullname: Wang, Pei organization: Ningxia Medical University – sequence: 3 givenname: Yanhui surname: Yang fullname: Yang, Yanhui organization: Ningxia Medical University – sequence: 4 givenname: Wei surname: Wang fullname: Wang, Wei organization: Northern University for Nationalities – sequence: 5 givenname: Jinhua surname: Ma fullname: Ma, Jinhua organization: Ningxia Medical University – sequence: 6 givenname: Lin surname: Zhou fullname: Zhou, Lin organization: Ningxia Medical University – sequence: 7 givenname: Junlin surname: Ouyang fullname: Ouyang, Junlin organization: Ningxia Medical University – sequence: 8 givenname: Rongxiu surname: Li fullname: Li, Rongxiu email: rxli@sjtu.edu.cn organization: Shanghai Jiao Tong University – sequence: 9 givenname: Shulin surname: Zhang fullname: Zhang, Shulin email: shulinzhang@sjtu.edu.cn organization: Shanghai Public Health Clinical Center |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34173722$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFks1u1DAUhSNURH9gzQ5FYsNmWv8mkw1SWxUYqRUsytq6sa9TD4ld7ASYXR-BR-BZeBSeBE-nrdpKCG9sXX_n-Oj67hZbPngsipeU7NO8Dmgt6axuqNynfM6aJ8XOXWXr3nm72E1pSUhFiGTPim0uaM1rxnaKHzh8Olz8ufoJKbk0oinTGGHEblWivwCvMZVJR0TvfFeCN2UGMaUB_VgGW56tdGhBjxjdNPz-NU4tRj31IZuVzlvUowu-TBhDHzqnoS8HiF8wpufFUwt9whc3-17x-d3J-fGH2enH94vjw9OZllw0MwBCRM2NhJYbWxlrTU0BJauEoYyJFq1ECaI1gmrCiJHcMqzqRlhDRIV8r1hsfE2ApbqMLr-_UgGcui6E2CmIo9M9Km2taFA2GjgRhLQtMQyBz4mpGkYZzV5vN16XUzug0bkHEfoHpg9vvLtQXfim5pzynDgbvLkxiOHrhGlUg0sa-x48hikpJoWsyLxmTUZfP0KXYYo-typTUtKmqed1pl7dT3QX5faDMyA3gI4hpYhWaTfC-lNyQNcrStR6kNR6VNR6VNT1IGXdwSPdrfW_FdVG8d31uPofrs6OztlG-BeiGt22 |
| CitedBy_id | crossref_primary_10_1371_journal_pone_0275992 crossref_primary_10_1186_s12934_023_02271_3 |
| Cites_doi | 10.1002/pmic.201600177 10.1128/CVI.00198-10 10.1128/JCM.43.1.57-65.2005 10.1183/09031936.00114810 10.1371/journal.ppat.1004446 10.1002/pmic.200600853 10.1111/1751-7915.13291 10.1074/mcp.M112.018846 10.1074/mcp.M500084-MCP200 10.1016/S1473-3099(13)70034-3 10.1111/j.1574-695X.2011.00838.x 10.1038/nm.2285 10.1016/j.bbapap.2020.140470 10.1074/mcp.M113.032623 10.1073/pnas.1009080107 10.1093/bioinformatics/btp700 10.1186/s12879-017-2910-y 10.1111/1751-7915.12697 10.1016/j.tube.2011.11.005 10.1016/j.celrep.2014.11.023 10.1016/j.jchromb.2017.07.052 10.1590/S1806-37132009000500012 10.1111/j.1365-3083.2007.01978.x 10.1016/S0378-1097(04)00008-4 10.1074/mcp.M500061-MCP200 |
| ContentType | Journal Article |
| Copyright | 2021 The Authors. published by John Wiley & Sons Ltd and Society for Applied Microbiology. 2021 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: 2021 The Authors. published by John Wiley & Sons Ltd and Society for Applied Microbiology. – notice: 2021 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. – notice: 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | 24P AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7QO 7T7 7X7 7XB 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ HCIFZ K9. L6V LK8 M0S M7P M7S P64 PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI PRINS PTHSS RC3 7X8 5PM DOA |
| DOI | 10.1111/1751-7915.13829 |
| DatabaseName | Wiley Online Library Open Access CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Biotechnology Research Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central (New) Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) ProQuest Engineering Collection ProQuest Biological Science Collection ProQuest Health & Medical Collection Biological Science Database Engineering Database Biotechnology and BioEngineering Abstracts ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest Central Student Technology Collection Technology Research Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Sustainability Genetics Abstracts ProQuest Engineering Collection Health Research Premium Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection Industrial and Applied Microbiology Abstracts (Microbiology A) ProQuest Central (New) Engineering Collection Engineering Database ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Materials Science & Engineering Collection Engineering Research Database ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic CrossRef MEDLINE Publicly Available Content Database |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: 24P name: Wiley Online Library Open Access url: https://authorservices.wiley.com/open-science/open-access/browse-journals.html sourceTypes: Publisher – sequence: 3 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 4 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 5 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering |
| DocumentTitleAlternate | emPAI strategy enhance screening of TB markers |
| EISSN | 1751-7915 |
| EndPage | 1838 |
| ExternalDocumentID | oai_doaj_org_article_cff49e59ca30400bb0d2ea380d692121 PMC8313264 34173722 10_1111_1751_7915_13829 MBT213829 |
| Genre | article Research Support, Non-U.S. Gov't Journal Article |
| GrantInformation_xml | – fundername: Ningxia hui autonomous region education department outstanding young teachers training fund project funderid: NGY2018‐79 – fundername: Superior discipline group (Orthopedics) construction research project in 2019 of Ningxia medical university, The third batch of special talents launched in 2017 of Ningxia medical university funderid: XT2017028 – fundername: Ningxia hui autonomous region key research and development funderid: 2018BEB04033 – fundername: The Ningxia hui autonomous region key R&D funderid: 2019BEG03065 – fundername: Undergraduate innovation and entrepreneurship project of Ningxia Hui Autonomous Region funderid: S201910752013 – fundername: The National Science & Technology Major Projects of China funderid: 2017ZX10201301‐003‐004 – fundername: Ningxia hui autonomous region key research and development grantid: 2018BEB04033 – fundername: Superior discipline group (Orthopedics) construction research project in 2019 of Ningxia medical university, The third batch of special talents launched in 2017 of Ningxia medical university grantid: XT2017028 – fundername: Undergraduate innovation and entrepreneurship project of Ningxia Hui Autonomous Region grantid: S201910752013 – fundername: Ningxia hui autonomous region education department outstanding young teachers training fund project grantid: NGY2018-79 – fundername: The National Science & Technology Major Projects of China grantid: 2017ZX10201301-003-004 – fundername: The Ningxia hui autonomous region key R&D grantid: 2019BEG03065 – fundername: The National Science & Technology Major Projects of China grantid: 2017ZX10201301‐003‐004 – fundername: Ningxia hui autonomous region education department outstanding young teachers training fund project grantid: NGY2018‐79 |
| GroupedDBID | --- 0R~ 123 1OC 24P 29M 31~ 4.4 53G 5DZ 5VS 7X7 8-1 8FE 8FG 8FH 8FI 8FJ A8Z AAMMB AANHP AAZKR ABDBF ABJCF ABUWG ACBWZ ACCMX ACGFO ACIWK ACPRK ACRPL ACUHS ACXQS ACYXJ ADBBV ADKYN ADNMO ADPDF ADRAZ ADZMN AEFGJ AEGXH AENEX AEUYN AFKRA AFRAH AGQPQ AGXDD AHMBA AIAGR AIDQK AIDYY ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AOIJS ASPBG AVUZU AVWKF AZFZN BAWUL BBNVY BCNDV BDRZF BENPR BGLVJ BHPHI BPHCQ BVXVI CAG CCPQU COF CS3 D-9 DIK EBD EBS EJD EMOBN ESX F5P FEDTE FYUFA GODZA GROUPED_DOAJ GX1 HCIFZ HMCUK HVGLF HYE IAO IHR ITC KQ8 L6V LH4 LK8 LW6 M48 M7P M7S ML0 MM. O9- OIG OK1 OVD OVEED P2P PHGZM PHGZT PIMPY PQGLB PQQKQ PROAC PTHSS RNS RPM SV3 TEORI TUS UKHRP WIN AAHHS AAYXX ACCFJ AEEZP AEQDE AIWBW AJBDE ALIPV CITATION CGR CUY CVF ECM EIF NPM 3V. 7QO 7T7 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ K9. P64 PKEHL PQEST PQUKI PRINS RC3 7X8 5PM PUEGO |
| ID | FETCH-LOGICAL-c5349-aa00473d5ab3df6dffd71ae5264d1224bef5e5a4bd41c020d53f2e6794fd046e3 |
| IEDL.DBID | M48 |
| ISSN | 1751-7915 |
| IngestDate | Wed Aug 27 01:14:52 EDT 2025 Thu Aug 21 14:13:38 EDT 2025 Fri Jul 11 11:20:27 EDT 2025 Wed Aug 13 04:18:50 EDT 2025 Wed Feb 19 02:06:28 EST 2025 Tue Jul 01 03:38:02 EDT 2025 Thu Apr 24 23:08:21 EDT 2025 Wed Aug 20 07:25:44 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Language | English |
| License | Attribution 2021 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c5349-aa00473d5ab3df6dffd71ae5264d1224bef5e5a4bd41c020d53f2e6794fd046e3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| ORCID | 0000-0003-3308-8021 |
| OpenAccessLink | https://www.proquest.com/docview/2555199787?pq-origsite=%requestingapplication% |
| PMID | 34173722 |
| PQID | 2555199787 |
| PQPubID | 1016378 |
| PageCount | 12 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_cff49e59ca30400bb0d2ea380d692121 pubmedcentral_primary_oai_pubmedcentral_nih_gov_8313264 proquest_miscellaneous_2545608729 proquest_journals_2555199787 pubmed_primary_34173722 crossref_citationtrail_10_1111_1751_7915_13829 crossref_primary_10_1111_1751_7915_13829 wiley_primary_10_1111_1751_7915_13829_MBT213829 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | July 2021 |
| PublicationDateYYYYMMDD | 2021-07-01 |
| PublicationDate_xml | – month: 07 year: 2021 text: July 2021 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: Bedford – name: Hoboken |
| PublicationTitle | Microbial biotechnology |
| PublicationTitleAlternate | Microb Biotechnol |
| PublicationYear | 2021 |
| Publisher | John Wiley & Sons, Inc John Wiley and Sons Inc Wiley |
| Publisher_xml | – name: John Wiley & Sons, Inc – name: John Wiley and Sons Inc – name: Wiley |
| References | 2010; 107 2011 2010; 17 2019; 14 2017a; 1061–1062 2005; 43 2011; 37 2011; 17 2004; 231 2012; 92 2009; 35 2010; 26 2001 2013; 13 2017; 17 2013; 12 2017b; 17 2017; 10 2020; 1868 2005; 4 2011; 63 2016; 41 2014; 13 2007; 7 2007; 4 2014; 9 2018; 11 2007; 66 2014; 10 e_1_2_8_28_1 e_1_2_8_29_1 e_1_2_8_24_1 e_1_2_8_25_1 e_1_2_8_26_1 e_1_2_8_27_1 Atefeh Seghatoleslam M.H. (e_1_2_8_8_1) 2016; 41 e_1_2_8_2_1 e_1_2_8_5_1 e_1_2_8_4_1 e_1_2_8_6_1 e_1_2_8_20_1 e_1_2_8_22_1 Annabel Baddeley A.D. (e_1_2_8_7_1) 2019; 14 e_1_2_8_23_1 e_1_2_8_17_1 e_1_2_8_18_1 e_1_2_8_19_1 e_1_2_8_13_1 e_1_2_8_14_1 e_1_2_8_15_1 e_1_2_8_16_1 Awaidy S. A. (e_1_2_8_9_1) 2011 e_1_2_8_10_1 e_1_2_8_31_1 e_1_2_8_11_1 Abbas A.K. (e_1_2_8_3_1) 2001 e_1_2_8_12_1 Philip C.H. (e_1_2_8_21_1) 2007; 4 e_1_2_8_30_1 |
| References_xml | – volume: 26 start-page: 576 year: 2010 end-page: 577 article-title: emPAI Calc—for the estimation of protein abundance from large‐scale identification data by liquid chromatography‐tandem mass spectrometry publication-title: Bioinformatics – volume: 66 start-page: 176 year: 2007 end-page: 191 article-title: Progress in serodiagnosis of infection publication-title: Scand J Immunol – volume: 10 start-page: 604 year: 2017 end-page: 611 article-title: Evaluation of Rv0220, Rv2958c, Rv2994 and Rv3347c of for serodiagnosis of tuberculosis publication-title: Microb Biotechnol – volume: 43 start-page: 57 year: 2005 end-page: 65 article-title: Assessing the serodiagnostic potential of 35 proteins and identification of four novel serological antigens publication-title: J Clin Microbiol – volume: 12 start-page: 1180 year: 2013 end-page: 1191 article-title: Proteomic profiling of identifies nutrient‐starvation‐responsive toxin‐antitoxin systems publication-title: Mol Cell Proteomics – volume: 4 start-page: 1265 year: 2005 end-page: 1272 article-title: Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein publication-title: Mol Cell Proteomics – volume: 7 start-page: 1702 year: 2007 end-page: 1718 article-title: Comprehensive analysis of exported proteins H37Rv publication-title: Proteomics – volume: 17 start-page: 189 year: 2011 end-page: 194 article-title: A multistage tuberculosis vaccine that confers efficient protection before and after exposure publication-title: Nat Med – volume: 17 start-page: 1 year: 2017 end-page: 8 article-title: ORFeome‐based identification of biomarkers for serodiagnosis of latent infection publication-title: BMC Infect Dis – volume: 1061–1062 start-page: 452 year: 2017a end-page: 458 article-title: Identification of antigens in the sera of tuberculosis patients using biomimetic affinity chromatography in conjunction with ESI‐CID‐MS/MS publication-title: J Chromatogr B Analyt Technol Biomed Life Sci – volume: 231 start-page: 283 year: 2004 end-page: 289 article-title: Mycobacterium smegmatis displays the ‐related neutral red character when expressing theRv0577gene publication-title: FEMS Microbiol Lett – volume: 17 year: 2017b article-title: Improving basic and membrane protein MS detection of the culture filtrate proteins from H37Rv by biomimetic affinity prefractionation publication-title: Proteomics – volume: 14 start-page: 1 year: 2019 end-page: 297 article-title: global Tuberculosis report 2019 publication-title: World health orgnization – volume: 35 start-page: 460 year: 2009 end-page: 463 article-title: Guided sputum sample collection and culture contamination rates in the diagnosis of pulmonary TB publication-title: J Bras Pneumol – volume: 13 start-page: 362 year: 2013 end-page: 372 article-title: Tuberculosis biomarkers discovery: developments, needs, and challenges publication-title: Lancet Infect Dis – volume: 10 year: 2014 article-title: The ESAT‐6 protein of interacts with beta‐2‐microglobulin (beta2M) affecting antigen presentation function of macrophage publication-title: PLoS Pathog – start-page: 1 year: 2001 end-page: 322 – volume: 13 start-page: 897 year: 2014 end-page: 906 article-title: Exploration of novel cellular and serological antigen biomarkers in the ORFeome of publication-title: Mol Cell Proteomics – volume: 4 start-page: 1061 year: 2007 end-page: 1069 article-title: Longitudinal assessment of an ELISPOT test for infection publication-title: PLoS Med – volume: 17 start-page: 1539 year: 2010 end-page: 1547 article-title: Identification of Mycobacterium tuberculosis antigens of high serodiagnostic value publication-title: Clin Vaccine Immunol – volume: 1868 start-page: 140470 year: 2020 article-title: ESAT‐6 regulates autophagous response through SOD‐2 and as a result induces intracellular survival of BCG publication-title: Biochim Biophys Acta Proteins Proteom – volume: 92 start-page: 121 year: 2012 end-page: 132 article-title: The ins and outs of protein export publication-title: Tuberculosis – volume: 107 start-page: 14703 year: 2010 end-page: 14708 article-title: Dynamic antibody responses to the proteome publication-title: Proc Natl Acad Sci USA – volume: 63 start-page: 165 year: 2011 end-page: 173 article-title: Interferon gamma release assay in diagnosis of pediatric tuberculosis: a meta‐analysis publication-title: FEMS Immunol Med Microbiol – volume: 37 start-page: 100 year: 2011 end-page: 111 article-title: Interferon‐γ release assays for the diagnosis of active tuberculosis: a systematic review and meta‐analysis publication-title: Eur Respir J – volume: 11 start-page: 893 year: 2018 end-page: 904 article-title: Identification of new diagnostic biomarkers for and the potential application in the serodiagnosis of human tuberculosis publication-title: Microb Biotechnol – volume: 9 start-page: 2317 year: 2014 end-page: 2329 article-title: Mycobacterium tuberculosis proteome microarray for global studies of protein function and immunogenicity publication-title: Cell Rep – volume: 41 start-page: 296 year: 2016 end-page: 304 article-title: Macrophage immune response suppression by recombinant antigens, the ESAT‐6, CFP‐10, and ESAT‐6_CFP‐10 fusion proteins publication-title: Iran J Med Sci – start-page: 1 year: 2011 end-page: 26 – volume: 4 start-page: 1487 year: 2005 end-page: 1502 article-title: Comparison of label‐free methods for quantifying human proteins by shotgun proteomics publication-title: Mol Cell Proteomics – ident: e_1_2_8_17_1 doi: 10.1002/pmic.201600177 – volume: 14 start-page: 1 year: 2019 ident: e_1_2_8_7_1 article-title: global Tuberculosis report 2019 publication-title: World health orgnization – ident: e_1_2_8_11_1 doi: 10.1128/CVI.00198-10 – ident: e_1_2_8_28_1 doi: 10.1128/JCM.43.1.57-65.2005 – ident: e_1_2_8_24_1 doi: 10.1183/09031936.00114810 – ident: e_1_2_8_26_1 doi: 10.1371/journal.ppat.1004446 – ident: e_1_2_8_19_1 doi: 10.1002/pmic.200600853 – ident: e_1_2_8_22_1 doi: 10.1111/1751-7915.13291 – ident: e_1_2_8_5_1 doi: 10.1074/mcp.M112.018846 – ident: e_1_2_8_20_1 doi: 10.1074/mcp.M500084-MCP200 – ident: e_1_2_8_23_1 doi: 10.1016/S1473-3099(13)70034-3 – ident: e_1_2_8_27_1 doi: 10.1111/j.1574-695X.2011.00838.x – volume: 4 start-page: 1061 year: 2007 ident: e_1_2_8_21_1 article-title: Longitudinal assessment of an ELISPOT test for Mycobacterium tuberculosis infection publication-title: PLoS Med – ident: e_1_2_8_2_1 doi: 10.1038/nm.2285 – ident: e_1_2_8_29_1 doi: 10.1016/j.bbapap.2020.140470 – ident: e_1_2_8_15_1 doi: 10.1074/mcp.M113.032623 – ident: e_1_2_8_13_1 doi: 10.1073/pnas.1009080107 – volume: 41 start-page: 296 year: 2016 ident: e_1_2_8_8_1 article-title: Macrophage immune response suppression by recombinant Mycobacterium tuberculosis antigens, the ESAT‐6, CFP‐10, and ESAT‐6_CFP‐10 fusion proteins publication-title: Iran J Med Sci – ident: e_1_2_8_25_1 doi: 10.1093/bioinformatics/btp700 – ident: e_1_2_8_31_1 doi: 10.1186/s12879-017-2910-y – ident: e_1_2_8_30_1 doi: 10.1111/1751-7915.12697 – start-page: 1 volume-title: Commercial Serodiagnostic Tests for Diagnosis of Tuberculosis:Policy Statement year: 2011 ident: e_1_2_8_9_1 – ident: e_1_2_8_14_1 doi: 10.1016/j.tube.2011.11.005 – ident: e_1_2_8_10_1 doi: 10.1016/j.celrep.2014.11.023 – start-page: 1 volume-title: Basic Immunology: Functions and Disorders of the Immune System year: 2001 ident: e_1_2_8_3_1 – ident: e_1_2_8_16_1 doi: 10.1016/j.jchromb.2017.07.052 – ident: e_1_2_8_18_1 doi: 10.1590/S1806-37132009000500012 – ident: e_1_2_8_4_1 doi: 10.1111/j.1365-3083.2007.01978.x – ident: e_1_2_8_6_1 doi: 10.1016/S0378-1097(04)00008-4 – ident: e_1_2_8_12_1 doi: 10.1074/mcp.M500061-MCP200 |
| SSID | ssj0060052 |
| Score | 2.2727573 |
| Snippet | Summary
Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of... Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great... Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of great... Summary Discovering new serological markers of Mycobacterium tuberculosis (MTB) infection and establishing a rapid and efficient detection technology is of... |
| SourceID | doaj pubmedcentral proquest pubmed crossref wiley |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 1827 |
| SubjectTerms | Animals Antibodies Antigens Antigens, Bacterial Bacterial Proteins - genetics Biomarkers Chromatography, Liquid E coli Enzyme-linked immunosorbent assay Filtrate Functional groups Gravy Immunization Immunogenicity Infections Infectious diseases Mathematical analysis Metabolism Mice Molecular weight Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Peptides Proteins Screening Serology Tandem Mass Spectrometry Technical services Tuberculosis Tuberculosis - diagnosis Virulence |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3LatwwFBUhq2ZR-kgbt2lRIYtunIwfkq1lUhrSwoQsEshO6HFFAhlPmRlDs-sn9BP6Lf2UfknvlR_M0JZsujAYSzZC517pXOvqiLEDl1tEvZRp5RQGKMEWqbICUjC0i9PjFffCTM_l2VX5-Vpcrx31RTlhnTxw13FHLoRSgVDOFGRv1k58DqaoJ14qHHZj4INz3hBMdWOwpL-dvZAP5e3gHJmRMKM4JMk9tTEHRan-v_HLP9Mk1-lrnH9On7DHPXHkx12Dn7ItaJ6xnTU5wefsK8wujj_9-vYdCTGh5_my056959DcELxLjqMERq5YnZvGczPqcvJ54NN7h94d1Zvb2c8fq9bCwrV3c_wYH5K2Go42OwyYfEbJPYvlLrs6_Xj54SztT1ZInShKlRpDKpGFF8YWPkgfgq8yAwLZkaelNgtBgDCl9WXmkFB6UYQcJPpu8BhQQ_GCbTfzBvYYD5LUbTMnAYEyYmKdC6qulQErMoyWEnY49LV2vew4nX5xp4fwg8DRBI6O4CTs_fjCl05x499VTwi8sRpJZccHaEC6NyD9kAElbH-AXvf-u9QYaAlKwamrhL0bi9HzaDnFNDBvqQ6Sz0ldUTtedpYytgS5AZ3_kyes2rChjaZuljS3N1HduyYxTVkm7Cha20N9oKcnl3m8e_U_euM1e5RT1k5MSN5n26tFC2-Qdq3s2-hhvwHkPyuk priority: 102 providerName: Directory of Open Access Journals – databaseName: Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwhV1Lb9NAEB5BucAB8cZQ0CJx4OI2fuzaPqEWURWkIA6tlNtqnxSpsUscC_XGT-A38kuY2axNI16HSFG8iTb-Zma_2R1_A_DS5BpRL0VamQYTFK-LtNHcpU7RU5wWX-FZmPkHcXxavl_wRdxw62NZ5RgTQ6C2naE98n2kvpyKIurq9cWXlLpG0elqbKFxHW6QdBmVdFWLKeEStOcZ5XyoegdXyozkGfkeCe81WytREOz_E8v8vVjyKokNq9DRHbgd6SM72OB9F6659h7cuiIqeB--uuXHg3c_vn1HWkwYWtZvFGgvmWvPCOSeYazA_BWHM9VapiZ1TtZ5Nr806ONBw3lYsvWg3coM5x3-Fhsrt1qGhjtGTbakCp9V_wBOj96evDlOY3uF1PCibFKlSCqysFzpwnphvbdVphxHimTpvE07zx1XpbZlZpBVWl743Al0YG8xq3bFQ9hpu9Y9BuYFSdxmRjjvS8Vn2hjf1HWjnOYZpkwJ7I23WpqoPU4tMM7lmIMQNpKwkQGbBF5NX7jYyG78feghYTcNI73s8EG3-iSj-0mD82ocb4wqKGppPbO5U0U9s6LBxTtLYHdEXkYn7uUvk0vgxXQZ3Y_OVFTruoHGIAOd1RXN49HGUKaZIEGgJkB5AtWWCW1NdftK-_ksSHzXpKgpygT2g7H97x7I-eFJHt49-fcfeQo3cyrKCfXGu7CzXg3uGbKqtX4eXOcnUoAiFA priority: 102 providerName: ProQuest – databaseName: Wiley Online Library Open Access dbid: 24P link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3JbtRAEG1BuMABhd1JQI3EgYvDeGnbfUwiooA0KIdE4tbqpZogZWw0Hkvklk_gE_gWPoUvSVV70QyLEIeRrHHZavn1q66yq14z9sqmBlHPi7i0EhMUb7JYGgExaOridPgLvTDzD8XJef7-oxirCakXpteHmF64ETOCvyaCa9OukRzXvYTEFsU-yejJ2-wONdiSfH6an47OuKDXnqEncjAe1H2omOeXG2wsTEG__09B5--1k-sxbViUjrfZ_SGa5Ac9_A_YLagfsntrGoOP2FdYnB68-3n9DaNkgtTxthekveJQXxDmLUfXgeksmnNdO64nsU7eeD6_skj5IOncLX58X3UGlra7bPBmfKzkqjlO5NGL8gVV_Czbx-z8-O3Z0Uk8bLcQW5HlMtaapCMzJ7TJnC-c965MNAgMmRx9fzPgBQidG5cnFqNMJzKfQoGE9g6zbMiesK26qeEZ474gydvEFuB9rsXMWOtlVUkNRiSYQkVsf3zWyg5a5LQlxqUacxICRxE4KoATsdfTBV96GY6_mx4SeJMZ6WeHP5rlJzXQUVkclwQhrc7IixkzcynorJq5QuJinkRsb4ReDaRuFWZfgupyqjJiL6fTSEf6xqJraDqywYh0VpU0jqf9TJlGggEDbQqURqzcmEMbQ908U3--CJLfFSlsFnnE3oTZ9q9noOaHZ2k42vnvK3bZ3ZTqdkJJ8h7bWi07eI6B18q8CNS6AbxyJsg priority: 102 providerName: Wiley-Blackwell |
| Title | emPAI‐assisted strategy enhances screening and assessment of Mycobacterium tuberculosis infection serological markers |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2F1751-7915.13829 https://www.ncbi.nlm.nih.gov/pubmed/34173722 https://www.proquest.com/docview/2555199787 https://www.proquest.com/docview/2545608729 https://pubmed.ncbi.nlm.nih.gov/PMC8313264 https://doaj.org/article/cff49e59ca30400bb0d2ea380d692121 |
| Volume | 14 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Lb9NAEB7R9gIHxBuXEi0SBy4O8WP9OCDUoIaClCpCjZSbtc8WKbFpHlJz4yfwE_gt_BR-CTMb2zQiCA6xonhirXZmdr7xzn4D8FKFErUeJ36qckxQrIz8XHLjG0GnODV-3FmY4VlyOo4_TvjkdzugegIXO1M76ic1nk-711frt-jwb5qqHIyAAdEu8i4R6uV7cIBhKSUvHcbtlkJCL0Dd6chauOb52fGArRDlmPx3wc8_qyhvolsXngb34G6NK9nxxhDuwy1TPoA7N9gGH8K1mY2OP_z8-g3xMilXs8WGmnbNTHlJ2l8wXEQwsUVxJkrNREvbySrLhmuFzu_InVezH9-XK2nmajWt8GGsqekqGZp0s56yGdX-zBePYDw4OX936teNF3zFozj3hSASyUhzISNtE22tTgNhOIInTTtx0lhuuIiljgOFeFPzyIYmQde2GvNtEz2G_bIqzVNgNiHy20AlxtpY8J5UyuZZlgsjeYDJlAfdZq4LVbOSU3OMadFkJ6ScgpRTOOV48Kr9w5cNIcffRfukvFaMmLTdD9X8oqgds1A4rtzwXImI1jMpezo0Isp6OskxrAceHDWqLxrrLDAP41Shk6UevGhvo2PSbosoTbUiGcSmvSylcTzZWEo7EoQO1B4o9CDdsqGtoW7fKT9fOvLvjLg2k9iD187a_jUHxbB_Hrpvh_8_cc_gdkilO64q-Qj2l_OVeY7Yayk7sBfGI7ymkxSv2eB9Bw76J2ejTx33NqPjfO4X2usvaQ |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3JbtRAEC2FcAAOiDU4BGgkkLg48dZeDgglQDRDMhGHiTQ30ytBytjJLIrmxifwJXwUX0JVj20yYjvlYMmy21a7a-lX7upXAC9UJFHqSepnqsAAxcrYLyQ3vhG0i1Pj4fbCDI7S3nHyYcRHa_C93QtDaZWtT3SOWteK_pHvIPTllBSRZ2_Ozn2qGkWrq20JjaVaHJjFBYZs09f9dyjfl1G0_374tuc3VQV8xeOk8IUghsRYcyFjbVNtrc5CYTgiA03LTNJYbrhIpE5ChWBK89hGJkW9tRqDSRPje6_BdZx4A7KobNQFeCn9Y23ogyhbCGfmkOgg-TYR_RUrM58rEPAnVPt7cuZl0Oxmvf07cLuBq2x3qV93Yc1U9-DWJRLD-3Bhxh93-z--fkMYTjqj2XTJeLtgpjohpZoy9E0YL2NzJirNRMcGymrLBguFPsVxRs_HbDaXZqLmpzW-i7WZYhVDQ2m9NBtTRtFk-gCOr2TgH8J6VVfmETCbEqVuqFJjbSJ4IJWyRZ4XwkgeYojmwXY71KVquM6p5MZp2cY8JJuSZFM62XjwqnvgbEnz8femeyS7rhnxc7sL9eRz2Zh7qbBfheGFEjF5SSkDHRkR54FOCwQLoQdbreTLxmlMy18q7sHz7jaaO63hiMrUc2qDiDfIM-rHxlJRup4gIKGiQ5EH2YoKrXR19U715cRRiufE4JkmHuw4ZfvfGJSDvWHkzjb__SHP4EZvODgsD_tHB4_hZkQJQS7XeQvWZ5O5eYKIbiafOjNi8Omq7fYncsxfiw |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEB6VIiE4IN4YCiwSSFzcxI_144BQS4kaSqoeWik3s09aqbHbOFGVGz-B38PP4Zcws7FNI16nHixZ9tpa7zz2G-_sNwCvVChR6nHipyrHAMXKyM8lN74RtItT4-H2woz2k92j-OOYj9fge7sXhtIqW5_oHLWuFP0j7yH05ZQUkaU926RFHOwM3p2d-1RBilZa23IaSxXZM4sLDN_qt8MdlPXrMBx8OHy_6zcVBnzFozj3hSC2xEhzISNtE22tTgNhOKIETUtO0lhuuIiljgOFwErzyIYmQR22GgNLE-F7r8H1NOIB2Vg67oK9hP63NlRClDmEs3RA1JB8k0j_8pVZ0BUL-BPC_T1R8zKAdjPg4A7cbqAr21rq2l1YM-U9uHWJ0PA-XJjJwdbwx9dvCMlJfzSrl-y3C2bKY1KwmqGfwtgZmzNRaiY6ZlBWWTZaKPQvjj96PmGzuTRTNT-t8F2szRorGRpN67HZhLKLpvUDOLqSgX8I62VVmsfAbEL0uoFKjLWx4H2plM2zLBdG8gDDNQ8226EuVMN7TuU3Tos2_iHZFCSbwsnGgzfdA2dLyo-_N90m2XXNiKvbXaimX4rG9AuF_coNz5WIyGNK2dehEVHW10mOwCHwYKOVfNE4kLr4pe4evOxuo-nTeo4oTTWnNoh--1lK_Xi0VJSuJwhOqABR6EG6okIrXV29U54cO3rxjNg8k9iDnlO2_41BMdo-DN3Zk39_yAu4gRZbfBru7z2FmyHlBrm05w1Yn03n5hmCu5l87qyIweerNtufvz9jwQ |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=emPAI%E2%80%90assisted+strategy+enhances+screening+and+assessment+of+Mycobacterium%C2%A0tuberculosis+infection+serological+markers&rft.jtitle=Microbial+biotechnology&rft.au=Ma%2C+Guorong&rft.au=Wang%2C+Pei&rft.au=Yang%2C+Yanhui&rft.au=Wang%2C+Wei&rft.date=2021-07-01&rft.issn=1751-7915&rft.eissn=1751-7915&rft.volume=14&rft.issue=4&rft.spage=1827&rft.epage=1838&rft_id=info:doi/10.1111%2F1751-7915.13829&rft.externalDBID=n%2Fa&rft.externalDocID=10_1111_1751_7915_13829 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1751-7915&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1751-7915&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1751-7915&client=summon |