Reduced MAGI3 level by HPV18E6 contributes to Wnt/β‐catenin signaling activation and cervical cancer progression

• Published in: FEBS open bio Vol. 11; no. 11; pp. 3051 - 3062
• Main Authors: Yang, Zhuoli, Liu, Hua, Song, Ran, Lu, Wenxiu, Wang, Haibo, Gu, Siyu, Cao, Xuedi, Chen, Yibin, Liang, Jihuan, Qin, Qiong, Yang, Xiaomei, Feng, Duiping, He, Junqi
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2021; John Wiley and Sons Inc; Wiley
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; beta Catenin - metabolism; Biotechnology industry; Catenin; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cervical cancer; Cervix; China; Female; Gene Expression Regulation, Neoplastic; Genomes; Guanylate kinase; HPV; Human papillomavirus; Human papillomavirus 18 - metabolism; Human papillomavirus 18 - pathogenicity; Humans; Infections; invasion; Invasiveness; Kinases; MAGI3; Membrane proteins; Membrane Proteins - genetics; Membrane Proteins - metabolism; Neoplasm Invasiveness - genetics; Oncogene Proteins, Viral - metabolism; Papillomavirus Infections; PDZ; Plasmids; Postsynaptic density proteins; Proteins; Repressor Proteins - metabolism; Signal transduction; Transcription Factors - metabolism; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - physiopathology; Vaccines; Wnt protein; Wnt Signaling Pathway - genetics; Wnt Signaling Pathway - physiology; Wnt/β‐catenin; China; Beijing China; United States > US; invasion; cervical cancer; MAGI3; Wnt/β-catenin; PDZ; HPV
• DOI: 10.1002/2211-5463.13298
• ISSN: 2211-5463

Human papillomavirus type 18 (HPV18) has high carcinogenic power in invasive cervical cancer (ICC) development. However, the underlying mechanism remains elusive. The carcinogenic properties of HPV18 require the PDZ‐binding motif of its E6 oncoprotein (HPV18 E6) to degrade its target PSD95/Dlg/ZO‐1 (PDZ) proteins. In this study, we demonstrated that the PDZ protein membrane‐associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3) inhibited the Wnt/β‐catenin pathway, and subsequently cervical cancer (CC) cell migration and invasion, via decreasing β‐catenin levels. By reducing MAGI3 protein levels, HPV18 E6 promoted CC cell migration and invasion through activation of Wnt/β‐catenin signaling. Furthermore, HPV18 rather than HPV16 was preferentially associated with the downregulation of MAGI3 and activation of the Wnt/β‐catenin pathway in CC. These findings shed light on the mechanism that gives HPV18 its high carcinogenic potential in CC progression. By reducing the level of MAGI3 protein, HPV18 E6 promotes cervical cancer cell migration and invasion through activation of Wnt/β‐catenin signaling. Furthermore, HPV18 rather than HPV16 is preferentially associated with downregulation of MAGI3 and activation of the Wnt/β‐catenin pathway in cervical cancer. These findings shed light on the mechanism that gives HPV18 its high carcinogenic potential in cervical cancer progression.