A combined biochemical screen and TILLING approach identifies mutations in Sorghum bicolor L. Moench resulting in acyanogenic forage production - Blomstedt, Cecilia K.; Gleadow, Roslyn M.; O'Donnell, Natalie; Naur, Peter; Jensen, Kenneth; Laursen, Tomas; Olsen, Carl Erik; Stuart, Peter; Hamill, John D.; Møller, Birger Lindberg; Neale, Alan D. (Plant biotechnology journal, 2012)
Keywords: Animal Feed; Animals; Biological and medical sciences; biosynthesis; Biosynthetic Pathways; Biotechnology; Biotechnology - methods; Blotting, Western; Crosses, Genetic; cyanide toxicity; CYP79A1; cytochrome P-450; Cytochrome P-450 Enzyme System; Cytochrome P-450 Enzyme System - genetics; enzymes; enzymology; Ethyl Methanesulfonate; forage production; Fundamental and applied biological sciences. Psychology; gene regulation; genes; genetic techniques and protocols; genetics; Genome, Plant; Genome, Plant - genetics; glucosides; Glycosides; Glycosides - metabolism; Humans; hydrogen cyanide; Hydrogen Cyanide - metabolism; leaves; mature plants; metabolism; methods; Microsomes; Microsomes - enzymology; Models, Molecular; molecular models; Mutagenesis; Mutagenesis - genetics; mutants; mutation; Mutation - genetics; mutations; NADP; NADP - metabolism; Nitriles; Nitriles - metabolism; nitrogen metabolism; Phenotype; screening; seedlings; seeds; Sorghum; Sorghum - enzymology; Sorghum - genetics; Sorghum bicolor; Structural Homology, Protein; Plant production; Monocotyledones; Toxicity; nitrogen metabolism; Biochemistry; Cyanides; Nitrogen; Gene expression; Metabolism; CYP79A1; Regulation(control); Sorghum bicolor; mutations; Gene; Gramineae; gene regulation; cyanide toxicity; Angiospermae; Spermatophyta; Mutation; Fodder crop