Enhancing the capability of Klebsiella pneumoniae to produce 1, 3‐propanediol by overexpression and regulation through CRISPR‐dCas9

• Published in: Microbial biotechnology Vol. 15; no. 7; pp. 2112 - 2125
• Main Authors: Wang, Xin, Zhang, Lin, Liang, Shaoxiong, Yin, Ying, Wang, Pan, Li, Yicao, Chin, Wee Shong, Xu, Jianwei, Wen, Jianping
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.07.2022; John Wiley and Sons Inc; Wiley
• Subjects: By products; Carbon; CRISPR; Dehydrogenases; Enzymes; Fermentation; Gene expression; Gene manipulation; Genes; Genetic engineering; Glycerol; Klebsiella; Klebsiella pneumoniae; Metabolism; Microorganisms; Oxidation; Plasmids; Regulation; Standard deviation

Summary Klebsiella pneumoniae is a common strain of bacterial fermentation to produce 1, 3‐propanediol (1, 3‐PDO). In general, the production of 1, 3‐PDO by wild‐type K. pneumoniae is relatively low. Therefore, a new gene manipulation of K. pneumoniae was developed to improve the production of 1, 3‐...