Increasing the Content of High-Content Screening An Overview

• Published in: Journal of biomolecular screening Vol. 19; no. 5; pp. 640 - 650
• Main Authors: Singh, Shantanu, Carpenter, Anne E., Genovesio, Auguste
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.06.2014
• Subjects: Biological Assay; Chemistry, Pharmaceutical - methods; Drug Discovery; High-Throughput Screening Assays - methods; Multivariate Analysis; Normal Distribution; Phenotype; Support Vector Machine; statistical analyses; phenotypic drug discovery; Cell-based assays; image analysis; high-content screening

Target-based high-throughput screening (HTS) has recently been critiqued for its relatively poor yield compared to phenotypic screening approaches. One type of phenotypic screening, image-based high-content screening (HCS), has been seen as particularly promising. In this article, we assess whether...