Single-cell transcriptomic characterization of microscopic colitis

• Published in: Nature communications Vol. 16; no. 1; pp. 4618 - 16
• Main Authors: Halvorsen, Stefan, Thomas, Molly, Mino-Kenudson, Mari, Kinowaki, Yuko, Burke, Kristin E., Morgan, David, Miller, Kaia C., Williams, Katherine M., Gurung, Jenny, McGoldrick, Jessica, Hopton, Megan, Hoppe, Brooke, Samanta, Nandini, Martin, Sidney, Tirard, Alice, Arnold, Benjamin Y., Tantivit, Jessica, Yarze, Joseph, Staller, Kyle, Chung, Daniel C., Villani, Alexandra-Chloé, Sassi, Slim, Khalili, Hamed
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.05.2025; Nature Publishing Group; Nature Portfolio
• Subjects: 13/51; 14/32; 14/63; 38/91; 631/250/1619; 631/250/347; 631/337/2019; 692/420/256/2515; 692/699/1503; Animals; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; CD8 antigen; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - metabolism; Colitis; Colitis, Microscopic - genetics; Colitis, Microscopic - immunology; Colitis, Microscopic - metabolism; Colitis, Microscopic - pathology; Colon - immunology; Colon - metabolism; Colon - pathology; Cytotoxicity; Diarrhea; Female; Gene Expression Profiling; Gene sequencing; Humanities and Social Sciences; Humans; Immune system; Immunological memory; Immunoregulation; Inflammatory bowel disease; Inflammatory diseases; Interleukin-10 - genetics; Interleukin-10 - immunology; Interleukin-10 - metabolism; Intestinal Mucosa - immunology; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Intestine; Large intestine; Localization; Lymphocytes; Lymphocytes T; Male; Memory cells; Mice; Mice, Inbred C57BL; multidisciplinary; Older people; Pathogenesis; Phenotypes; Science; Science (multidisciplinary); Sequence analysis; Single-Cell Analysis - methods; T cell receptors; T-Lymphocytes, Regulatory - immunology; Transcriptome; Transcriptomics; Up-regulation

Microscopic colitis (MC) is a chronic inflammatory disease of the large intestine and a common cause of chronic diarrhea in older adults. Here, we use single-cell RNA sequencing analysis of colonic mucosal tissue to build a cellular and molecular model for MC. Our results show that in MC, there is a...